Construct: ORF ccsbBroad304_01903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018199.1_s304c1, BRDN0000404739
- Derived from:
- ccsbBroadEn_01903
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BC21 (8349)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01903
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 100% | 100% | |
| 2 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 94.4% | 97.6% | (many diffs) |
| 3 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 92.8% | 99.2% | (many diffs) |
| 4 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 91.5% | 98.4% | (many diffs) |
| 5 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 91.5% | 98.4% | (many diffs) |
| 6 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 91.2% | 98.4% | (many diffs) |
| 7 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 91% | 99.2% | (many diffs) |
| 8 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 91% | 96% | (many diffs) |
| 9 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 90.7% | 99.2% | (many diffs) |
| 10 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 90.7% | 97.6% | (many diffs) |
| 11 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 90.4% | 96.8% | (many diffs) |
| 12 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 90.4% | 96.8% | (many diffs) |
| 13 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 90.2% | 99.2% | (many diffs) |
| 14 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 89.9% | 99.2% | (many diffs) |
| 15 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 89.4% | 99.2% | (many diffs) |
| 16 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 89.4% | 98.4% | (many diffs) |
| 17 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 89.4% | 98.4% | (many diffs) |
| 18 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 88.8% | 91.7% | (many diffs) |
| 19 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 88.6% | 98.4% | (many diffs) |
| 20 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 88.3% | 96% | (many diffs) |
| 21 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 87.5% | 98.4% | (many diffs) |
| 22 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 86.7% | 99.2% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 75.1% | 82.6% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 70.7% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 61.9% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 22.1% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 19.1% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.8% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 16.5% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.9% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 14.4% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.8% | (many diffs) | |
| 33 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 92.5% | 98.4% | (many diffs) |
| 34 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 92.3% | 99.2% | (many diffs) |
| 35 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 92.3% | 99.2% | (many diffs) |
| 36 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 92.3% | 99.2% | (many diffs) |
| 37 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 92.3% | 98.4% | (many diffs) |
| 38 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92% | 99.2% | (many diffs) |
| 39 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92% | 99.2% | (many diffs) |
| 40 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 92% | 98.4% | (many diffs) |
| 41 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 92% | 98.4% | (many diffs) |
| 42 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 92% | 98.4% | (many diffs) |
| 43 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 91.7% | 99.2% | (many diffs) |
| 44 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 91.7% | 98.4% | (many diffs) |
| 45 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 91.7% | 98.4% | (many diffs) |
| 46 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 91.5% | 98.4% | (many diffs) |
| 47 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 91.5% | 97.6% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 91% | 96.8% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 91% | 96.8% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 89.4% | 96% | (many diffs) |
| 51 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 88.3% | 96.8% | (many diffs) |
| 52 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 88.2% | 94.4% | (many diffs) |
| 53 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 87.3% | 97.6% | (many diffs) |
| 54 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 86.3% | 91.7% | (many diffs) |
| 55 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 86.3% | 91.7% | (many diffs) |
| 56 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 82.9% | 88.1% | (many diffs) |
| 57 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 82.8% | 88.4% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 444
- ORF length:
- 378
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgaaccggca aaatccgctc cggcccctaa aaagggctcc aagaaagccg 121 tcaccaaagc ccagaagaaa gacggcaaga agcgcaagcg cagccgcaaa gagagctact 181 ccatctacgt gtacaaggtg ctgaagcagg tccaccccga caccggcatc tcgtccaagg 241 ccatgggcat catgaactcc ttcgtcaacg acatcttcga gcgcatcgcg ggagaggctt 301 cccgcctggc gcactacaac aagcgctcca ccatcacatc ccgcgagatc cagacggccg 361 tgcgcctgct gctgcccggc gagctggcca agcacgccgt gtccgagggc accaaggcgg 421 tcaccaagta caccagctcc aagtgcccaa ctttcttgta caaagtggtt ggtaagccta 481 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 541 gcgttaagtc gacaatcaac ctctggatta