Construct: ORF ccsbBroad304_02032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001095.1_s304c1, BRDN0000402730
- Derived from:
- ccsbBroadEn_02032
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSC22D1 (8848)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02032
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8848 | TSC22D1 | TSC22 domain family member 1 | NM_006022.4 | 100% | 100% | |
| 2 | human | 8848 | TSC22D1 | TSC22 domain family member 1 | NM_001243797.1 | 59.7% | 59.7% | 0_1ins174 |
| 3 | human | 8848 | TSC22D1 | TSC22 domain family member 1 | NM_001243798.1 | 59.7% | 59.7% | 0_1ins174 |
| 4 | human | 8848 | TSC22D1 | TSC22 domain family member 1 | NM_183422.4 | 12.1% | 9.9% | (many diffs) |
| 5 | human | 8848 | TSC22D1 | TSC22 domain family member 1 | XM_024449427.1 | 12.1% | 9.9% | (many diffs) |
| 6 | mouse | 21807 | Tsc22d1 | TSC22 domain family, member 1 | NM_009366.4 | 92.3% | 98.6% | (many diffs) |
| 7 | mouse | 21807 | Tsc22d1 | TSC22 domain family, member 1 | NM_001177751.2 | 67.8% | 70.1% | (many diffs) |
| 8 | mouse | 21807 | Tsc22d1 | TSC22 domain family, member 1 | NM_001310647.1 | 54.8% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa atcccaatgg tgtagaccag tggcgatgga tctaggagtt taccaactga 121 gacatttttc aatttctttc ttgtcatcct tgctggggac tgaaaacgct tctgtgagac 181 ttgataatag ctcctctggt gcaagtgtgg tagctattga caacaaaatc gagcaagcta 241 tggatctagt gaaaagccat ttgatgtatg cggtcagaga agaagtggag gtcctcaaag 301 agcaaatcaa agaactaata gagaaaaatt cccagctgga gcaggagaac aatctgctga 361 agacactggc cagtcctgag cagcttgccc agtttcaggc ccagctgcag actggctccc 421 cccctgccac cacccagcca cagggcacca cacagccccc cgcccagcca gcatcgcagg 481 gctcaggacc aaccgcatac ccaactttct tgtacaaagt ggttggtaag cctatcccta 541 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 601 agtcgacaat caacctctgg atta