Construct: ORF ccsbBroad304_02324

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010560.1_s304c1, BRDN0000405053
Derived from:
ccsbBroadEn_02324
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRAP1 (10131)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02324
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10131 TRAP1 TNF receptor associated pro... NM_016292.3 100% 100%
2 human 10131 TRAP1 TNF receptor associated pro... NM_001272049.2 92.4% 92.4% 87_88ins159
3 human 10131 TRAP1 TNF receptor associated pro... XM_011522345.2 80.1% 80.1% 0_1ins420
4 mouse 68015 Trap1 TNF receptor-associated pro... NM_026508.2 84.1% 88.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2178
ORF length:
2112
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gcgcgagctg cgggcgctgc tgctgtgggg ccgccgcctg cggcctttgc
121tgcgggcgcc ggcgctggcg gccgtgccgg gaggaaaacc aattctgtgt cctcggagga
181ccacagccca gttgggcccc aggcgaaacc cagcctggag cttgcaggca ggacgactgt
241tcagcacgca gaccgccgag gacaaggagg aacccctgca ctcgattatc agcagcacag
301agagcgtgca gggttccact tccaaacatg agttccaggc cgagacaaag aagcttttgg
361acattgttgc ccggtccctg tactcagaaa aagaggtgtt tatacgggag ctgatctcca
421atgccagcga tgccttggaa aaactgcgtc acaaactggt gtctgacggc caagcactgc
481cagaaatgga gattcacttg cagaccaatg ccgagaaagg caccatcacc atccaggata
541ctggtatcgg gatgacacag gaagagctgg tgtccaacct ggggacgatt gccagatcgg
601ggtcaaaggc cttcctggat gctctgcaga accaggctga ggccagcagc aagatcatcg
661gccagtttgg agtgggtttc tactcagctt tcatggtggc tgacagagtg gaggtctatt
721cccgctcggc agccccgggg agcctgggtt accagtggct ttcagatggt tctggagtgt
781ttgaaatcgc cgaagcttcg ggagttagaa ccgggacaaa aatcatcatc cacctgaaat
841ccgactgcaa ggagttttcc agcgaggccc gggtgcgaga tgtggtaacg aagtacagca
901acttcgtcag cttccccttg tacttgaatg gaaggcggat gaacaccttg caggccatct
961ggatgatgga ccccaaggat gtccgtgagt ggcaacatga ggagttctac cgctacgtcg
1021cgcaggctca cgacaagccc cgctacaccc tgcactataa gacggacgca ccgctcaaca
1081tccgcagcat cttctacgtg cccgacatga aaccgtccat gtttgatgtg agccgggagc
1141tgggctccag cgttgcactg tacagccgca aagtcctcat ccagaccaag gccacggaca
1201tcctgcccaa gtggctgcgc ttcatccgag gtgtggtgga cagtgaggac attcccctga
1261acctcagccg ggagctgctg caggagagcg cactcatcag gaaactccgg gacgttttac
1321agcagaggct gatcaaattc ttcattgacc agagtaaaaa agatgctgag aagtatgcaa
1381agttttttga agattacggc ctgttcatgc gggagggcat tgtgaccgcc accgagcagg
1441aggtcaagga ggacatagca aagctgctgc gctacgagtc ctcggcgctg ccctccgggc
1501agctaaccag cctctcagaa tacgccagcc gcatgcgggc cggcacccgc aacatctact
1561acctgtgcgc ccccaaccgt cacctggcag agcactcacc ctactatgag gccatgaaga
1621agaaagacac agaggttctc ttctgctttg agcagtttga tgagctcacc ctgctgcacc
1681ttcgtgagtt tgacaagaag aagctgatct ctgtggagac ggacatagtc gtggatcact
1741acaaggagga gaagtttgag gacaggtccc cagccgccga gtgcctatca gagaaggaga
1801cggaggagct catggcctgg atgagaaatg tgctggggtc gcgtgtcacc aacgtgaagg
1861tgaccctccg actggacacc caccctgcca tggtcaccgt gctggagatg ggggctgccc
1921gccacttcct gcgcatgcag cagctggcca agacccagga ggagcgcgca cagctcctgc
1981agcccacgct ggagatcaac cccaggcacg cgctcatcaa gaagctgaat cagctgcgcg
2041caagcgagcc tggcctggct cagctgctgg tggatcagat atacgagaac gccatgattg
2101ctgctggact tgttgacgac cctagggcca tggtgggccg cttgaatgag ctgcttgtca
2161aggccctgga gcgacactgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
2221accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2281agtcgacaat caacctctgg atta

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