Construct: ORF ccsbBroad304_02323

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011551.1_s304c1, BRDN0000400047
Derived from:
ccsbBroadEn_02323
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDIA6 (10130)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02323
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10130 PDIA6 protein disulfide isomerase... NM_005742.4 100% 100%
2 human 10130 PDIA6 protein disulfide isomerase... NM_001282707.1 99.1% 98.8% (many diffs)
3 human 10130 PDIA6 protein disulfide isomerase... NM_001282706.1 97.7% 97.7% (many diffs)
4 human 10130 PDIA6 protein disulfide isomerase... XM_011510308.2 91.3% 88.4% 1155_1156ins97;1206_1207insTAGGGAAAGATGAGTTG
5 human 10130 PDIA6 protein disulfide isomerase... NM_001282705.2 89.1% 89.1% (many diffs)
6 human 10130 PDIA6 protein disulfide isomerase... NM_001282704.1 88.4% 88.4% (many diffs)
7 mouse 71853 Pdia6 protein disulfide isomerase... NM_027959.3 86% 94.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1386
ORF length:
1320
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tctcctggtg ctcggtctgg tgagctgtac cttctttctg gcagtgaatg
121gtctgtattc ctctagtgat gatgtgatcg aattaactcc atcgaatttc aaccgagaag
181ttattcagag tgatagtttg tggcttgtag aattctatgc tccatggtgt ggtcactgtc
241aaagattaac accagaatgg aagaaagcag caactgcatt aaaagatgtt gtcaaagttg
301gtgcagttga tgcagataag catcattccc taggaggtca gtatggtgtt cagggatttc
361ctaccattaa gatttttgga tccaacaaaa acagaccaga agattaccaa ggtggcagaa
421ctggtgaagc cattgtagat gctgcgctga gtgctctgcg ccagctcgtg aaggatcgcc
481tcgggggacg gagcggagga tacagttctg gaaaacaagg cagaagtgat agttcaagta
541agaaggatgt gattgagctg acagacgaca gctttgataa gaatgttctg gacagtgaag
601atgtttggat ggttgagttc tatgctcctt ggtgtggaca ctgcaaaaac ctagagccag
661agtgggctgc cgcagcttca gaagtaaaag agcagacgaa aggaaaagtg aaactggcag
721ctgtggatgc tacagtcaat caggttctgg cctcccgata cgggattaga ggatttccta
781caatcaagat atttcagaaa ggcgagtctc ctgtggatta tgacggtggg cggacaagat
841ccgacatcgt gtcccgggcc cttgatttgt tttctgataa cgccccacct cctgagctgc
901ttgagattat caacgaggac attgccaaga ggacgtgtga ggagcaccag ctctgtgttg
961tggctgtgct gccccatatc cttgatactg gagctgcagg cagaaattct tatctggaag
1021ttcttctgaa gttggcagac aaatacaaaa agaaaatgtg ggggtggctg tggacagaag
1081ctggagccca gtctgaactt gagaccgcgt tggggattgg agggtttggg taccccgcca
1141tggccgccat caatgcacgc aagatgaaat ttgctctgct aaaaggctcc ttcagtgagc
1201aaggcatcaa cgagtttctc agggagctct cttttgggcg tggctccacg gcacctgtag
1261gaggcggggc tttccctacc atcgttgaga gagagccttg ggacggcagg gatggcgagc
1321ttcccgtgga ggatgacatt gacctcagtg atgtggagct tgatgactta gggaaagatg
1381agttgtgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1441gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1501acctctggat ta

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