Transcript: Human NM_001142291.2

Homo sapiens mahogunin ring finger 1 (MGRN1), transcript variant 4, mRNA.

Source:
NCBI, updated 2018-09-22
Taxon:
Homo sapiens (human)
Gene:
MGRN1 (23295)
Length:
6405
CDS:
138..1730

Additional Resources:

NCBI RefSeq record:
NM_001142291.2
NBCI Gene record:
MGRN1 (23295)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001142291.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033792 CGGAAACTACTTTGCTTCGCA pLKO.1 224 CDS 100% 0.750 1.050 N MGRN1 n/a
2 TRCN0000333019 CGGAAACTACTTTGCTTCGCA pLKO_005 224 CDS 100% 0.750 1.050 N MGRN1 n/a
3 TRCN0000033790 CCTGAGAGTTTCATAACAGAA pLKO.1 1548 CDS 100% 4.950 3.465 N MGRN1 n/a
4 TRCN0000333084 CCTGAGAGTTTCATAACAGAA pLKO_005 1548 CDS 100% 4.950 3.465 N MGRN1 n/a
5 TRCN0000033793 GATTGACTTCTCGGAATGGAA pLKO.1 668 CDS 100% 3.000 2.100 N MGRN1 n/a
6 TRCN0000333020 GATTGACTTCTCGGAATGGAA pLKO_005 668 CDS 100% 3.000 2.100 N MGRN1 n/a
7 TRCN0000033789 CCCTTGCAGTTCTCTCTGATT pLKO.1 5495 3UTR 100% 4.950 2.475 Y MGRN1 n/a
8 TRCN0000333022 CCCTTGCAGTTCTCTCTGATT pLKO_005 5495 3UTR 100% 4.950 2.475 Y MGRN1 n/a
9 TRCN0000039466 GAAACTACTTTGCTTCGCATT pLKO.1 226 CDS 100% 4.050 5.670 N Mgrn1 n/a
10 TRCN0000033791 TGGAGCATGATGAGCACTCTT pLKO.1 1183 CDS 100% 4.950 3.465 N MGRN1 n/a
11 TRCN0000333021 TGGAGCATGATGAGCACTCTT pLKO_005 1183 CDS 100% 4.950 3.465 N MGRN1 n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3011 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001142291.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02747 pDONR223 100% 91.6% 90.1% None (many diffs) n/a
2 ccsbBroad304_02747 pLX_304 0% 91.6% 90.1% V5 (many diffs) n/a
3 TRCN0000466993 CTGAGGTAACTTTCACCTCCACCT pLX_317 20.8% 91.6% 90.1% V5 (many diffs) n/a
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