Construct: ORF ccsbBroad304_02191
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005430.1_s304c1, BRDN0000407453
- Derived from:
- ccsbBroadEn_02191
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EI24 (9538)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02191
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9538 | EI24 | EI24 autophagy associated t... | NM_004879.5 | 100% | 100% | |
| 2 | human | 9538 | EI24 | EI24 autophagy associated t... | XM_011543069.2 | 100% | 100% | |
| 3 | human | 9538 | EI24 | EI24 autophagy associated t... | NM_001290135.2 | 95.8% | 95.8% | 42_43ins42 |
| 4 | human | 9538 | EI24 | EI24 autophagy associated t... | NM_001330419.2 | 77% | 66.2% | 671_672ins112;786_787ins122 |
| 5 | human | 9538 | EI24 | EI24 autophagy associated t... | XM_011543070.1 | 77% | 66.2% | 671_672ins112;786_787ins122 |
| 6 | human | 9538 | EI24 | EI24 autophagy associated t... | NR_110770.2 | 41.2% | (many diffs) | |
| 7 | human | 9538 | EI24 | EI24 autophagy associated t... | NR_110769.2 | 39.8% | 1_167del;209_210ins146;1042_2045del | |
| 8 | mouse | 13663 | Ei24 | etoposide induced 2.4 mRNA | NM_001199494.1 | 87.8% | 93.2% | (many diffs) |
| 9 | mouse | 13663 | Ei24 | etoposide induced 2.4 mRNA | NM_007915.5 | 87.8% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgacagtgtc aaaacctttc tccaggacct tgccagagga atcaaagact 121 ccatctgggg tatttgtacc atctcaaagc tagatgctcg aatccagcaa aagagagagg 181 agcagcgtcg aagaagggca agtagtgtct tggcacagag aagagcccag agtatagagc 241 ggaagcaaga gagtgagcca cgtattgtta gtagaatttt ccagtgttgt gcttggaatg 301 gtggagtgtt ctggttcagt ctcctcttgt tttatcgagt atttattcct gtgcttcagt 361 cggtaacagc ccgaattatc ggtgacccat cactacatgg agatgtttgg tcgtggctgg 421 aattcttcct cacgtcaatt ttcagtgctc tttgggtgct ccccttgttt gtgcttagca 481 aagtggtgaa tgccatttgg tttcaggata tagctgacct ggcatttgag gtatcaggga 541 ggaagcctca cccattccct agtgtcagca aaataattgc tgacatgctc ttcaaccttt 601 tgctgcaggc tcttttcctc attcagggaa tgtttgtgag tctctttccc atccatcttg 661 tcggtcagct ggttagtctc ctgcatatgt cccttctcta ctcactgtac tgctttgaat 721 atcgttggtt caataaagga attgaaatgc accagcggtt gtctaacata gaaaggaatt 781 ggccttacta ctttgggttt ggtttgccct tggcttttct cacagcaatg cagtcctcat 841 atattatcag tggctgcctt ttctctatcc tctttccttt attcattatc agcgccaatg 901 aagcaaagac ccctggcaaa gcatatctct tccagttgcg cctcttctcc ttggtggtct 961 tcttaagcaa cagactcttc cacaagacag tctacctgca gtcggccctg agcagctcta 1021 cttctgcaga gaagttccct tcaccgcatc cgtcgcctgc caaactgaag gctactgcag 1081 gtcactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1141 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1201 acctctggat ta