Construct: ORF ccsbBroad304_02581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007869.1_s304c1, BRDN0000407735
- Derived from:
- ccsbBroadEn_02581
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASCC3 (10973)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02581
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10973 | ASCC3 | activating signal cointegra... | NM_022091.4 | 100% | 100% | |
| 2 | human | 10973 | ASCC3 | activating signal cointegra... | NM_001284271.1 | 13.9% | 11.7% | (many diffs) |
| 3 | human | 10973 | ASCC3 | activating signal cointegra... | NM_006828.4 | 4.6% | 3.9% | (many diffs) |
| 4 | human | 10973 | ASCC3 | activating signal cointegra... | XM_011535394.3 | 4.6% | 3.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 399
- ORF length:
- 333
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tttacctcgt ctcacaggag ccttgcgttc cttttcaaat gtcaccaagc 121 aagataatta taatgaagaa gtggctgact taaagataaa gcgatctaaa cttcatgaac 181 aagttttaga tttgggcctg acatggaaga agataataaa atttttgaat gaaaaactgg 241 agaagagtaa aatgcaaagt ataaatgaag acttaaaaga tatattacat gctgcaaagc 301 agatagaagt gaattgtcca ttccagaaga ggaggctgga tggaaaagag gaggatgaga 361 agatgagcag agcttctgac agattcagag gactaagatg cccaactttc ttgtacaaag 421 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 481 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta