Construct: ORF ccsbBroad304_01745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010604.1_s304c1, BRDN0000402212
- Derived from:
- ccsbBroadEn_01745
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UCHL3 (7347)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01745
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | NM_006002.4 | 100% | 100% | |
| 2 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | XM_017020727.1 | 98.2% | 98.2% | 40_41insAGGTCACCAACC |
| 3 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | NM_001270952.1 | 84.3% | 84.3% | 0_1ins108 |
| 4 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | XM_011535212.1 | 78.2% | 78.2% | 475_666del |
| 5 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | XM_011535213.2 | 76.8% | 76.8% | 40_41insAGGTCACCAACC;463_654del |
| 6 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | XM_011535214.2 | 65.9% | 65.9% | 0_1ins108;367_558del |
| 7 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | XM_017020725.1 | 64.6% | 62.9% | (many diffs) |
| 8 | human | 7347 | UCHL3 | ubiquitin C-terminal hydrol... | XM_017020726.1 | 63.3% | 63.6% | (many diffs) |
| 9 | mouse | 50933 | Uchl3 | ubiquitin carboxyl-terminal... | NM_016723.2 | 90.4% | 98.2% | (many diffs) |
| 10 | mouse | 93841 | Uchl4 | ubiquitin carboxyl-terminal... | NM_033607.1 | 88.4% | 94.4% | (many diffs) |
| 11 | mouse | 50933 | Uchl3 | ubiquitin carboxyl-terminal... | XM_006519248.2 | 68.4% | 74.3% | (many diffs) |
| 12 | mouse | 50933 | Uchl3 | ubiquitin carboxyl-terminal... | XM_006519247.2 | 63.6% | 69.1% | (many diffs) |
| 13 | mouse | 50933 | Uchl3 | ubiquitin carboxyl-terminal... | XM_006519246.3 | 56.5% | 61.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gggtcaacgc tggctgccgc tggaggccaa tcccgaggtc accaaccagt 121 ttcttaaaca attaggtcta catcctaact ggcaattcgt tgatgtatat ggaatggatc 181 ctgaactcct tagcatggta ccaagaccag tctgtgcagt cttacttctc tttcctatta 241 cagaaaagta tgaagtattc agaacagaag aggaagaaaa aataaaatct cagggacaag 301 atgttacatc atcagtatat ttcatgaagc aaacaatcag caatgcctgt ggaacaattg 361 gactgattca tgctattgca aacaataaag acaagatgca ctttgaatct ggatcaacct 421 tgaaaaaatt cctggaggaa tctgtgtcaa tgagccctga agaacgagcc agatacctgg 481 agaactatga tgccatccga gttactcatg agaccagtgc ccatgaaggt cagactgagg 541 caccaagtat agatgagaaa gtagatcttc attttattgc attagttcat gtagatgggc 601 atctctatga attagatggg cggaagccat ttccaattaa ccatggtgaa actagtgatg 661 aaactttatt agaggatgcc atagaagttt gcaagaagtt tatggagcgc gaccctgatg 721 aactaagatt taatgcgatt gctctttctg cagcataccc aactttcttg tacaaagtgg 781 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 841 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta