Construct: ORF ccsbBroad304_02747

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015499.1_s304c1, BRDN0000400827
Derived from:
ccsbBroadEn_02747
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MGRN1 (23295)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02747
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23295 MGRN1 mahogunin ring finger 1 NM_015246.4 100% 100%
2 human 23295 MGRN1 mahogunin ring finger 1 NM_001142289.2 96.1% 96.1% 1065_1066ins66
3 human 23295 MGRN1 mahogunin ring finger 1 NM_001142290.2 95.4% 93.9% (many diffs)
4 human 23295 MGRN1 mahogunin ring finger 1 NM_001142291.2 91.6% 90.1% (many diffs)
5 human 23295 MGRN1 mahogunin ring finger 1 NR_102267.1 39.4% 1_137del;1092_1093ins176;1690_3758del
6 mouse 17237 Mgrn1 mahogunin, ring finger 1 XM_006521832.1 85.5% 88.6% (many diffs)
7 mouse 17237 Mgrn1 mahogunin, ring finger 1 XM_017316887.1 82% 85% (many diffs)
8 mouse 17237 Mgrn1 mahogunin, ring finger 1 XM_006521834.1 81.9% 84.9% (many diffs)
9 mouse 17237 Mgrn1 mahogunin, ring finger 1 NM_029657.4 78.4% 81.3% (many diffs)
10 mouse 17237 Mgrn1 mahogunin, ring finger 1 NM_001252437.1 78.3% 81.2% (many diffs)
11 mouse 17237 Mgrn1 mahogunin, ring finger 1 XM_006521837.1 63.6% 62.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1794
ORF length:
1728
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg ctccattctc agccgccgca tcgcgggggt ggaggacatc gacatccagg
121cgaactcggc ctatcgctac cctccgaagt ccggaaacta ctttgcttcg cactttttca
181tgggaggaga gaaattcgac accccccacc ctgaaggtta cctctttgga gagaacatgg
241atctgaactt cctgggcagc cgcccggtcc agtttcccta cgtcactcct gccccccacg
301agcccgtgaa gacgctgcgg agcctggtga acatccgcaa agactccctg cggctggtga
361ggtacaaaga cgatgccgac agccccaccg aggacggcga caagccccgg gtgctctaca
421gcctggagtt caccttcgac gccgatgccc gcgtggccat caccatctac tgccaggcat
481cggaggagtt cctgaacggc agggcagtat acagccccaa gagcccctcg ctacagtccg
541agaccgtcca ctacaagaga ggggtgagcc agcagttctc cctgccctcc ttcaagattg
601acttctcgga atggaaggat gacgagctga actttgacct ggaccggggc gtgtttccag
661tagtcatcca ggctgtggtg gacgaaggag atgtggtgga agtgactggc cacgcccacg
721tgctcttggc tgcctttgaa aagcacatgg acggcagctt ctctgtgaag cctttaaagc
781agaagcaaat tgtggaccgg gtcagctacc tcctgcagga gatctatggc attgagaaca
841agaacaacca ggagaccaag ccctcggacg acgagaacag cgacaacagc aacgagtgtg
901tggtgtgcct gtccgacctg cgggacacgc tgatcctgcc ctgccgccac ctgtgcctct
961gtacctcctg cgccgacacg ctgcgctacc aggccaacaa ctgccccatc tgccggctgc
1021ctttccgggc cctcctgcag atccgggcgg tgcggaagaa gccaggagcc ctgtcccccg
1081tgtccttcag ccccgtcctg gcccagagcc tggagcatga tgagcactct tgtcccttta
1141aaaaatcaaa gccgcacccc gcctccctgg ccagcaagaa acctaaaagg gaaacaaact
1201ctgacagcgt cccacctggc tacgagccca tctcgctgct cgaggcgctc aacggcctcc
1261gggctgtctc cccggccatc ccctcggccc ctctttatga agaaatcacc tattcaggca
1321tctcggacgg cctgtcccag gccagctgtc ccctcgcggc tatcgaccac atcctggaca
1381gcagccgcca gaagggcagg ccgcagagca aggcccccga cagcacccta cggtccccgt
1441cttcccccat ccacgaagag gatgaggaga agctctccga ggacgtggac gcccctcccc
1501cactgggtgg cgcagagctg gccctgcggg aaagcagctc ccctgagagt ttcataacag
1561aagaggttga tgagtcgtcg tcaccacagc aagggacccg agcagcttcc attgagaatg
1621tcctgcagga cagcagcccc gagcactgtg gccgaggccc acctgctgac atctacctgc
1681caggacggcc cacctccatg gagacggccc acggcctcgc caccaccagc cccacctggc
1741ctccacttgg tggccccagc cccgatccca gcgccgccga gctgacccca ctctgcccaa
1801ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1861cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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