Transcript: Human NM_001282704.1

Homo sapiens protein disulfide isomerase family A member 6 (PDIA6), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
PDIA6 (10130)
Length:
2700
CDS:
339..1817

Additional Resources:

NCBI RefSeq record:
NM_001282704.1
NBCI Gene record:
PDIA6 (10130)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001282704.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049356 CGATACGGGATTAGAGGATTT pLKO.1 1185 CDS 100% 10.800 15.120 N PDIA6 n/a
2 TRCN0000049354 GAGATTATCAACGAGGACATT pLKO.1 1332 CDS 100% 4.950 6.930 N PDIA6 n/a
3 TRCN0000049353 CCATCGAATTTCAACCGAGAA pLKO.1 588 CDS 100% 4.050 5.670 N PDIA6 n/a
4 TRCN0000429556 GATGAAATTTGCTCTGCTAAA pLKO_005 1592 CDS 100% 10.800 8.640 N PDIA6 n/a
5 TRCN0000433473 AGTTAGTATCCACATCATAAA pLKO_005 2228 3UTR 100% 13.200 9.240 N PDIA6 n/a
6 TRCN0000444319 GCGAGTCTCCTGTGGATTATG pLKO_005 1231 CDS 100% 13.200 9.240 N PDIA6 n/a
7 TRCN0000433401 GTAGTACTTGATTGGTCATTT pLKO_005 1935 3UTR 100% 13.200 9.240 N PDIA6 n/a
8 TRCN0000416687 AGAAGTGATAGTTCAAGTAAG pLKO_005 951 CDS 100% 10.800 7.560 N PDIA6 n/a
9 TRCN0000433707 AGCAAGGCATCAACGAGTTTC pLKO_005 1627 CDS 100% 10.800 7.560 N PDIA6 n/a
10 TRCN0000049355 GCAGATAAGCATCATTCCCTA pLKO.1 741 CDS 100% 2.640 1.848 N PDIA6 n/a
11 TRCN0000049357 CGAGAAGTTATTCAGAGTGAT pLKO.1 603 CDS 100% 4.950 2.970 N PDIA6 n/a
12 TRCN0000111774 CGCAAGATGAAATTTGCTCTT pLKO.1 1587 CDS 100% 4.050 2.835 N Pdia6 n/a
13 TRCN0000349085 CGCAAGATGAAATTTGCTCTT pLKO_005 1587 CDS 100% 4.050 2.835 N Pdia6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001282704.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02323 pDONR223 100% 88.4% 88.4% None (many diffs) n/a
2 ccsbBroad304_02323 pLX_304 0% 88.4% 88.4% V5 (many diffs) n/a
3 TRCN0000470664 TGTACCGAAAAATACCTCCTATGG pLX_317 37.5% 88.4% 88.4% V5 (many diffs) n/a
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