Construct: ORF ccsbBroad304_01714

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002597.1_s304c1, BRDN0000462179
Derived from:
ccsbBroadEn_01714
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRPC3 (7222)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01714
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7222 TRPC3 transient receptor potentia... NM_003305.2 100% 100%
2 human 7222 TRPC3 transient receptor potentia... NM_001130698.2 92% 92% 1_219del
3 human 7222 TRPC3 transient receptor potentia... XM_011532218.3 91.9% 91.9% 1_219del;2544_2545insCAG
4 human 7222 TRPC3 transient receptor potentia... XM_011532217.3 90.5% 90.5% 1_219del;2463_2510del
5 human 7222 TRPC3 transient receptor potentia... XM_017008578.2 90.3% 90.3% 1_219del;2463_2510del;2592_2593insCAG
6 human 7222 TRPC3 transient receptor potentia... NM_001366479.2 89% 89% 1_219del;2462_2463ins84
7 mouse 22065 Trpc3 transient receptor potentia... NM_019510.2 81.7% 89.3% (many diffs)
8 mouse 22065 Trpc3 transient receptor potentia... XM_006535439.3 78.7% 86.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2613
ORF length:
2544
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggagggaagc ccatccctga gacgcatgac agtgatgcgg gagaagggcc
121ggcgccaggc tgtcaggggc ccggccttca tgttcaatga ccgcggcacc agcctcaccg
181ccgaggagga gcgcttcctc gacgccgccg agtacggcaa catcccagtg gtgcgcaaga
241tgctggagga gtccaagacg ctgaacgtca actgcgtgga ctacatgggc cagaacgcgc
301tgcagctggc tgtgggcaac gagcacctgg aggtgaccga gctgctgctc aagaaggaga
361acctggcgcg cattggcgac gccctgctgc tcgccatcag caagggctac gtgcgcatcg
421tagaggccat cctcaaccac cctggcttcg cggccagcaa gcgtctcact ctgagcccct
481gtgagcagga gctgcaggac gacgacttct acgcttacga cgaggacggc acgcgcttct
541cgccggacat cacccccatc atcctggcgg cgcactgcca gaaatacgaa gtggtgcaca
601tgctgctgat gaagggtgcc aggatcgagc ggccgcacga ctatttctgc aagtgcgggg
661actgcatgga gaagcagagg cacgactcct tcagccactc acgctcgagg atcaatgcct
721acaaggggct ggccagcccg gcttacctct cattgtccag cgaggacccg gtgcttacgg
781ccctagagct cagcaacgag ctggccaagc tggccaacat agagaaggag ttcaagaatg
841actatcggaa gctctccatg caatgcaaag actttgtagt gggtgtgctg gatctctgcc
901gagactcaga agaggtagaa gccattctga atggagatct ggaatcagca gagcctctgg
961aggtacacag gcacaaagct tcattaagtc gtgtcaaact tgccattaag tatgaagtca
1021aaaagtttgt ggctcatccc aactgccagc agcagctctt gacgatctgg tatgagaacc
1081tctcaggcct aagggagcag accatagcta tcaagtgtct cgttgtgctg gtcgtggccc
1141tgggccttcc attcctggcc attggctact ggatcgcacc ttgcagcagg ctggggaaaa
1201ttctgcgaag cccttttatg aagtttgtag cacatgcagc ttctttcatc atcttcctgg
1261gtctgcttgt gttcaatgcc tcagacaggt tcgaaggcat caccacgctg cccaatatca
1321cagttactga ctatcccaaa cagatcttca gggtgaaaac cacccagttt acatggactg
1381aaatgctaat tatggtctgg gttcttggaa tgatgtggtc tgaatgtaaa gagctctggc
1441tggaaggacc tagggaatac attttgcagt tgtggaatgt gcttgacttt gggatgctgt
1501ccatcttcat tgctgctttc acagccagat tcctagcttt ccttcaggca acgaaggcac
1561aacagtatgt ggacagttac gtccaagaga gtgacctcag tgaagtgaca ctcccaccag
1621agatacagta tttcacttat gctagagata aatggctccc ttctgaccct cagattatat
1681ctgaaggcct ttatgccata gctgttgtgc tcagcttctc tcggattgcg tacatcctcc
1741ctgcaaatga gagctttggc cccctgcaga tctctcttgg aaggactgta aaggacatat
1801tcaagttcat ggtcctcttt attatggtgt tttttgcctt tatgattggc atgttcatac
1861tttattctta ctaccttggg gctaaagtta atgctgcttt taccactgta gaagaaagtt
1921tcaagacttt attttggtca atatttgggt tgtctgaagt gacttccgtt gtgctcaaat
1981atgatcacaa attcatagaa aatattggat acgttcttta tggaatatac aatgtaacta
2041tggtggtcgt tttactcaac atgctaattg ctatgattaa tagctcatat caagaaattg
2101aggatgacag tgatgtagaa tggaagtttg ctcgttcaaa actttggtta tcctattttg
2161atgatggaaa aacattacct ccacctttca gtctagttcc tagtccaaaa tcatttgttt
2221atttcatcat gcgaattgtt aactttccca aatgcagaag gagaaggctt cagaaggata
2281tagaaatggg aatgggtaac tcaaagtcca ggttaaacct cttcactcag tctaactcaa
2341gagtttttga atcacacagt tttaacagca ttctcaatca gccaacacgt tatcagcaga
2401taatgaaaag acttataaag cggtatgttt tgaaagcaca agtagacaaa gaaaatgatg
2461aagttaatga aggtgaatta aaagaaatca agcaagatat ctccagcctt cgttatgaac
2521ttttggaaga caagagccaa gcaactgagg aattagccat tctaattcat aaacttagtg
2581agaaactgaa tcccagcatg ctgagatgtg aattgccaac tttcttgtac aaagtggttg
2641gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2701accggtggcg cgttaagtcg acaatcaacc tctggatta

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