Construct: ORF ccsbBroad304_02516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004023.1_s304c1, BRDN0000403764
- Derived from:
- ccsbBroadEn_02516
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAG2 (10735)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02516
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10735 | STAG2 | stromal antigen 2 | NM_001042751.2 | 100% | 100% | |
| 2 | human | 10735 | STAG2 | stromal antigen 2 | NM_001282418.2 | 100% | 100% | |
| 3 | human | 10735 | STAG2 | stromal antigen 2 | NM_006603.5 | 100% | 100% | |
| 4 | human | 10735 | STAG2 | stromal antigen 2 | XM_005262361.2 | 100% | 100% | |
| 5 | human | 10735 | STAG2 | stromal antigen 2 | XM_017029232.1 | 100% | 100% | |
| 6 | human | 10735 | STAG2 | stromal antigen 2 | XM_017029233.1 | 100% | 100% | |
| 7 | human | 10735 | STAG2 | stromal antigen 2 | XM_017029234.1 | 100% | 100% | |
| 8 | human | 10735 | STAG2 | stromal antigen 2 | XM_024452328.1 | 100% | 100% | |
| 9 | human | 10735 | STAG2 | stromal antigen 2 | XM_024452329.1 | 100% | 100% | |
| 10 | human | 10735 | STAG2 | stromal antigen 2 | NM_001042749.2 | 97% | 97% | 3468_3578del |
| 11 | human | 10735 | STAG2 | stromal antigen 2 | NM_001042750.2 | 97% | 97% | 3468_3578del |
| 12 | human | 10735 | STAG2 | stromal antigen 2 | XM_005262357.3 | 97% | 97% | 3468_3578del |
| 13 | human | 10735 | STAG2 | stromal antigen 2 | XM_005262358.2 | 97% | 97% | 3468_3578del |
| 14 | human | 10735 | STAG2 | stromal antigen 2 | XM_005262359.4 | 97% | 97% | 3468_3578del |
| 15 | human | 10735 | STAG2 | stromal antigen 2 | XM_005262360.2 | 97% | 97% | 3468_3578del |
| 16 | human | 10735 | STAG2 | stromal antigen 2 | XM_006724727.1 | 97% | 97% | 3468_3578del |
| 17 | human | 10735 | STAG2 | stromal antigen 2 | XM_011531253.2 | 97% | 97% | 3468_3578del |
| 18 | human | 10735 | STAG2 | stromal antigen 2 | XM_024452326.1 | 97% | 97% | 3468_3578del |
| 19 | human | 10735 | STAG2 | stromal antigen 2 | XM_024452327.1 | 97% | 97% | 3468_3578del |
| 20 | human | 10735 | STAG2 | stromal antigen 2 | XM_006724728.4 | 91.6% | 91.6% | 0_1ins207;3261_3371del |
| 21 | human | 10735 | STAG2 | stromal antigen 2 | XM_017029235.1 | 91.6% | 91.6% | 0_1ins207;3261_3371del |
| 22 | mouse | 20843 | Stag2 | stromal antigen 2 | NM_001077712.2 | 93.9% | 99.1% | (many diffs) |
| 23 | mouse | 20843 | Stag2 | stromal antigen 2 | NM_021465.4 | 93.9% | 99.1% | (many diffs) |
| 24 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250989.2 | 93.9% | 99.1% | (many diffs) |
| 25 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_017318446.1 | 93.9% | 99.1% | (many diffs) |
| 26 | mouse | 20843 | Stag2 | stromal antigen 2 | NM_001290713.1 | 91.1% | 96.2% | (many diffs) |
| 27 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250983.2 | 91.1% | 96.2% | (many diffs) |
| 28 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250984.2 | 91.1% | 96.2% | (many diffs) |
| 29 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250985.2 | 91.1% | 96.2% | (many diffs) |
| 30 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250986.2 | 91.1% | 96.2% | (many diffs) |
| 31 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250987.1 | 91.1% | 96.2% | (many diffs) |
| 32 | mouse | 20843 | Stag2 | stromal antigen 2 | XM_011250988.1 | 91.1% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3759
- ORF length:
- 3693
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat agcagctcca gaaataccaa ctgattttaa tctactacag gagtcagaaa 121 cacatttttc ttctgacaca gattttgaag atatcgaagg aaaaaaccaa aagcaaggca 181 aaggcaaaac ttgtaaaaaa ggcaaaaagg gcccagcaga aaagggcaaa ggtggaaatg 241 gaggaggaaa acctccttct ggtccaaacc gaatgaatgg tcatcaccaa cagaatggag 301 tggaaaacat gatgttgttt gaagttgtta aaatgggcaa gagtgctatg cagtcggtgg 361 tagatgattg gatagaatca tacaagcatg accgagatat agcacttctt gaccttatca 421 acttttttat tcagtgttca ggctgtaaag gagttgtcac agcagaaatg tttagacata 481 tgcagaactc tgagataatt cgaaaaatga ctgaagaatt cgatgaggat agtggagatt 541 atccacttac catggctggt cctcagtgga agaagttcaa atccagtttt tgtgaattca 601 ttggcgtgtt agtacggcaa tgtcaatata gtatcatata tgatgagtat atgatggata 661 cagtcatttc acttcttaca ggattgtctg actcacaagt cagagcattt cgacatacaa 721 gcaccctggc agctatgaag ttgatgacag ctttggtgaa tgtggcacta aatcttagca 781 ttaatatgga taatacacaa agacaatatg aagcagaacg gaataaaatg attggaaaac 841 gagccaatga gaggctagaa ctcctgctac aaaagcggaa agagcttcag gaaaatcaag 901 atgaaataga aaatatgatg aatgcaatat ttaaaggagt gtttgtacat agataccgtg 961 atgcgatagc tgaaattcga gctatttgca ttgaagagat tggcatttgg atgaagatgt 1021 atagtgatgc ctttcttaat gacagttatt taaaatatgt tggttggact atgcatgata 1081 agcaaggtga agtaagactc aaatgtctta ctgctctaca agggctttat tataacaaag 1141 agcttaattc caaactggaa ctttttacca gtcggttcaa ggatagaatt gtgtctatga 1201 cccttgacaa agaatatgat gttgcagtac aagcaataaa attactcact cttgttttac 1261 agagtagtga agaagttctc actgcagaag attgtgaaaa tgtctatcat ctggtttatt 1321 cagctcaccg gccagtagca gtagcagctg gagaatttct ctacaaaaag ctcttcagtc 1381 gtagagatcc agaggaggat ggaatgatga aaagaagagg aagacaaggt ccaaatgcca 1441 accttgttaa gacattggtt tttttctttc tagaaagtga gttacatgag catgcagcat 1501 accttgtgga tagcatgtgg gactgtgcta ctgagctgct gaaagactgg gaatgtatga 1561 atagcttgtt actggaagag ccacttagtg gagaggaagc actaacagat aggcaagaga 1621 gtgctctgat tgaaataatg ctttgtacca ttagacaagc ggctgaatgt catcctcccg 1681 tgggaagagg gacaggaaaa agggtgctta cagcaaagga gaagaagaca cagttggatg 1741 ataggacaaa aatcactgag ctttttgccg tggcccttcc tcagttatta gcaaaatact 1801 ctgtagatgc agaaaaggtg actaacttgt tgcagttgcc tcagtacttt gatttggaaa 1861 tatataccac tggacgatta gaaaagcatt tggatgcctt attgcgacag atccggaata 1921 ttgtagagaa gcacacagat acagatgttt tggaagcatg ttctaaaact taccatgcac 1981 tctgtaatga agagttcaca atcttcaaca gagtagatat ttcaagaagt caactgatag 2041 atgaattggc agataaattt aaccggcttc ttgaagattt tctgcaagag ggtgaagaac 2101 ctgatgaaga tgatgcatat caggtattgt caacattgaa gaggatcact gcttttcata 2161 atgcccatga cctttcaaag tgggatttat ttgcttgtaa ttacaaactc ttgaaaactg 2221 gaatcgaaaa tggagacatg cctgagcaga ttgttattca cgcactgcag tgtactcact 2281 atgtaatcct ttggcaactt gctaagataa ctgaaagcag ctctacaaag gaggacttgc 2341 tgcgtttaaa gaaacaaatg agagtatttt gtcagatatg tcaacattac ctgaccaacg 2401 tgaatactac tgttaaggaa caggccttca ctattctgtg tgatattttg atgatcttca 2461 gccatcagat tatgtcagga gggcgtgaca tgttagagcc attagtgtat acccctgatt 2521 cttcattgca gtctgagttg ctcagcttta ttttggatca tgtcttcatt gaacaggatg 2581 atgataataa tagtgcagat ggtcagcaag aggatgaagc cagtaaaatt gaagctctgc 2641 acaagagaag aaatttactt gcagcatttt gtaagctaat tgtatatact gtggtggaga 2701 tgaatacagc tgcagatatc ttcaaacagt atatgaagta ttataatgac tatggagata 2761 tcatcaaaga aacaatgagt aaaacaaggc agatagacaa aattcagtgt gctaagaccc 2821 ttattctcag tctgcaacag ctttttaatg aaatgataca agaaaatggc tataattttg 2881 atagatcatc ctctacattt agtggcataa aagaacttgc tcgacgtttt gctttaactt 2941 ttggacttga tcagttgaaa acaagagaag ccattgccat gctacacaaa gatggcatag 3001 aatttgcttt taaagagcct aatccgcaag gggagagcca tccaccttta aatttggcat 3061 ttcttgatat tctgagtgaa ttttcttcta aactacttcg acaagacaaa agaacagtgt 3121 atgtttactt ggaaaagttc atgacctttc agatgtcact ccgaagagag gatgtgtggc 3181 ttccactgat gtcttaccga aattctttgc tagctggtgg tgatgatgac accatgtcag 3241 tcattagtgg aatcagcagc cgggggtcaa cagtacggag taaaaaatca aaaccatcta 3301 caggaaaacg gaaagtggtt gagggcatgc agctttcact cactgaagaa agtagtagta 3361 gtgacagtat gtggttaagc agagaacaaa cactgcacac ccctgttatg atgcagacac 3421 cacaactcac ctccactatt atgagagagc ccaaaagatt acggcctgag gatagcttca 3481 tgagtgttta tccaatgcag actgaacatc atcaaacacc tcttgattat aatcggcgtg 3541 gcacaagcct aatggaagat gatgaagagc caattgtgga agatgttatg atgtcctcag 3601 aagggaggat tgaggatctt aatgagggaa tggattttga caccatggat atagatttgc 3661 caccatcaaa gaacagacga gagagaacag aactgaagcc tgatttcttt gatccagctt 3721 caattatgga tgaatcagtt cttggagtgt caatgtttta cccaactttc ttgtacaaag 3781 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 3841 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta