Construct: ORF ccsbBroad304_01731
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013331.1_s304c1, BRDN0000409346
- Derived from:
- ccsbBroadEn_01731
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- U2AF1 (7307)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01731
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | NM_001320646.2 | 100% | 100% | |
| 2 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | NM_006758.2 | 100% | 100% | |
| 3 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | NM_001025203.1 | 96.4% | 97% | (many diffs) |
| 4 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | NM_001320648.2 | 96.4% | 97% | (many diffs) |
| 5 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | XM_011529743.3 | 86.2% | 86.2% | 247_248ins99 |
| 6 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | XM_017028219.1 | 86.2% | 86.2% | 247_248ins99 |
| 7 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | XM_017028220.1 | 82.7% | 83.3% | (many diffs) |
| 8 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | XM_017028468.2 | 82.7% | 83.3% | (many diffs) |
| 9 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | NM_001320650.2 | 81.7% | 75% | (many diffs) |
| 10 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | NM_001025204.1 | 69.5% | 69.5% | 0_1ins219 |
| 11 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | NM_001320651.1 | 69.5% | 69.5% | 0_1ins219 |
| 12 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | XM_024452037.1 | 69.5% | 69.5% | 0_1ins219 |
| 13 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | XM_024452038.1 | 69.5% | 69.5% | 0_1ins219 |
| 14 | human | 102724594 | U2AF1L5 | U2 small nuclear RNA auxili... | XM_024452039.1 | 69.5% | 69.5% | 0_1ins219 |
| 15 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | XM_024452129.1 | 69.5% | 69.5% | 0_1ins219 |
| 16 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | XM_024452130.1 | 69.5% | 69.5% | 0_1ins219 |
| 17 | human | 7307 | U2AF1 | U2 small nuclear RNA auxili... | XM_024452131.1 | 69.5% | 69.5% | 0_1ins219 |
| 18 | mouse | 108121 | U2af1 | U2 small nuclear ribonucleo... | NM_024187.4 | 90% | 99.5% | (many diffs) |
| 19 | mouse | 108121 | U2af1 | U2 small nuclear ribonucleo... | NM_001163769.1 | 86.5% | 96.6% | (many diffs) |
| 20 | mouse | 629480 | Gm6978 | predicted gene 6978 | XR_386288.3 | 73.7% | (many diffs) | |
| 21 | mouse | 108121 | U2af1 | U2 small nuclear ribonucleo... | XM_006523472.1 | 61.1% | 69.1% | (many diffs) |
| 22 | mouse | 108121 | U2af1 | U2 small nuclear ribonucleo... | XM_006523473.2 | 61.1% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 786
- ORF length:
- 720
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagtatctg gcctccatct tcggcaccga gaaagacaaa gtcaactgtt 121 cattttattt caaaattgga gcatgtcgtc atggagacag gtgctctcgg ttgcacaata 181 aaccgacgtt tagccagacc attgccctct tgaacattta ccgtaaccct caaaactctt 241 cccagtctgc tgacggtttg cgctgtgccg tgagcgatgt ggagatgcag gaacactatg 301 atgagttttt tgaggaggtt tttacagaaa tggaggagaa gtatggggaa gtagaggaga 361 tgaacgtctg tgacaacctg ggagaccacc tggtggggaa cgtgtacgtc aagtttcgcc 421 gtgaggaaga tgcggaaaag gctgtgattg acttgaataa ccgttggttt aatggacagc 481 cgatccacgc cgagctgtca cccgtgacgg acttcagaga agcctgctgc cgtcagtatg 541 agatgggaga atgcacacga ggcggcttct gcaacttcat gcatttgaag cccatttcca 601 gagagctgcg gcgggagctg tatggccgcc gtcgcaagaa gcatagatca agatcccgat 661 cccgggagcg tcgttctcgg tctagagacc gtggtcgtgg cggtggcggt ggcggtggtg 721 gaggtggcgg cggacgggag cgtgacagga ggcggtcgag agatcgtgaa agatctgggc 781 gattctgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 841 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 901 acctctggat ta