Transcript: Human NM_175055.2

Homo sapiens H2B.U histone 1 (H2BU1), mRNA.

Source:
NCBI, updated 2019-07-26
Taxon:
Homo sapiens (human)
Gene:
H2BU1 (128312)
Length:
452
CDS:
24..404

Additional Resources:

NCBI RefSeq record:
NM_175055.2
NBCI Gene record:
H2BU1 (128312)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_175055.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106664 TGCGCCCAAGAAGGGTTCTAA pLKO.1 50 CDS 100% 5.625 7.875 N H2BU1 n/a
2 TRCN0000106660 GCGCCCAAGAAGGGTTCTAAA pLKO.1 51 CDS 100% 13.200 9.240 N H2BU1 n/a
3 TRCN0000106661 CGCGAAGTGCAGACGGCCGTT pLKO.1 300 CDS 100% 0.000 0.000 N H2BU1 n/a
4 TRCN0000433481 ACAGAAGAAGGACGGCAAGAA pLKO_005 89 CDS 100% 4.950 2.970 N H2BU1 n/a
5 TRCN0000106663 GAGCTATTCTATCTACGTGTA pLKO.1 131 CDS 100% 4.050 2.430 N H2BU1 n/a
6 TRCN0000437622 CACACTACAACAAGCGCTCCA pLKO_005 268 CDS 100% 2.160 1.296 N H2BU1 n/a
7 TRCN0000106662 CCGCAAGGAGAGCTATTCTAT pLKO.1 122 CDS 100% 5.625 2.813 Y H2BU1 n/a
8 TRCN0000096962 CAAGGCCATGGGCATCATGAA pLKO.1 194 CDS 100% 4.950 2.475 Y Hist1h2bn n/a
9 TRCN0000435259 CAGAAGAAGGACGGCAAGAAG pLKO_005 90 CDS 100% 4.950 2.475 Y Hist1h2bg n/a
10 TRCN0000096948 CCAAGGCTGTCACCAAGTACA pLKO.1 370 CDS 100% 4.950 2.475 Y Hist1h2be n/a
11 TRCN0000441789 ACGTGTACAAGGTGCTGAAGC pLKO_005 145 CDS 100% 4.050 2.025 Y H2BC13 n/a
12 TRCN0000438590 CAAGGCTGTCACCAAGTACAC pLKO_005 371 CDS 100% 4.050 2.025 Y H2BU1 n/a
13 TRCN0000106676 CATCATGAACTCCTTCGTCAA pLKO.1 206 CDS 100% 4.050 2.025 Y H2BC15 n/a
14 TRCN0000106724 CTACGTGTACAAGGTGCTGAA pLKO.1 143 CDS 100% 4.050 2.025 Y H2BC21 n/a
15 TRCN0000256092 TACAACAAGCGCTCCACCATC pLKO_005 273 CDS 100% 4.050 2.025 Y H2BC7 n/a
16 TRCN0000375583 TACGTGTACAAGGTGCTGAAG pLKO_005 144 CDS 100% 4.050 2.025 Y Hist1h2bb n/a
17 TRCN0000425656 TACGTGTACAAGGTGCTGAAG pLKO_005 144 CDS 100% 4.050 2.025 Y H2BC15 n/a
18 TRCN0000096945 CACCAAGTACACCAGCTCCAA pLKO.1 380 CDS 100% 2.640 1.320 Y Hist1h2be n/a
19 TRCN0000106998 CATGGGCATCATGAACTCCTT pLKO.1 200 CDS 100% 2.640 1.320 Y H2BC7 n/a
20 TRCN0000086412 CAATGACATCTTCGAGCGCAT pLKO.1 224 CDS 100% 2.160 1.080 Y Hist3h2bb-ps n/a
21 TRCN0000096973 GAAGGACGGCAAGAAGCGCAA pLKO.1 95 CDS 100% 0.720 0.360 Y Hist1h2bl n/a
22 TRCN0000431230 ATCATGAACTCCTTCGTCAAC pLKO_005 207 CDS 100% 4.050 2.025 Y H2BC14 n/a
23 TRCN0000431028 TCATGAACTCCTTCGTCAACG pLKO_005 208 CDS 100% 4.050 2.025 Y H2BC13 n/a
24 TRCN0000106719 ACCAAGGCACAGAAGAAGGAT pLKO.1 81 CDS 100% 3.000 1.500 Y H2BC10 n/a
25 TRCN0000093135 GTGTACAAGGTGCTGAAGCAA pLKO.1 147 CDS 100% 3.000 1.500 Y Hist1h2bp n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_175055.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04839 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04839 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466986 TCACGGGATGAGCTCCAAAAATGA pLX_317 100% 100% 100% V5 n/a
4 ccsbBroadEn_05671 pDONR223 100% 91.2% 95.2% None (many diffs) n/a
5 ccsbBroad304_05671 pLX_304 0% 91.2% 95.2% V5 (many diffs) n/a
6 TRCN0000476574 TAAAAAAACATGTTATCTATAAGA pLX_317 92.5% 91.2% 95.2% V5 (many diffs) n/a
7 ccsbBroadEn_01903 pDONR223 100% 91% 96% None (many diffs) n/a
8 ccsbBroad304_01903 pLX_304 0% 91% 96% V5 (many diffs) n/a
9 TRCN0000474598 ACTACAGCTCACCGGCGTAGGAAC pLX_317 100% 91% 96% V5 (many diffs) n/a
10 ccsbBroadEn_07224 pDONR223 100% 90.4% 96% None (many diffs) n/a
11 ccsbBroad304_07224 pLX_304 0% 90.4% 96% V5 (many diffs) n/a
12 TRCN0000472239 ATGAGCATATTTAAATGCTTTCTC pLX_317 100% 90.4% 96% V5 (many diffs) n/a
13 ccsbBroadEn_01902 pDONR223 100% 89.6% 96.8% None (many diffs) n/a
14 ccsbBroad304_01902 pLX_304 0% 89.6% 96.8% V5 (many diffs) n/a
15 TRCN0000473896 CTGGCTTAAGACAGAATAAACCCC pLX_317 100% 89.6% 96.8% V5 (many diffs) n/a
16 ccsbBroadEn_01898 pDONR223 100% 89.1% 96% None (many diffs) n/a
17 ccsbBroad304_01898 pLX_304 0% 89.1% 96% V5 (many diffs) n/a
18 TRCN0000469156 CCCGCACAGGGATTAGCAAAGTTG pLX_317 89.9% 89.1% 96% V5 (many diffs) n/a
19 ccsbBroadEn_01900 pDONR223 100% 88.3% 95.2% None (many diffs) n/a
20 ccsbBroad304_01900 pLX_304 0% 88.3% 95.2% V5 (many diffs) n/a
21 TRCN0000470158 AGTTTACTCTTCCCTTTATTAGAT pLX_317 90.5% 88.3% 95.2% V5 (many diffs) n/a
22 ccsbBroadEn_04472 pDONR223 100% 87.8% 94.4% None (many diffs) n/a
23 ccsbBroad304_04472 pLX_304 0% 87.8% 94.4% V5 (many diffs) n/a
24 TRCN0000471184 TCCCCACAGAAAAAGCTTCGCGAC pLX_317 82.9% 87.8% 94.4% V5 (many diffs) n/a
25 ccsbBroadEn_15861 pDONR223 0% 87.5% 93.6% None (many diffs) n/a
26 ccsbBroad304_15861 pLX_304 0% 87.5% 93.6% V5 (many diffs) n/a
27 TRCN0000466058 TATAACTAACAAACAAGCTAACTT pLX_317 98.5% 87.5% 93.6% V5 (many diffs) n/a
28 ccsbBroadEn_12031 pDONR223 100% 87.3% 92.8% None (many diffs) n/a
29 ccsbBroad304_12031 pLX_304 0% 87.3% 92.8% V5 (many diffs) n/a
30 TRCN0000469135 AACATACCTTGTTGTCATTTATTA pLX_317 100% 87.3% 92.8% V5 (many diffs) n/a
31 ccsbBroadEn_06348 pDONR223 100% 87.3% 95.2% None (many diffs) n/a
32 ccsbBroad304_06348 pLX_304 0% 87.3% 95.2% V5 (many diffs) n/a
33 TRCN0000466554 GCTTTTTGTCTTCCCTACTCACGT pLX_317 98.5% 87.3% 95.2% V5 (many diffs) n/a
34 ccsbBroadEn_07223 pDONR223 100% 86.5% 95.2% None (many diffs) n/a
35 ccsbBroad304_07223 pLX_304 0% 86.5% 95.2% V5 (many diffs) n/a
36 TRCN0000471318 CGGCGACCCACTCTTCACCGCCCG pLX_317 96.2% 86.5% 95.2% V5 (many diffs) n/a
37 ccsbBroadEn_01901 pDONR223 100% 85.7% 96% None (many diffs) n/a
38 ccsbBroad304_01901 pLX_304 0% 85.7% 96% V5 (many diffs) n/a
39 TRCN0000468700 CGACGATGACTCTCCCTCATTTGA pLX_317 100% 85.7% 96% V5 (many diffs) n/a
40 ccsbBroadEn_01897 pDONR223 100% 85.4% 95.2% None (many diffs) n/a
41 ccsbBroad304_01897 pLX_304 0% 85.4% 95.2% V5 (many diffs) n/a
42 TRCN0000471861 TGCACGGGACGCACCAAATGACCA pLX_317 90.4% 85.4% 95.2% V5 (many diffs) n/a
43 ccsbBroadEn_01899 pDONR223 100% 84.9% 92.8% None (many diffs) n/a
44 ccsbBroad304_01899 pLX_304 0% 84.9% 92.8% V5 (many diffs) n/a
45 TRCN0000477535 GAGTATGATTCTCGACACACTATA pLX_317 98.5% 84.9% 92.8% V5 (many diffs) n/a
46 ccsbBroadEn_11267 pDONR223 100% 67.6% 72.8% None (many diffs) n/a
47 ccsbBroad304_11267 pLX_304 0% 67.6% 72.8% V5 (many diffs) n/a
48 TRCN0000469107 ATTTGGTTCAGATACTGGTGTTTT pLX_317 18.5% 67.6% 72.8% V5 (many diffs) n/a
49 ccsbBroadEn_11322 pDONR223 100% 53% 57.1% None (many diffs) n/a
50 ccsbBroad304_11322 pLX_304 0% 53% 57.1% V5 (many diffs) n/a
51 TRCN0000471205 GAGTACAAGAAACTGCTGAAATGG pLX_317 100% 53% 57.1% V5 (many diffs) n/a
52 ccsbBroadEn_10354 pDONR223 100% 48.4% 50% None (many diffs) n/a
53 ccsbBroad304_10354 pLX_304 0% 48.4% 50% V5 (many diffs) n/a
54 TRCN0000466355 TTCTAACCCCTTTGTAGACCAATG pLX_317 100% 48.4% 50% V5 (many diffs) n/a
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