Construct: ORF ccsbBroad304_02975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001347.1_s304c1, BRDN0000462295
- Derived from:
- ccsbBroadEn_02975
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RANBP6 (26953)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02975
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26953 | RANBP6 | RAN binding protein 6 | NM_012416.4 | 100% | 100% | |
| 2 | human | 26953 | RANBP6 | RAN binding protein 6 | NM_001243202.2 | 68.1% | 68.1% | 193_194ins1056 |
| 3 | human | 26953 | RANBP6 | RAN binding protein 6 | XM_017014624.1 | 62.6% | 62.3% | (many diffs) |
| 4 | human | 26953 | RANBP6 | RAN binding protein 6 | NM_001243203.2 | 5.4% | 4.1% | (many diffs) |
| 5 | mouse | 240614 | Ranbp6 | RAN binding protein 6 | NM_177721.4 | 91.1% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3384
- ORF length:
- 3315
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcaacc gcgtctgcag gggtgccggc gaccgtgtca gaaaagcaag 121 agttttacca gcttctgaag aacctgatca atccaagctg tatggtgcgg aggcaagcag 181 aggaaatcta tgaaaatatc ccaggtctgt gtaagactac cttcctctta gatgccgtca 241 gaaatagaag agcaggttat gaggtgagac aaatggctgc cgcactgcta cgacggcttt 301 tgtcctctgg gtttgaggag gtttatccaa atctgcctgc tgatgttcag agagatgtca 361 agattgaact gattctggct gttaagttag aaacacatgc tagcatgagg aaaaaacttt 421 gtgatatttt tgcagtgctg gccaggaatt tgatagatga ggatggcact aaccactggc 481 cggaaggtct gaagtttctt attgattcaa tctactccaa aaatgtggtt ctatgggaag 541 ttgcacttca cgttttctgg cactttcctg ggatttttgg gacccaagag cggcatgatt 601 tggatatcat caaacggttg ttggaccagt gtattcaaga tcaagaacat ccagcaatca 661 ggacattatc cgctagagct gcagctgcat ttgtacttgc taatgagaat aatattgctc 721 ttttcaaaga ctttgcagac ttgcttcctg gaatcttaca ggctgtgaat gactcatgct 781 accaggatga tgattcagtg ctagaatccc ttgttgagat tgcagatacc gtacctaagt 841 acttgggtcc ttatttagaa gatactctac agttgagttt gaagttatgt ggagactcta 901 ggcttagtaa tctgcagcgc cagctggccc tcgaagttat agtgaccttg tctgaaactg 961 ccactccgat gttgaaaaaa catacaaata ttattgcaca ggcagttcct catatattag 1021 caatgatggt tgatctacaa gatgatgagg actgggtaaa tgctgatgaa atggaagaag 1081 atgattttga cagcaatgca gttgctgcgg agagtgcact agacagactg gcttgtgggc 1141 ttggtggaaa agttgtttta ccaatgacca aggagcatat catgcagatg cttcagagcc 1201 ctgactggaa gtatcgacat gctggattaa tggccttatc tgccattgga gaaggatgcc 1261 atcaacaaat ggaatcaatt ctagatgaaa cagttaactc cgttttgctt tttcttcagg 1321 atcctcatcc aagggtgagg gctgcagcct gtactacact tggacagatg gctacagatt 1381 ttgcacctaa tttccaaaag aaatttcatg aaacagtgat tgcagctctg ttacgtacca 1441 tggaaaatca aggtaatcag cgtgtgcaat cacatgcagc ttctgctctt attattttta 1501 ttgaagactg ccctaaatca ttgctagttc tatatgtgga tagtatggtg aaaaatctac 1561 attccgtctt ggtgattaaa cttcaagagt tgattcggaa tggaactaag ttggctttgg 1621 aacaacttgt gacaaccatt gcatcagttg cagatacaat agaagaaaaa tttgtcccat 1681 attatgatat attcatgccc tcactaaagc acattgttga gcttgctgtt cagaaggaac 1741 tcaagcttct gagaggaaaa actatcgagt gcattagcca tattggtctt gctgttggga 1801 aggaaaaatt tatgcaagat gcatcaaatg tgatgcagct gttgttgaag acacaatcag 1861 acttaaataa tatggaagat gatgaccctc agacctctta catggtttca gcatgggcta 1921 gaatgtgtaa aattcttgga aaagattttc aacagtacct tccactggtt atcgagcctc 1981 ttattaagac tgcttcagct aaacctgatg ttgctctctt agacacacag gatgtggaaa 2041 atatgagtga cgatgatggc tggcaatttg taaatcttgg agaccagcag agttttggaa 2101 ttaaaacttc aggacttgaa gcaaaagcaa ctgcttgcca aatgttggtt tactatgcta 2161 aggagttaag ggaagggttt gtggaatata cagaacaagt tgtgaagctg atggttcctt 2221 tactgaaatt ttatttccat gacaatgttc gagtggcagc agcagagtcc atgccttttc 2281 tcctggaatg tgcaagaatt cgtggcccag agtatcttgc acagatgtgg caattcatat 2341 gtgacccctt aatcaaggct attggtactg aaccagatac agatgtgctc tcagaaataa 2401 tgaattcttt tgcaaagtcc attgaagtta tgggagatgg ttgccttaat gatgaacact 2461 tggaagaact gggaggaata ctgaaagcaa aacttgaagg gcactttaaa aaccaagaat 2521 tgagacaggt gaaaagacag gaagaaaact atgatcaaca ggttgagatg tctctgcaag 2581 atgaggatga atgtgatgtt tatattctga ccaaagtatc agatattttg cactcattat 2641 ttagtactta taaggaaaag attttaccat ggtttgaaca actacttcca ttaattgtaa 2701 atctaatttg ttcaagtagg ccatggccag acagacagtg gggattgtgc atatttgatg 2761 acatcataga gcactgcagt ccaacttcat ttaaatatgt agaatatttt cggtggccaa 2821 tgctactaaa tatgcgagat aacaaccctg aagtcaggca agctgctgct tatggcctgg 2881 gtgtcatggc acagtttggt ggagatgatt atcgttcttt atgttcagaa gctgttccac 2941 ttctggtaaa agttattaag tgtgcaaatt ccaaaaccaa aaaaaatgtc attgctacag 3001 agaactgtat ctcagcaata gggaagattt tgaagtttaa gcctaactgt gtaaatgtag 3061 atgaagttct tccacactgg ttatcatggc ttccactgca tgaagataaa gaggaagcta 3121 ttcagacttt gagttttctc tgtgacctaa ttgaaagtaa ccacccagtt gtaattggtc 3181 caaataattc caatcttccc aaaataatca gtataattgc agaaggaaaa attaatgaga 3241 ctattaacta tgaggatcct tgtgccaaac gcctagctaa tgtcgtgcgt caggtacaga 3301 cttctgaaga tttatggttg gaatgtgtat cacaacttga tgatgaacag caggaagcct 3361 tacaggagtt gctaaatttt gctttgccaa ctttcttgta caaagtggtt ggtaagccta 3421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3481 gcgttaagtc gacaatcaac ctctggatta