Construct: ORF ccsbBroad304_01905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006194.1_s304c1, BRDN0000411622
- Derived from:
- ccsbBroadEn_01905
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3C3 (8352)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01905
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 100% | 100% | |
| 2 | human | 8353 | H3C6 | H3 clustered histone 6 | NM_003532.2 | 87.2% | 100% | (many diffs) |
| 3 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 87% | 100% | (many diffs) |
| 4 | human | 8358 | H3C2 | H3 clustered histone 2 | NM_003537.3 | 85% | 100% | (many diffs) |
| 5 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 84.8% | 100% | (many diffs) |
| 6 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 84.3% | 100% | (many diffs) |
| 7 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 84.3% | 99.2% | (many diffs) |
| 8 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 84.3% | 99.2% | (many diffs) |
| 9 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 84% | 99.2% | (many diffs) |
| 10 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 83.5% | 100% | (many diffs) |
| 11 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 83.5% | 100% | (many diffs) |
| 12 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 83.3% | 97% | (many diffs) |
| 13 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 83.3% | 97% | (many diffs) |
| 14 | human | 8350 | H3C1 | H3 clustered histone 1 | NM_003529.2 | 83% | 100% | (many diffs) |
| 15 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 83% | 100% | (many diffs) |
| 16 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 82.1% | 97% | (many diffs) |
| 17 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 79.7% | 96.3% | (many diffs) |
| 18 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 77.4% | 96.3% | (many diffs) |
| 19 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 76.9% | 93.3% | (many diffs) |
| 20 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 87.5% | 100% | (many diffs) |
| 21 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 87.2% | 100% | (many diffs) |
| 22 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 87.2% | 100% | (many diffs) |
| 23 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 87.2% | 100% | (many diffs) |
| 24 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 86.7% | 99.2% | (many diffs) |
| 25 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 86.7% | 99.2% | (many diffs) |
| 26 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 86.5% | 99.2% | (many diffs) |
| 27 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 86.5% | 99.2% | (many diffs) |
| 28 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 86.2% | 99.2% | (many diffs) |
| 29 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 86.2% | 99.2% | (many diffs) |
| 30 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 86.2% | 99.2% | (many diffs) |
| 31 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 86% | 99.2% | (many diffs) |
| 32 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 84.8% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcgtacg aagcaaacag ctcgcaagtc taccggcggc aaagctccgc 121 gcaagcagct tgctactaaa gcagcccgta agagcgctcc ggccaccggt ggcgtgaaga 181 aacctcatcg ctaccgcccg ggcaccgtgg ccttgcgcga aatccgtcgc taccagaagt 241 ccaccgagct gctgatccgg aagctgccgt tccagcgcct ggtgcgagaa atcgcccagg 301 acttcaaaac cgacctgcgt ttccagagct ctgcggtgat ggcgctgcag gaggcttgtg 361 aggcctacct ggtgggactc ttcgaagaca ccaatctgtg cgctattcac gctaaacgcg 421 tcaccatcat gcccaaagat atccagctgg cacgtcgcat ccgtggggaa agggcattgc 481 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 541 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 601 tta