Construct: ORF ccsbBroad304_03129

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000045.1_s304c1, BRDN0000399108
Derived from:
ccsbBroadEn_03129
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ERO1A (30001)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03129
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 30001 ERO1A endoplasmic reticulum oxido... NM_014584.3 100% 100%
2 human 30001 ERO1A endoplasmic reticulum oxido... XM_011536684.2 99.1% 99.1% 673_674insAAGGGACAAGTG
3 human 30001 ERO1A endoplasmic reticulum oxido... XM_024449554.1 94.1% 94.1% 1210_1296del
4 mouse 50527 Ero1l ERO1-like (S. cerevisiae) NM_015774.3 86.6% 91.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1470
ORF length:
1404
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg ccgcggctgg ggattcttgt ttggcctcct gggcgccgtg tggctgctca
121gctcgggcca cggagaggag cagcccccgg agacagcggc acagaggtgc ttctgccagg
181ttagtggtta cttggatgat tgtacctgtg atgttgaaac cattgataga tttaataact
241acaggctttt cccaagacta caaaaacttc ttgaaagtga ctactttagg tattacaagg
301taaacctgaa gaggccgtgt cctttctgga atgacatcag ccagtgtgga agaagggact
361gtgctgtcaa accatgtcaa tctgatgaag ttcctgatgg aattaaatct gcgagctaca
421agtattctga agaagccaat aatctcattg aagaatgtga acaagctgaa cgacttggag
481cagtggatga atctctgagt gaggaaacac agaaggctgt tcttcagtgg accaagcatg
541atgattcttc agataacttc tgtgaagctg atgacattca gtcccctgaa gctgaatatg
601tagatttgct tcttaatcct gagcgctaca ctggttacaa gggaccagat gcttggaaaa
661tatggaatgt catctacgaa gaaaactgtt ttaagccaca gacaattaaa agacctttaa
721atcctttggc ttctggtcaa gggacaagtg aagagaacac tttttacagt tggctagaag
781gtctctgtgt agaaaaaaga gcattctaca gacttatatc tggcctacat gcaagcatta
841atgtgcattt gagtgcaaga tatcttttac aagagacctg gttagaaaag aaatggggac
901acaacattac agaatttcaa cagcgatttg atggaatttt gactgaagga gaaggtccaa
961gaaggcttaa gaacttgtat tttctctact taatagaact aagggcttta tccaaagtgt
1021taccattctt cgagcgccca gattttcaac tctttactgg aaataaaatt caggatgagg
1081aaaacaaaat gttacttctg gaaatacttc atgaaatcaa gtcatttcct ttgcattttg
1141atgagaattc attttttgct ggggataaaa aagaagcaca caaactaaag gaggactttc
1201gactgcattt tagaaatatt tcaagaatta tggattgtgt tggttgtttt aaatgtcgtc
1261tgtggggaaa gcttcagact cagggtttgg gcactgctct gaagatctta ttttctgaga
1321aattgatagc aaatatgcca gaaagtggac ctagttatga attccatcta accagacaag
1381aaatagtatc attattcaac gcatttggaa gaatttctac aagtgtgaaa gaattagaaa
1441acttcaggaa cttgttacag aatattcatt acccaacttt cttgtacaaa gtggttggta
1501agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1561ggtggcgcgt taagtcgaca atcaacctct ggatta

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