Construct: ORF ccsbBroad304_01686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004706.1_s304c1, BRDN0000408722
- Derived from:
- ccsbBroadEn_01686
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TNFAIP3 (7128)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01686
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | NM_001270507.2 | 99.9% | 76.1% | 1757delC |
| 2 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | NM_001270508.2 | 99.9% | 76.1% | 1757delC |
| 3 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | NM_006290.4 | 99.9% | 76.1% | 1757delC |
| 4 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | XM_005267119.1 | 99.9% | 76.1% | 1757delC |
| 5 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | XM_011536095.1 | 99.9% | 76.1% | 1757delC |
| 6 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | XM_024446532.1 | 99.9% | 76.1% | 1757delC |
| 7 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | XM_024446533.1 | 99.9% | 76.1% | 1757delC |
| 8 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | XM_011536096.2 | 87.1% | 83.9% | 1757delC;1906_1907ins182;2067_2068ins121 |
| 9 | human | 7128 | TNFAIP3 | TNF alpha induced protein 3 | XM_006715555.1 | 72.9% | 49.1% | 0_1ins639;1118delC |
| 10 | mouse | 21929 | Tnfaip3 | tumor necrosis factor, alph... | NM_009397.3 | 83.6% | 68.5% | (many diffs) |
| 11 | mouse | 21929 | Tnfaip3 | tumor necrosis factor, alph... | XM_006512702.2 | 83.6% | 68.5% | (many diffs) |
| 12 | mouse | 21929 | Tnfaip3 | tumor necrosis factor, alph... | NM_001166402.1 | 79.9% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2154
- ORF length:
- 2085
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgaacaa gtccttcctc aggctttgta tttgagcaat atgcggaaag 121 ctgtgaagat acgggagaga actccagaag acatttttaa acctactaat gggatcattc 181 atcattttaa aaccatgcac cgatacacac tggaaatgtt cagaacttgc cagttttgtc 241 ctcagtttcg ggagatcatc cacaaagccc tcatcgacag aaacatccag gccaccctgg 301 aaagccagaa gaaactcaac tggtgtcgag aagtccggaa gcttgtggcg ctgaaaacga 361 acggtgacgg caattgcctc atgcatgcca cttctcagta catgtggggc gttcaggaca 421 cagacttggt actgaggaag gcgctgttca gcacgctcaa ggaaacagac acacgcaact 481 ttaaattccg ctggcaactg gagtctctca aatctcagga atttgttgaa acggggcttt 541 gctatgatac tcggaactgg aatgatgaat gggacaatct tatcaaaatg gcttccacag 601 acacacccat ggcccgaagt ggacttcagt acaactcact ggaagaaata cacatatttg 661 tcctttgcaa catcctcaga aggccaatca ttgtcatttc agacaaaatg ctaagaagtt 721 tggaatcagg ttccaatttc gcccctttga aagtgggtgg aatttacttg cctctccact 781 ggcctgccca ggaatgctac agatacccca ttgttctcgg ctatgacagc catcattttg 841 tacccttggt gaccctgaag gacagtgggc ctgaaatccg agctgttcca cttgttaaca 901 gagaccgggg aagatttgaa gacttaaaag ttcacttttt gacagatcct gaaaatgaga 961 tgaaggagaa gctcttaaaa gagtacttaa tggtgataga aatccccgtc caaggctggg 1021 accatggcac aactcatctc atcaatgccg caaagttgga tgaagctaac ttaccaaaag 1081 aaatcaatct ggtagatgat tactttgaac ttgttcagca tgagtacaag aaatggcagg 1141 aaaacagcga gcaggggagg agagaggggc acgcccagaa tcccatggaa ccttccgtgc 1201 cccagctttc tctcatggat gtaaaatgtg aaacgcccaa ctgccccttc ttcatgtctg 1261 tgaacaccca gcctttatgc catgagtgct cagagaggcg gcaaaagaat caaaacaaac 1321 tcccaaagct gaactccaag ccgggccctg aggggctccc tggcatggcg ctcggggcct 1381 ctcggggaga agcctatgag cccttggcgt ggaaccctga ggagtccact ggggggcctc 1441 attcggcccc accgacagca cccagccctt ttctgttcag tgagaccact gccatgaagt 1501 gcaggagccc cggctgcccc ttcacactga atgtgcagca caacggattt tgtgaacgtt 1561 gccacaacgc ccggcaactt cacgccagcc acgccccaga ccacacaagg cacttggatc 1621 ccgggaagtg ccaagcctgc ctccaggatg ttaccaggac atttaatggg atctgcagta 1681 cttgcttcaa aaggactaca gcagaggcct cctccagcct cagcaccagc ctccctcctt 1741 cctgtcacca gcgttccaag tcagatccct cgcggctcgt ccgGAGCCCC TCCCCGCATT 1801 CTTGCCACAG AGCTGGAAAC GACGCCCTGC TGGCTGCCTG TCTCAAGCTG CACGGACTCC 1861 TGGGGACAGG ACGGGGACGA GCAAGTGCAG AAAAGCCGGC TGCGTGTATT TTGGGACTCC 1921 AGAAAACAAG GGCTTTTGCA CACTGTGTTT CATCGAGTAC AGAGAAAACA AACATTTTGC 1981 TGCTGCCTCA GGGAAAGTCA GTCCCACAGC GTCCAGGTTC CAGAACACCA TTCCGTGCCT 2041 GGGGAGGGAA TGCGGCACCC TTGGAAGCAC CATGTTTGAA GGATACTGCC AGAAGTGTTT 2101 CATTGAAGCT CAGAATCAGA GATTTCATGA GGCCAAAAGG ACAGAAGAGC AACTGAGATC 2161 GAGCCAGCGC AGAGATGTGC CTCGAACCAC ACAAAGCACC TCAAGGCCCA AGTGCGCCCG 2221 GGCCTCCTGC AAGAACATCC TGGCCTGCCG CAGCGAGGAG CTCTGCATGG AGTGTCAGCA 2281 TCCCAACCAG AGGATGGGCC CTGGGGCCCA CCGGGGTGAG CCTGCCCCCG AAGACCCCCC 2341 CAAGCAGCGT TGCCGGGCCC CCGCCTGTGA TCATTTTGGC AATGCCAAGT GCAACGGCTA 2401 CTGCAACGAA TGCTTTCAGT TCAAGCAGAT GTATGGCTTG CCAACTTTCT TGTACaaagt 2461 ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag 2521 cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta