Construct: ORF ccsbBroad304_02743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003504.1_s304c1, BRDN0000403722
- Derived from:
- ccsbBroadEn_02743
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIP5K2 (23262)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02743
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_015216.5 | 99.9% | 100% | 3124A>C |
| 2 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_005277536.2 | 98.7% | 98.7% | 2530_2574del;3169A>C |
| 3 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001276277.3 | 98.2% | 98.3% | 3124A>C;3287_3349del |
| 4 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345873.2 | 98.2% | 98.2% | 1613_1614insAGG;3121A>C;3284_3346del |
| 5 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_005277534.2 | 97.1% | 97.1% | 2530_2574del;3169A>C;3332_3394del |
| 6 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_005277535.2 | 97% | 97% | (many diffs) |
| 7 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345876.2 | 96.6% | 96.7% | 3124A>C;3168_3169ins120 |
| 8 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345878.2 | 96.6% | 96.6% | 1613_1614insAGG;3121A>C;3165_3166ins120 |
| 9 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_006714576.2 | 96.6% | 95.8% | (many diffs) |
| 10 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001281471.3 | 95.5% | 95.6% | 2530_2574del;3169A>C;3333_3455del |
| 11 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_005277539.2 | 95.5% | 95.5% | 2530_2574del;3169A>C;3213_3214ins120 |
| 12 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345874.2 | 95.2% | 95.2% | 2922_2923ins174;2950A>C |
| 13 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_017009273.2 | 95.1% | 95.1% | 1613_1614insAGG;2919_2920ins174;2947A>C |
| 14 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345871.2 | 93.6% | 93.6% | 2922_2923ins174;2950A>C;3113_3175del |
| 15 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_017009274.2 | 93% | 92.3% | (many diffs) |
| 16 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345872.2 | 92.5% | 92.5% | (many diffs) |
| 17 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345875.2 | 91.9% | 91.9% | 2922_2923ins174;2950A>C;2994_2995ins120 |
| 18 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_006714577.2 | 91.9% | 91.2% | (many diffs) |
| 19 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | NM_001345877.2 | 91.8% | 91.8% | (many diffs) |
| 20 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_005277541.2 | 90.8% | 90.8% | (many diffs) |
| 21 | human | 23262 | PPIP5K2 | diphosphoinositol pentakisp... | XM_011543290.3 | 80.7% | 79.8% | (many diffs) |
| 22 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529493.2 | 89% | 94.8% | (many diffs) |
| 23 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_017320371.1 | 89% | 94.8% | (many diffs) |
| 24 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529489.1 | 87.5% | 93.2% | (many diffs) |
| 25 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529490.3 | 87.5% | 93.2% | (many diffs) |
| 26 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529492.2 | 87.5% | 93.2% | (many diffs) |
| 27 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529491.2 | 87.4% | 93.1% | (many diffs) |
| 28 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529494.2 | 87% | 91.9% | (many diffs) |
| 29 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529496.2 | 85.9% | 91.6% | (many diffs) |
| 30 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_017320374.1 | 85.9% | 91.6% | (many diffs) |
| 31 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_011247990.1 | 84.3% | 88.8% | (many diffs) |
| 32 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529495.2 | 83% | 88.7% | (many diffs) |
| 33 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529497.2 | 82.4% | 87.3% | (many diffs) |
| 34 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | NM_173760.5 | 81.3% | 86.9% | (many diffs) |
| 35 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529499.3 | 81.3% | 86.8% | (many diffs) |
| 36 | mouse | 227399 | Ppip5k2 | diphosphoinositol pentakisp... | XM_006529500.2 | 70.7% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3732
- ORF length:
- 3666
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tgaagccccc agattcttcg ttggaccaga agatacagaa ataaatcctg 121 gaaattatcg acatttcttc caccatgcag atgaagacga tgaggaggaa gatgattctc 181 caccagaaag gcagattgtg gttggaatat gttccatggc aaagaaatcc aaatccaaac 241 caatgaagga aattcttgaa cggatctcct tatttaaata tatcacagta gtagtatttg 301 aagaggaggt tattttgaat gaaccagtgg aaaactggcc tttatgtgat tgtcttattt 361 ctttccattc taaaggattt ccactggaca aagcggttgc ctatgcaaaa ctcaggaatc 421 catttgtaat caatgacttg aatatgcagt atctcataca agataggaga gaagtatata 481 gtattcttca agctgaaggt attttacttc ctcgttatgc tattttgaac cgtgacccaa 541 ataatcccaa agaatgtaat ctgattgaag gggaagatca tgtagaagta aatggggaag 601 tttttcaaaa gccatttgta gaaaagccag tcagtgcaga agatcacaat gtttacattt 661 attacccaac ttctgctggt ggtggaagtc aaagactctt tagaaagatt ggcagtagaa 721 gtagtgttta ttctccagaa agcaatgtac gaaaaacagg ctcatatata tatgaagagt 781 ttatgcccac agatggtact gatgttaagg tttatacagt gggtccagat tatgcccatg 841 ctgaagctcg aaaatctcca gcacttgatg gcaaggtgga acgagacagt gaaggaaaag 901 aagtaagata ccctgttatt ctcaatgcac gagagaaatt aattgcttgg aaagtctgcc 961 ttgcttttaa gcaaacagtt tgtggctttg atttgttacg ggccaatgga cagtcctatg 1021 tctgtgatgt caatggcttc agttttgtga aaaattccat gaagtattat gatgactgtg 1081 caaaaatact tggaaatatt gtaatgcgag aacttgctcc acaatttcat attccatggt 1141 caataccctt agaagctgaa gatatcccaa ttgtaccaac tacatctgga actatgatgg 1201 aacttagatg tgtcatagct gttatacgtc atggggatcg aacaccaaaa caaaaaatga 1261 aaatggaagt gagacatcag aaattttttg atctttttga aaagtgtgat ggatataaat 1321 cagggaaatt aaaactcaaa aaaccaaaac agttacagga agtgctagat attgcacgac 1381 agcttcttat ggagctaggg caaaataatg attctgaaat tgaagaaaac aagccaaaac 1441 ttgaacaact taagactgta ttagagatgt atggtcattt ttctggaata aatcgtaagg 1501 ttcagttgac ctatctccct catggttgtc ctaaaacatc tagtgaagag gaggacagcc 1561 gaagagaaga accatcttta cttttggttc taaaatgggg aggtgaatta actcctgcag 1621 gcagggtcca ggctgaagaa cttggaagag ccttcaggtg tatgtatcct ggaggtcaag 1681 gagattatgc aggatttcct ggttgtggtt tacttagatt acatagcacc tacagacatg 1741 acctcaaaat atatgcctct gatgaaggac gagtccagat gactgcagct gcttttgcaa 1801 aggggctttt agctttggaa ggagagctta cacccattct tgttcaaatg gtgaaaagtg 1861 caaatatgaa cggtcttttg gatagtgata gtgactctct gagcagttgt cagcaacgtg 1921 tgaaggcaag gcttcatgaa atacttcaga aagacagaga ttttactgct gaagattatg 1981 aaaagcttac tccatctgga agcatttctc ttatcaaatc aatgcattta attaaaaacc 2041 ctgtgaagac ctgtgataaa gtttattcct taattcagag tttgacttct caaatcagac 2101 atcgaatgga agatcctaaa tcatcagata ttcagcttta ccatagtgaa acattggagc 2161 ttatgctacg tagatggtcc aagttagaga aagactttaa aacaaagaat ggaagatatg 2221 atattagtaa aatccctgac atatatgact gtataaaata tgatgtccag cataatggtt 2281 ccttgaaatt agaaaacaca atggaattat ataggctttc gaaggcatta gcagatattg 2341 ttatccctca ggaatatggt ataactaaag ctgaaaaact ggagattgcc aaaggctact 2401 gtactcctct ggttagaaaa attcgctcag accttcagag gacacaagat gatgacactg 2461 taaataaact tcatcctgtg tattctagag gtgttctgtc tcctgaacgt catgttcgta 2521 ctagattata ttttaccagt gaaagtcatg tacattcttt gctgtctatt cttcgctatg 2581 gtgccttatg caatgaatca aaggatgaac agtggaaacg agctatggat tatttaaacg 2641 ttgtcaatga gctcaactac atgactcaga ttgttatcat gctttatgag gatcctaata 2701 aggatctttc ctcagaagaa cgctttcatg ttgaattaca ctttagtccg ggagccaaag 2761 gttgtgaaga agacaaaaat ttgccatctg gctatggata tagaccagct tccagagaga 2821 atgaaggcag gagacctttt aaaattgata atgatgatga accacatact tctaaaagag 2881 atgaagttga tcgagctgtg atattgttta aaccaatggt atcagagcca attcatatac 2941 acaggaagtc tccacttcca agatctagga agacggctac aaatgatgaa gagagccccc 3001 tgagtgtgtc tagcccagag ggtactggta cctggctgca ttataccagt ggtgtgggta 3061 ctgggcgtcg aagacgcaga tcaggggaac aaatcacttc ttcccctgtc tcccccaaat 3121 cattggcttt cacatccagt atttttggct catggcaaca ggttgtatct gaaaatgcta 3181 attacctgcg aacaccaaga actcttgtgg aacagaagca gaatcctact gtagggtctc 3241 actgtgcggg cctgtttagc acctcggtgc tcgggggttc ttcaagcgca cctaacctac 3301 aggattatgc tcgtactcat cgtaaaaagc tgacctcttc tggctgcata gatggctttg 3361 aattgtattc catggtgcca tctatttgtc ctctagaaac tcttcataat gccctatctt 3421 taaagcaagt ggatgaattt cttgcttcca ttgcttctcc atcatctgac gttccacgga 3481 agaccgctga aatttcctct acagctttac gttccagtcc aataatgaga aaaaaagtat 3541 ctttaaatac gtatacacct gcaaagatcc tcccaacacc accagctacc ttaaagagca 3601 ctaaagcaag cagcaaacca gctacaagtg gaccttctag tgcagttgtt cctaatacct 3661 catctcggaa aaagaatata actagcaaaa cagaaacgca tgaacacaaa aaaaacactg 3721 ggaaaaagaa atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 3781 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 3841 caatcaacct ctggatta