Construct: ORF ccsbBroad304_02362
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005620.1_s304c1, BRDN0000399717
- Derived from:
- ccsbBroadEn_02362
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX6 (10228)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02362
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10228 | STX6 | syntaxin 6 | NM_005819.6 | 100% | 100% | |
| 2 | human | 10228 | STX6 | syntaxin 6 | NM_001286210.1 | 60.3% | 60.3% | 0_1ins303 |
| 3 | human | 10228 | STX6 | syntaxin 6 | XM_017000007.1 | 60.3% | 60.3% | 0_1ins303 |
| 4 | mouse | 58244 | Stx6 | syntaxin 6 | NM_021433.3 | 90.8% | 94.5% | (many diffs) |
| 5 | mouse | 58244 | Stx6 | syntaxin 6 | XM_006529766.3 | 83.8% | 82.8% | (many diffs) |
| 6 | mouse | 58244 | Stx6 | syntaxin 6 | XM_017321868.1 | 53.7% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 831
- ORF length:
- 765
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc catggaggac cccttctttg tggtgaaagg agaggtacag aaagcagtca 121 acactgccca gggattgttt cagagatgga cagagctcct ccaggacccc tccacagcaa 181 caagggaaga aatcgactgg accaccaacg agctgagaaa taacctccgg agcatagagt 241 gggatctaga ggaccttgat gaaaccatca gcatagttga agcaaatcct agaaaattta 301 accttgatgc aactgaattg agtataagaa aagccttcat tacaagtact cggcaagttg 361 tcagggacat gaaagatcag atgtcaactt catctgtgca ggcattagct gaaagaaaaa 421 atagacaggc actgctggga gacagtggca gccagaactg gagcactgga acaacagata 481 aatatgggcg tctggaccga gagctccaga gagccaattc tcatttcatt gaggagcagc 541 aggcacagca gcagttgatc gtggaacagc aggatgagca gttggagctg gtctctggca 601 gcatcggggt gctgaagaac atgtcccagc gcatcggagg ggagctggag gaacaggcag 661 ttatgttgga agatttctct cacgaattgg agagcactca gtcccggctg gacaatgtga 721 tgaagaaact tgcaaaagta tctcatatga ccagtgatcg gcgccaatgg tgtgccatag 781 ccatcctctt tgcagtcctg ttggttgtgc tcatcctctt cttagtgctg tgcccaactt 841 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 901 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta