Construct: ORF ccsbBroad304_02143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003102.1_s304c1, BRDN0000406205
- Derived from:
- ccsbBroadEn_02143
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNOT8 (9337)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02143
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301073.2 | 100% | 100% | |
| 2 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_004779.6 | 100% | 100% | |
| 3 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010048.2 | 100% | 100% | |
| 4 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010049.2 | 100% | 100% | |
| 5 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010050.1 | 100% | 100% | |
| 6 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010051.1 | 100% | 100% | |
| 7 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301074.2 | 81.5% | 81.5% | 311_312ins162 |
| 8 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_005268527.2 | 81.5% | 81.5% | 311_312ins162 |
| 9 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010052.1 | 81.5% | 81.5% | 311_312ins162 |
| 10 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010053.1 | 81.5% | 81.5% | 311_312ins162 |
| 11 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010054.2 | 81.5% | 81.5% | 311_312ins162 |
| 12 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010055.2 | 81.5% | 81.5% | 311_312ins162 |
| 13 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010056.1 | 81.5% | 81.5% | 311_312ins162 |
| 14 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301075.2 | 63.6% | 63.6% | 0_1ins318 |
| 15 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301077.2 | 63.6% | 63.6% | 0_1ins318 |
| 16 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301080.2 | 63.6% | 63.6% | 0_1ins318 |
| 17 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301082.1 | 63.6% | 63.6% | 0_1ins318 |
| 18 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301083.1 | 63.6% | 63.6% | 0_1ins318 |
| 19 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_011537706.1 | 63.6% | 63.6% | 0_1ins318 |
| 20 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_011537708.2 | 63.6% | 63.6% | 0_1ins318 |
| 21 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010057.1 | 63.6% | 63.6% | 0_1ins318 |
| 22 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | NM_001301086.2 | 43.8% | 43.8% | 0_1ins492 |
| 23 | human | 9337 | CNOT8 | CCR4-NOT transcription comp... | XM_017010058.2 | 43.8% | 43.8% | 0_1ins492 |
| 24 | mouse | 69125 | Cnot8 | CCR4-NOT transcription comp... | NM_026949.3 | 88.4% | 99.3% | (many diffs) |
| 25 | mouse | 69125 | Cnot8 | CCR4-NOT transcription comp... | XM_006534102.1 | 88.4% | 99.3% | (many diffs) |
| 26 | mouse | 69125 | Cnot8 | CCR4-NOT transcription comp... | XM_006534103.3 | 88.4% | 99.3% | (many diffs) |
| 27 | mouse | 69125 | Cnot8 | CCR4-NOT transcription comp... | XM_006534104.1 | 88.4% | 99.3% | (many diffs) |
| 28 | mouse | 69125 | Cnot8 | CCR4-NOT transcription comp... | XM_006534105.1 | 88.4% | 99.3% | (many diffs) |
| 29 | mouse | 69125 | Cnot8 | CCR4-NOT transcription comp... | XM_006534106.3 | 88.4% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgcagcactt gtggagaata gccaggttat ctgtgaagtg tgggccagta 121 atctagaaga agagatgagg aagatccgag aaatcgtgct cagttacagt tatattgcca 181 tggacacaga atttccaggt gttgtggtgc gaccaattgg tgaatttcgt agttccatag 241 attaccaata tcagcttctg cggtgcaatg ttgacctttt aaaaattatc cagctgggcc 301 ttacattcac aaatgagaag ggagagtatc cttctggaat caatacttgg cagttcaatt 361 tcaaatttaa ccttacagag gacatgtact cccaggattc catagatctc cttgctaact 421 caggactaca gtttcagaag catgaagagg aagggattga cacactgcac tttgcagagc 481 tgcttatgac atcaggagtg gttctctgtg acaatgtcaa atggctttca tttcatagtg 541 gctatgattt tggctatatg gtaaagttgc ttacagattc tcgtttgcca gaagaggaac 601 atgaattctt tcatattctg aaccttttct tcccatccat ttatgatgtg aaatacctga 661 tgaagagctg caaaaatctt aagggaggtc ttcaggaagt tgctgatcag ttggatttgc 721 agaggattgg aaggcagcac caggcaggct cagactcact gctgacagga atggctttct 781 ttaggatgaa agagttgttt tttgaggaca gcattgatga tgccaagtac tgtgggcggc 841 tctatggctt aggcacagga gtggcccaga agcagaatga ggatgtggac tctgcccagg 901 agaagatgag catcctggcg attatcaaca acatgcagca gtgcccaact ttcttgtaca 961 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1021 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta