Construct: ORF ccsbBroad304_02212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010745.1_s304c1, BRDN0000398755
- Derived from:
- ccsbBroadEn_02212
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF7 (9616)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02212
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9616 | RNF7 | ring finger protein 7 | NM_014245.5 | 100% | 100% | |
| 2 | human | 9616 | RNF7 | ring finger protein 7 | NM_001201370.1 | 85.8% | 85.8% | 174_175ins48 |
| 3 | human | 9616 | RNF7 | ring finger protein 7 | NM_183237.2 | 79.6% | 51.5% | 167_168insGGTGATG;270_271ins62 |
| 4 | human | 9616 | RNF7 | ring finger protein 7 | NR_037702.1 | 14.9% | 1_133del;309_572del;737_2274del | |
| 5 | human | 9616 | RNF7 | ring finger protein 7 | NR_037703.1 | 14.8% | (many diffs) | |
| 6 | mouse | 19823 | Rnf7 | ring finger protein 7 | NM_011279.3 | 93.8% | 96.4% | (many diffs) |
| 7 | mouse | 19823 | Rnf7 | ring finger protein 7 | NM_001311135.1 | 74% | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgacgtggaa gacggagagg aaacctgcgc cctggcctct cactccggga 121 gctcaggctc caagtcggga ggcgacaaga tgttctccct caagaagtgg aacgcggtgg 181 ccatgtggag ctgggacgtg gagtgcgata cgtgcgccat ctgcagggtc caggtgatgg 241 atgcctgtct tagatgtcaa gctgaaaaca aacaagagga ctgtgttgtg gtctggggag 301 aatgtaatca ttccttccac aactgctgca tgtccctgtg ggtgaaacag aacaatcgct 361 gccctctctg ccagcaggac tgggtggtcc aaagaatcgg caaatgccca actttcttgt 421 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 481 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a