Transcript: Human XM_011535214.2

PREDICTED: Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UCHL3 (7347)
Length:
1156
CDS:
248..1024

Additional Resources:

NCBI RefSeq record:
XM_011535214.2
NBCI Gene record:
UCHL3 (7347)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011535214.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007536 GCACCAAGTATAGATGAGAAA pLKO.1 806 CDS 100% 4.950 6.930 N UCHL3 n/a
2 TRCN0000320681 GCACCAAGTATAGATGAGAAA pLKO_005 806 CDS 100% 4.950 6.930 N UCHL3 n/a
3 TRCN0000007538 CCCTGATGAACTAAGATTTAA pLKO.1 979 CDS 100% 15.000 10.500 N UCHL3 n/a
4 TRCN0000320682 CCCTGATGAACTAAGATTTAA pLKO_005 979 CDS 100% 15.000 10.500 N UCHL3 n/a
5 TRCN0000007539 GTCTTACTTCTCTTTCCTATT pLKO.1 293 CDS 100% 10.800 7.560 N UCHL3 n/a
6 TRCN0000320753 GTCTTACTTCTCTTTCCTATT pLKO_005 293 CDS 100% 10.800 7.560 N UCHL3 n/a
7 TRCN0000007537 CCTGGAGGAATCTGTGTCAAT pLKO.1 505 CDS 100% 4.950 3.465 N UCHL3 n/a
8 TRCN0000320754 CCTGGAGGAATCTGTGTCAAT pLKO_005 505 CDS 100% 4.950 3.465 N UCHL3 n/a
9 TRCN0000007535 GTCAATAATGGAAACACCAAA pLKO.1 1028 3UTR 100% 4.950 3.465 N UCHL3 n/a
10 TRCN0000320757 CTTGTCAATAATGGAAACACC pLKO_005 1025 CDS 100% 2.640 1.848 N UCHL3 n/a
11 TRCN0000030718 GCATGGTACCAAGACCAGTAT pLKO.1 267 CDS 100% 0.000 0.000 N Uchl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011535214.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01745 pDONR223 100% 65.9% 65.9% None 0_1ins108;367_558del n/a
2 ccsbBroad304_01745 pLX_304 0% 65.9% 65.9% V5 0_1ins108;367_558del n/a
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