Construct: ORF ccsbBroad304_01668

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001433.1_s304c1, BRDN0000412188
Derived from:
ccsbBroadEn_01668
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
THPO (7066)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01668
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7066 THPO thrombopoietin NM_001177597.2 100% 100%
2 human 7066 THPO thrombopoietin NM_001290022.1 100% 100%
3 human 7066 THPO thrombopoietin NM_000460.4 98.8% 98.8% 394_405del
4 human 7066 THPO thrombopoietin NM_001289998.1 98.8% 98.8% 394_405del
5 human 7066 THPO thrombopoietin NM_001290028.1 98.8% 98.8% 394_405del
6 human 7066 THPO thrombopoietin NM_001177598.2 91.4% 43% 393_394insCAGGG;957_958ins85
7 human 7066 THPO thrombopoietin NM_001290026.1 91.4% 43% 393_394insCAGGG;957_958ins85
8 human 7066 THPO thrombopoietin NM_001289997.1 79.8% 50.9% 394_405del;475_476ins116;858_859ins85
9 human 7066 THPO thrombopoietin NM_001290027.1 79.8% 50.9% 394_405del;475_476ins116;858_859ins85
10 human 7066 THPO thrombopoietin NM_001290003.1 70.7% 70.7% 1_420del;814_825del
11 human 7066 THPO thrombopoietin XM_017007107.1 57.6% 37.4% 1_420del;883_884ins116;1266_1267ins85
12 mouse 21832 Thpo thrombopoietin XM_017316933.1 23.1% 23% (many diffs)
13 mouse 21832 Thpo thrombopoietin XM_017316932.1 22.4% 21.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1116
ORF length:
1047
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggagctgact gaattgctcc tcgtggtcat gcttctccta actgcaaggc
121taacgctgtc cagcccggct cctcctgctt gtgacctccg agtcctcagt aaactgcttc
181gtgactccca tgtccttcac agcagactga gccagtgccc agaggttcac cctttgccta
241cacctgtcct gctgcctgct gtggacttta gcttgggaga atggaaaacc cagatggagg
301agaccaaggc acaggacatt ctgggagcag tgacccttct gctggaggga gtgatggcag
361cacggggaca actgggaccc acttgcctct catccctcct ggggcagctt tctggacagg
421tccgtctcct ccttggggcc ctgcagagcc tccttggaac ccagggcagg accacagctc
481acaaggatcc caatgccatc ttcctgagct tccaacacct gctccgagga aaggtgcgtt
541tcctgatgct tgtaggaggg tccaccctct gcgtcaggcg ggccccaccc accacagctg
601tccccagcag aacctctcta gtcctcacac tgaacgagct cccaaacagg acttctggat
661tgttggagac aaacttcact gcctcagcca gaactactgg ctctgggctt ctgaagtggc
721agcagggatt cagagccaag attcctggtc tgctgaacca aacctccagg tccctggacc
781aaatccccgg atacctgaac aggatacacg aactcttgaa tggaactcgt ggactctttc
841ctggaccctc acgcaggacc ctaggagccc cggacatttc ctcaggaaca tcagacacag
901gctccctgcc acccaacctc cagcctggat attctccttc cccaacccat cctcctactg
961gacagtatac gctcttccct cttccaccca ccttgcccac ccctgtggtc cagctccacc
1021ccctgcttcc tgacccttct gctccaacgc ccacccctac cagccctctt ctaaacacat
1081cctacaccca ctcccagaat ctgtctcagg aagggttgcc aactttcttg tacaaagtgg
1141ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1201ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)