Construct: ORF ccsbBroad304_01713

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006983.1_s304c1, BRDN0000408742
Derived from:
ccsbBroadEn_01713
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRPC1 (7220)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01713
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7220 TRPC1 transient receptor potentia... NM_003304.4 100% 100%
2 human 7220 TRPC1 transient receptor potentia... NM_001251845.2 95.7% 95.7% 328_429del
3 human 7220 TRPC1 transient receptor potentia... XM_005247738.4 89.5% 85.4% (many diffs)
4 human 7220 TRPC1 transient receptor potentia... XM_017007121.2 88% 88% 0_1ins183;145_246del
5 human 7220 TRPC1 transient receptor potentia... XM_005247739.2 87% 85.3% 0_1ins139;32_33ins155
6 human 7220 TRPC1 transient receptor potentia... XR_001740246.1 62.5% (many diffs)
7 human 7220 TRPC1 transient receptor potentia... XR_241506.4 48.7% (many diffs)
8 mouse 22063 Trpc1 transient receptor potentia... NM_001311123.1 88.9% 97.6% (many diffs)
9 mouse 22063 Trpc1 transient receptor potentia... NM_011643.3 85.2% 93.5% (many diffs)
10 mouse 22063 Trpc1 transient receptor potentia... XM_006511053.3 84.4% 92.7% (many diffs)
11 mouse 22063 Trpc1 transient receptor potentia... XM_006511055.3 80.9% 85.4% (many diffs)
12 mouse 22063 Trpc1 transient receptor potentia... XM_006511056.3 78.5% 85.3% (many diffs)
13 mouse 22063 Trpc1 transient receptor potentia... XM_006511057.2 76.8% 85.3% (many diffs)
14 mouse 22063 Trpc1 transient receptor potentia... XM_011242717.2 46% 49% (many diffs)
15 mouse 22063 Trpc1 transient receptor potentia... XR_870756.2 38.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2346
ORF length:
2277
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gatggcggcc ctgtacccga gcacggacct ctcgggcgcc tcctcctcct
121ccctgccttc ctctccatcc tcttcctcgc cgaacgaggt gatggcgctg aaggatgtgc
181gggaggtgaa ggaggagaat acgctgaatg agaagctttt cttgctggcg tgcgacaagg
241gtgactatta tatggttaaa aagattttgg aggaaaacag ttcaggtgac ttgaacataa
301attgcgtaga tgtgcttggg agaaatgctg ttaccataac tattgaaaac gaaaacttgg
361atatactgca gcttcttttg gactacggtt gtcagaaact aatggaacga attcagaatc
421ctgagtattc aacaactatg gatgttgcac ctgtcatttt agctgctcat cgtaacaact
481atgaaattct tacaatgctc ttaaaacagg atgtatctct acccaagccc catgcagttg
541gctgtgaatg cacattgtgt tctgcaaaaa acaaaaagga tagcctccgg cattccaggt
601ttcgtcttga tatatatcga tgtttggcca gtccagctct aataatgtta acagaggagg
661atccaattct gagagcattt gaacttagtg ctgatttaaa agaactaagt cttgtggagg
721tggaattcag gaatgattat gaggaactag cccggcaatg taaaatgttt gctaaggatt
781tacttgcaca agcccggaat tctcgtgaat tggaagttat tctaaaccat acgtctagtg
841acgagcctct tgacaaacgg ggattattag aagaaagaat gaatttaagt cgtctaaaac
901ttgctatcaa atataaccag aaagagtttg tctcccagtc taactgccag cagttcctga
961acactgtttg gtttggacag atgtcgggtt accgacgcaa gcccacctgt aagaagataa
1021tgactgtttt gacagtaggc atcttttggc cagttttgtc actttgttat ttgatagctc
1081ccaaatctca gtttggcaga atcattcaca caccttttat gaaatttatc attcatggag
1141catcatattt cacatttctg ctgttgctta atctatactc tcttgtctac aatgaggata
1201agaaaaacac aatggggcca gcccttgaaa gaatagacta tcttcttatt ctgtggatta
1261ttgggatgat ttggtcagac attaaaagac tctggtatga agggttggaa gactttttag
1321aagaatctcg taatcaactc agttttgtca tgaattctct ttatttggca acctttgccc
1381tcaaagtggt tgctcacaac aagtttcatg attttgctga tcggaaggat tgggatgcat
1441tccatcctac actggtggca gaagggcttt ttgcatttgc aaatgttcta agttatcttc
1501gtctcttttt tatgtataca accagctcta tcttgggtcc attacagatt tcaatgggac
1561agatgttaca agattttgga aaatttcttg ggatgtttct tcttgttttg ttttctttca
1621caattggact gacacaactg tatgataaag gatatacttc aaaggagcag aaggactgtg
1681taggcatctt ctgtgaacag caaagcaatg ataccttcca ttcgttcatt ggcacctgct
1741ttgctttgtt ctggtatatt ttctccttag cgcatgtggc aatctttgtc acaagattta
1801gctatggaga agaactgcag tcctttgtgg gagctgtcat tgttggtaca tacaatgtcg
1861tggttgtgat tgtgcttacc aaactgctgg tggcaatgct tcataaaagc tttcagttga
1921tagcaaatca tgaagacaaa gaatggaagt ttgctcgagc aaaattatgg cttagctact
1981ttgatgacaa atgtacgtta cctccacctt tcaacatcat tccctcacca aagactatct
2041gctatatgat tagtagcctc agtaagtgga tttgctctca tacatcaaaa ggcaaggtca
2101aacggcaaaa cagtttaaag gaatggagga atttgaaaca gaagagagat gaaaactatc
2161aaaaagtgat gtgctgccta gtgcatcgtt acttgacttc catgagacag aagatgcaaa
2221gtacagatca ggcaactgtg gaaaatctaa acgaactgcg ccaagatctg tcaaaattcc
2281gaaatgaaat aagggattta cttggctttc ggacttctaa atatgctatg ttttatccaa
2341gaaatttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2401gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2461acctctggat ta

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