Construct: ORF ccsbBroad304_02116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005463.1_s304c1, BRDN0000410269
- Derived from:
- ccsbBroadEn_02116
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBRG4 (9238)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02116
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9238 | TBRG4 | transforming growth factor ... | NM_004749.4 | 100% | 100% | |
| 2 | human | 9238 | TBRG4 | transforming growth factor ... | NM_001261834.1 | 98.2% | 98.2% | 1_33del |
| 3 | human | 9238 | TBRG4 | transforming growth factor ... | NM_030900.3 | 82.5% | 82.5% | 733_734ins330 |
| 4 | human | 9238 | TBRG4 | transforming growth factor ... | NM_199122.2 | 82.5% | 82.5% | 733_734ins330 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1959
- ORF length:
- 1893
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agctcacctg gtaaagcgat gcacgtgcct cctgagagaa gctgctcgtc 121 aggcccctgc catggctcca gttggccgac tgagacttgc ctgggtagcc cataagactc 181 tgacttcctc agccacctca cccatttccc acctcccagg ttccttgatg gagccggtgg 241 agaaggaacg agcatctact ccctacatag agaagcaggt ggaccacctc atcaagaagg 301 ccacaaggcc agaggagctc ctggagctac ttggtggcag tcacgacttg gacagcaatc 361 aagcagcaat ggtacttatc cggctctctc acttgctgtc tgagaagcca gaagataaag 421 gcttgctcat acaggatgcc cactttcatc aacttctctg tctgctcaac agtcagattg 481 cctcggtctg gcatggtacc ctctcgaagc tgctgggaag cctgtatgct ctgggcatcc 541 ccaaggcctc caaggagctg cagtcggtgg agcaggaggt ccgctggcgc atgcggaagc 601 tcaagtacaa gcacctggcc ttcctggcag agtcctgtgc caccctctca caggagcagc 661 actcgcagga gctgctggct gagctgctca cacacctgga aaggcgttgg acagaaattg 721 aagattccca cacattagtg accgtcatga tgaaggtggg acacctctcg gagccactaa 781 tgaaccgcct ggaagacaag tgcctggagt tggtggagca ctttggcccc aatgagctgc 841 ggaaggtgct ggtgatgctg gcagctcaga gccggcggtc cgtgcccttg ctgcgggcca 901 tctcctacca cctggtgcag aagcccttct ctctgacgaa agatgtgctc ttggacgtgg 961 cctatgccta tggcaaactc agctttcacc agacccaggt gtcccagcgc ctggccaccg 1021 acctgctatc cctcatgccc agcctgactt ctggtgaggt ggcccactgt gccaagtcct 1081 tcgccttact caagtggctc agcctgcccc tgtttgaggc ctttgcccag cacgtcctga 1141 acagagcgca ggacatcacc ctgccccacc tgtgcagcgt acttctggct tttgcgcgtc 1201 tgaacttcca tccagaccaa gaggatcagt tcttcagcct ggtacatgag aagctggggt 1261 cagagctgcc aggcctggag ccagccctgc aggtggacct ggtgtgggcc ctgtgtgtgc 1321 tgcagcaggc acgggaagca gagctgcaag ccgtcctcca ccctgaattt cacatccaat 1381 ttctaggggg caagtctcag aaggatcaga acaccttcca gaagctgctc cacatcaacg 1441 ccactgccct gctggagtac cccgagtact cgggtcccct tctgcctgcc tcggctgtgg 1501 cccctgggcc ctcagccctt gacaggaagg tgacccccct gcaaaaggag ctgcaggaga 1561 cgctgaaggg gctgctgggg agcgccgaca agggcagcct cgaggtggcc acgcagtatg 1621 gctgggtgct ggatgctgag gtgctgctgg acagtgacgg cgagtttctg cccgtaaggg 1681 actttgtggc acctcacctt gcccagccaa ctgggagcca gtcaccacct ccagggtcta 1741 agaggctagc gttcttgcgg tgggagttcc ccaacttcaa cagccgaagc aaggacttgc 1801 tgggtcgctt tgttctggcc cggcgacaca tagtggctgc aggcttcctg atagtggacg 1861 tcccattcta tgagtggctg gaactcaagt ctgaatggca gaaaggcgcc tacctcaagg 1921 acaagatgcg caaagcggtg gctgaggagc tggccaagtg cccaactttc ttgtacaaag 1981 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2041 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta