Construct: ORF ccsbBroad304_01960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005583.1_s304c1, BRDN0000408325
- Derived from:
- ccsbBroadEn_01960
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASK (8573)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01960
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_001126054.2 | 100% | 100% | |
| 2 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_001126055.2 | 98.7% | 98.7% | 1015_1016insGACTTCTAGCAGCAGAAA;2067_2081del |
| 3 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543995.2 | 98.1% | 98% | 173_190del;1033_1034insGACTTCTAGCAGCAGAAA;2085_2099del |
| 4 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_003688.3 | 97.5% | 97.5% | 1737_1805del |
| 5 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_006724566.3 | 97.4% | 97.4% | 1015_1016insGACTTCTAGCAGCAGAAA;1717_1718ins36;2031_2045del |
| 6 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_001367721.1 | 96.9% | 96.9% | 1737_1805del;2154_2168del |
| 7 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543996.2 | 96.8% | 96.6% | (many diffs) |
| 8 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543994.2 | 96.5% | 95.9% | (many diffs) |
| 9 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_005272686.4 | 96.3% | 96.3% | 1015_1016insGACTTCTAGCAGCAGAAA;1719_1787del;2136_2150del |
| 10 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543993.2 | 95.7% | 95.6% | (many diffs) |
| 11 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_024452473.1 | 76.9% | 76.9% | (many diffs) |
| 12 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543997.3 | 76.3% | 76.3% | (many diffs) |
| 13 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_006527561.3 | 95.1% | 99.8% | (many diffs) |
| 14 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318370.1 | 94.5% | 99.1% | (many diffs) |
| 15 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318369.1 | 93.9% | 98.5% | (many diffs) |
| 16 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_009806.3 | 93.9% | 98.6% | (many diffs) |
| 17 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247440.2 | 93.5% | 97.7% | (many diffs) |
| 18 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247441.2 | 93.3% | 97.9% | (many diffs) |
| 19 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_001284504.1 | 93.1% | 97.8% | (many diffs) |
| 20 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318368.1 | 92.9% | 97% | (many diffs) |
| 21 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_006527560.3 | 92.7% | 97.3% | (many diffs) |
| 22 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318371.1 | 92.7% | 97.2% | (many diffs) |
| 23 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247439.2 | 92.4% | 96.5% | (many diffs) |
| 24 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_001284503.1 | 92.2% | 96.8% | (many diffs) |
| 25 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318367.1 | 92.1% | 96.6% | (many diffs) |
| 26 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247438.2 | 91.6% | 96.1% | (many diffs) |
| 27 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_001284505.1 | 91.6% | 96.2% | (many diffs) |
| 28 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247436.2 | 90% | 94.4% | (many diffs) |
| 29 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318366.1 | 89.3% | 93.7% | (many diffs) |
| 30 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247437.1 | 88.7% | 93.1% | (many diffs) |
| 31 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247435.2 | 88.6% | 92.5% | (many diffs) |
| 32 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247433.1 | 88.4% | 92.8% | (many diffs) |
| 33 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247432.1 | 88.3% | 92.6% | (many diffs) |
| 34 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247431.1 | 87.8% | 92.1% | (many diffs) |
| 35 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247434.1 | 87.2% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2763
- ORF length:
- 2694
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgacgac gacgtgctgt tcgaggatgt gtacgagctg tgcgaggtga 121 tcggaaaggg tcccttcagt gttgtacgac gatgtatcaa cagagaaact gggcaacaat 181 ttgctgtaaa aattgttgat gtagccaagt tcacatcaag tccagggtta agtacagaag 241 atctaaagcg ggaagccagt atctgtcata tgctgaaaca tccacacatt gtagagttat 301 tggagacata tagctcagat ggaatgcttt acatggtttt cgaatttatg gatggagcag 361 atctgtgttt tgaaatcgta aagcgagctg acgctggttt tgtgtacagt gaagctgtag 421 ccagccatta tatgagacag atactggaag ctctacgcta ctgccatgat aataacataa 481 ttcacaggga tgtgaagccc cactgtgttc tccttgcctc aaaagaaaac tcggcacctg 541 ttaaacttgg aggctttggg gtagctattc aattagggga gtctggactt gtagctggag 601 gacgtgttgg aacacctcat tttatggcac cagaagtggt caaaagagag ccttacggaa 661 agcctgtaga cgtctggggg tgcggtgtga tcctttttat cctgctcagt ggttgtttgc 721 ctttttacgg aaccaaggaa agattgtttg aaggcattat taaaggaaaa tataagatga 781 atccaaggca gtggagccat atctctgaaa gtgccaaaga cctagtacgt cgcatgctga 841 tgctggatcc agctgaaagg atcactgttt atgaagcact gaatcaccca tggcttaagg 901 agcgggatcg ttacgcctac aagattcatc ttccagaaac agtagagcag ctgaggaaat 961 tcaatgcaag gaggaaacta aagggtgcag tactagccgc tgtgtcaagt cacaaattca 1021 actcattcta tggggatccc cctgaagagt taccagattt ctccgaagac cctacctcct 1081 caggacttct agcagcagaa agagcagtct cacaggtgct ggacagcctg gaagagattc 1141 atgcgcttac agactgcagt gaaaaggacc tagattttct acacagtgtt ttccaggatc 1201 agcatcttca cacactacta gatctgtatg acaaaattaa cacaaagtct tcaccacaaa 1261 tcaggaatcc tccaagcgat gcagtacaga gagccaaaga ggtattggaa gaaatttcat 1321 gttaccctga gaataacgac gcaaaggaac taaagcgtat tttaacacaa cctcatttca 1381 tggccttact tcagactcac gacgtagtgg cacatgaagt ttacagtgat gaagcattga 1441 gggtcacacc tcctcccacc tctccctatt taaacggcga ttctccagaa agtgctaacg 1501 gagacatgga tatggagaat gtgaccagag ttcggctggt acagtttcaa aagaacacag 1561 atgaaccaat gggaatcact ttaaaaatga atgaactaaa tcattgtatt gttgcaagaa 1621 ttatgcatgg gggcatgatt cacaggcaag gtacacttca tgttggtgat gaaattcgag 1681 aaatcaatgg catcagtgtg gctaaccaaa cagtggaaca actgcaaaaa atgcttaggg 1741 aaatgcgggg gagtattacc ttcaagattg tgccaagtta ccgcactcag tcttcgtcct 1801 gtgaggactt gccatcaact acccaaccaa aaggacgaca gatctatgta agagcacaat 1861 ttgaatatga tccagccaag gatgacctca tcccctgtaa agaagctggc attcgattca 1921 gagttggtga catcatccag attattagta aggatgatca taattggtgg cagggtaaac 1981 tggaaaactc caaaaatgga actgcaggtc tcattccttc tcctgaactt caggaatggc 2041 gagtagcttg cattgccatg gagaagacca aacaggagca gcaggccagc tgtacttggt 2101 ttggcaagaa aaagaagcag tacaaagata aatatttggc aaagcacaat gcagatcttg 2161 tcacatatga agaagtagta aaactgccag cattcaagag gaaaacacta gtcttattag 2221 gcgcacatgg tgttgggaga agacacataa aaaacactct catcacaaag cacccagacc 2281 ggtttgcgta ccctattcca catacaacca gacctccaaa gaaagacgaa gaaaatggaa 2341 agaattatta ctttgtatct catgaccaaa tgatgcaaga catctctaat aacgagtact 2401 tggagtacgg cagccacgag gatgcgatgt atgggacaaa actggagacc atccggaaga 2461 tccacgagca ggggctgatt gcaatactgg acgtggagcc tcaggcactg aaggtcctga 2521 gaactgcaga gtttgctcct tttgttgttt tcattgctgc accaactatt actccaggtt 2581 taaatgagga tgaatctctt cagcgtctgc agaaggagtc tgacatctta cagagaacat 2641 atgcacacta cttcgatctc acaattatca acaatgaaat tgatgagaca atcagacatc 2701 tggaggaagc tgttgagctc gtgtgcacag ccccacagtg ggtccctgtc tcctgggtct 2761 atttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2821 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2881 tctggatta