Construct: ORF ccsbBroad304_02704

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004433.1_s304c1, BRDN0000399958
Derived from:
ccsbBroadEn_02704
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEPHS1 (22929)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02704
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22929 SEPHS1 selenophosphate synthetase 1 NM_012247.5 100% 100%
2 human 22929 SEPHS1 selenophosphate synthetase 1 XM_017015943.2 100% 100%
3 human 22929 SEPHS1 selenophosphate synthetase 1 XM_017015944.2 99.4% 99.4% 748_749insCAGCTG
4 human 22929 SEPHS1 selenophosphate synthetase 1 NM_001195602.1 82.9% 82.9% 0_1ins201
5 human 22929 SEPHS1 selenophosphate synthetase 1 XM_017015945.2 82.9% 82.9% 0_1ins201
6 human 22929 SEPHS1 selenophosphate synthetase 1 NM_001195604.2 81.8% 81.8% 748_749ins213
7 mouse 109079 Sephs1 selenophosphate synthetase 1 NM_175400.6 89.7% 99.4% (many diffs)
8 mouse 109079 Sephs1 selenophosphate synthetase 1 XM_006497309.2 89.7% 99.4% (many diffs)
9 mouse 109079 Sephs1 selenophosphate synthetase 1 XM_017314904.1 89.7% 99.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1242
ORF length:
1176
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tacgcgggag tcctttaacc cggaaagtta cgaattggac aaaagcttcc
121ggctaaccag attcactgaa ctgaagggca caggctgcaa agtgccccaa gatgtcctgc
181aaaaattgct ggaatcttta caggagaacc acttccaaga agatgagcag tttctgggag
241ccgttatgcc aaggcttggc attggaatgg atacttgtgt cattcctttg aggcacggtg
301ggctttcctt ggttcaaacc acagattaca tttacccgat cgtagacgac ccttacatga
361tgggcaggat agcgtgtgcc aatgtcctca gtgacctcta tgcaatgggg gtcacggaat
421gtgacaatat gctgatgctc cttggagtca gtaataaaat gaccgacagg gaaagggata
481aagtgatgcc tctgattatc caaggtttta aagacgcagc tgaggaagca ggaacatctg
541taacaggcgg ccaaacagta ctaaacccct ggattgtcct gggaggagtg gctaccactg
601tctgccaacc caatgaattt atcatgccag acaatgcagt gccaggggac gtgctggtgc
661tgacaaaacc cctggggaca caggtggcag tggctgtgca ccagtggctg gatatccctg
721agaaatggaa taagattaaa ctagtggtca cccaagaaga tgtagagctg gcctaccagg
781aggcgatgat gaacatggcg aggctcaaca ggacagctgc aggactcatg cacacgttca
841atgcccacgc cgccactgac atcacgggct tcgggatttt gggccatgcg cagaacctgg
901ccaagcagca gaggaacgag gtgtcgtttg taattcacaa cctcccggtg ctggccaaga
961tggctgcggt gagcaaggcc tgcggaaaca tgttcggcct catgcacggg acctgcccgg
1021agacttcagg cggccttctg atctgtttac cacgtgagca agcagctcgg ttctgtgcag
1081agataaagtc ccccaaatat ggtgaaggcc accaagcatg gattattggg attgtagaga
1141agggcaaccg cacagccaga atcatagaca aaccccggat catcgaggtc gcaccacaag
1201tggccactca aaatgtgaat cccacacccg gggccacctc ttacccaact ttcttgtaca
1261aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1321ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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