Construct: ORF ccsbBroad304_02286

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018404.1_s304c1, BRDN0000401401
Derived from:
ccsbBroadEn_02286
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACOT8 (10005)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02286
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10005 ACOT8 acyl-CoA thioesterase 8 NM_005469.4 100% 100%
2 human 10005 ACOT8 acyl-CoA thioesterase 8 XM_005260239.3 83.3% 73.5% 0_1ins25;100_101ins134
3 human 10005 ACOT8 acyl-CoA thioesterase 8 XR_244130.4 78.5% 1_90del;736_786del;1099_1218del
4 human 10005 ACOT8 acyl-CoA thioesterase 8 XR_001754105.1 67.5% (many diffs)
5 human 10005 ACOT8 acyl-CoA thioesterase 8 XM_011528480.2 57.6% 57.6% 0_1ins405
6 mouse 170789 Acot8 acyl-CoA thioesterase 8 NM_133240.2 84.3% 84.6% (many diffs)
7 mouse 170789 Acot8 acyl-CoA thioesterase 8 XM_011239319.2 62.4% 62.6% (many diffs)
8 mouse 170789 Acot8 acyl-CoA thioesterase 8 XM_006498831.3 50.5% 51% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1026
ORF length:
957
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcgtccccg caggccccag aagatgggca gggctgtggc gaccgcggcg
121atccccctgg ggacctccgt agcgtcttgg tcacgaccgt gctcaacctc gagccgctgg
181acgaggatct cttcagagga aggcattact gggtaccggc caagaggctg tttggtggtc
241agatcgtggg ccaggccctg gtggctgcag ccaagtctgt gagtgaagac gtccacgtgc
301actccctgca ctgctacttt gttcgggcag gggacccgaa gctgccagta ctgtaccaag
361tggagcggac acgaacaggg tcgagcttct cggtgcgctc tgtgaaggcc gtgcaacatg
421ggaagcccat cttcatctgc caggcctcct tccagcaggc ccagcccagc cccatgcagc
481accagttctc catgcccact gtgccaccac cagaagagct gcttgactgt gagaccctca
541ttgaccagta tttaagggac cctaacctcc aaaagaggta cccattggcg ctcaaccgaa
601ttgctgctca ggaggtcccc attgagatca agccagtaaa cccatccccc ctgagccagc
661tgcagagaat ggagcccaaa cagatgttct gggtgcgagc ccggggctat attggcgagg
721gcgacatgaa gatgcactgc tgcgtggccg cctatatctc cgactatgcc ttcttgggca
781ctgcactgct gcctcaccag tggcagcaca aggtgcactt catggtctca ctggaccatt
841ccatgtggtt ccacgccccc ttccgagctg accactggat gctctatgaa tgcgagagcc
901cctgggccgg tggctctcgg gggctggtcc atgggcggct gtggcgtcag gatggagtcc
961tagctgtgac ctgtgcccag gagggcgtga tccgagtgaa gccccaggtc tcagagagca
1021agctgttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1081gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1141acctctggat ta

Download FASTA (ORF) (Full)