Construct: ORF ccsbBroad304_02809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001067.1_s304c1, BRDN0000411955
- Derived from:
- ccsbBroadEn_02809
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAPK2 (23604)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02809
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23604 | DAPK2 | death associated protein ki... | NM_014326.5 | 100% | 100% | |
| 2 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022044.1 | 93.8% | 94% | (many diffs) |
| 3 | human | 23604 | DAPK2 | death associated protein ki... | XM_011521414.1 | 91.2% | 90.6% | (many diffs) |
| 4 | human | 23604 | DAPK2 | death associated protein ki... | XM_011521413.2 | 91.2% | 88.8% | (many diffs) |
| 5 | human | 23604 | DAPK2 | death associated protein ki... | XM_011521417.1 | 85.4% | 74.4% | 810_811ins46;948_949ins116 |
| 6 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022045.1 | 85.4% | 74.4% | 810_811ins46;948_949ins116 |
| 7 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022046.1 | 85.4% | 74.4% | 810_811ins46;948_949ins116 |
| 8 | human | 23604 | DAPK2 | death associated protein ki... | XM_011521415.3 | 80.8% | 70.9% | (many diffs) |
| 9 | human | 23604 | DAPK2 | death associated protein ki... | NM_001363730.2 | 71.2% | 59.8% | (many diffs) |
| 10 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022043.1 | 55.6% | 44.6% | (many diffs) |
| 11 | human | 23604 | DAPK2 | death associated protein ki... | XM_011521421.3 | 51.9% | 49.4% | (many diffs) |
| 12 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022047.1 | 39.7% | 29.3% | (many diffs) |
| 13 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022048.2 | 39.7% | 29.3% | (many diffs) |
| 14 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022049.1 | 39.7% | 29.3% | (many diffs) |
| 15 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022050.1 | 39.7% | 29.3% | (many diffs) |
| 16 | human | 23604 | DAPK2 | death associated protein ki... | XM_017022051.1 | 39.7% | 29.3% | (many diffs) |
| 17 | mouse | 13143 | Dapk2 | death-associated protein ki... | NM_010019.3 | 91.8% | 97.2% | (many diffs) |
| 18 | mouse | 13143 | Dapk2 | death-associated protein ki... | XM_006510811.2 | 52.7% | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1179
- ORF length:
- 1110
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttccaggcc tcaatgagga gtccaaacat ggagccattc aagcagcaga 121 aggtggagga cttttatgac atcggagagg agctggggag tggccagttt gccatcgtga 181 agaagtgccg ggagaagagc acggggcttg agtatgcagc caagttcatc aagaagcggc 241 agagccgggc gagccggcgc ggtgtgagcc gggaggagat cgagcgggag gtgagcatcc 301 tgcggcaggt gctgcaccac aatgtcatca cgctgcacga cgtctatgag aaccgcaccg 361 acgtggtgct catccttgag ctagtgtctg gaggagagct cttcgatttc ctggcccaga 421 aggagtcact gagtgaggag gaggccacca gcttcattaa gcagatcctg gatggggtga 481 actaccttca cacaaagaaa attgctcact ttgatctcaa gccagaaaac attatgttgt 541 tagacaagaa tattcccatt ccacacatca agctgattga ctttggtctg gctcacgaaa 601 tagaagatgg agttgaattt aagaatattt ttgggacgcc ggaatttgtt gctccagaaa 661 ttgtgaacta cgagcccctg ggtctggagg ctgacatgtg gagcataggc gtcatcacct 721 acatcctctt aagtggagca tcccctttcc tgggagacac gaagcaggaa acactggcaa 781 atatcacagc agtgagttac gactttgatg aggaattctt cagccagacg agcgagctgg 841 ccaaggactt tattcggaag cttctggtta aagagacccg gaaacggctc acaatccaag 901 aggctctcag acacccctgg atcacgccgg tggacaacca gcaagccatg gtgcgcaggg 961 agtctgtggt caatctggag aacttcagga agcagtatgt ccgcaggcgg tggaagcttt 1021 ccttcagcat cgtgtccctg tgcaaccacc tcacccgctc gctgatgaag aaggtgcacc 1081 tgaggccgga tgaggacctg aggaactgtg agagtgacac tgaggaggac atcgccagga 1141 ggaaagccct ccacccacgg aggaggagca gcacctcctt gccaactttc ttgtacaaag 1201 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1261 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta