Construct: ORF ccsbBroad304_03151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011823.1_s304c1, BRDN0000408693
- Derived from:
- ccsbBroadEn_03151
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF8 (50717)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03151
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NM_015726.4 | 100% | 100% | |
| 2 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028106.1 | 46.3% | (many diffs) | |
| 3 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028104.2 | 43.4% | 1_459del;2251_4125del | |
| 4 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028103.2 | 42.6% | 1_533del;2325_4199del | |
| 5 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028105.1 | 41.8% | (many diffs) | |
| 6 | mouse | 98193 | Dcaf8 | DDB1 and CUL4 associated fa... | NM_153555.2 | 90.5% | 95.4% | (many diffs) |
| 7 | mouse | 98193 | Dcaf8 | DDB1 and CUL4 associated fa... | XM_006497065.3 | 90.5% | 95.4% | (many diffs) |
| 8 | mouse | 98193 | Dcaf8 | DDB1 and CUL4 associated fa... | XM_011238889.2 | 90.5% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccagcaaa gggagcagca cagatggcag aacagactta gctaatggaa 121 gcctgtctag cagtccagag gagatgtctg gagctgaaga ggggagggag acatcctcag 181 gcattgaagt ggaggcctca gacctgagtt tgagcttgac tggggatgat ggtggcccca 241 accgcaccag cacagaaagt cgaggcacag acacagagag ctcaggtgaa gataaggact 301 ctgacagcat ggaggacact ggtcattact ccattaatga tgaaaatcga gtccatgacc 361 gctcagagga agaggaagag gaggaagaag aggaggaaga agagcagcct cggcgccgtg 421 tacagcgcaa gcgggctaac cgtgaccagg actcatcaga tgatgagcgg gccctagagg 481 actgggtgtc ctcagaaaca tcagctctac cccgacctcg ctggcaagcc ctccctgccc 541 ttcgggagcg ggagctgggt tcaagtgccc gctttgtcta tgaggcctgt ggggcaagag 601 tctttgtgca gcgtttccgc ctgcagcatg ggcttgaggg ccatactggt tgtgtcaata 661 ccctgcactt taaccagcgc ggcacctggc tggccagtgg cagcgatgac ctgaaggtgg 721 tggtgtggga ttgggtacgg cggcagccag tactggactt tgagagtggc cacaaaagta 781 atgtgttcca ggccaagttt cttcctaaca gtggtgattc tactctggcc atgtgtgccc 841 gtgacgggca ggttcgagta gcagaactgt ctgccacaca gtgttgcaag aatacaaaac 901 gtgtggccca gcacaaggga gcgtcccaca agttggcact ggaaccagac tctccctgta 961 cgttcttatc tgcaggtgaa gatgcagttg ttttcaccat tgacctgaga caagaccgcc 1021 cagcgtcgaa actggtggtg acaaaagaga aagagaagaa agtggggctg tatacgatct 1081 atgtgaatcc tgccaatacc caccagtttg cagtgggtgg acgagatcag tttgtaagga 1141 tttatgacca gaggaaaatt gatgagaatg agaacaatgg agtactcaag aagttctgtc 1201 ctcatcacct ggtgaacagt gagtccaaag caaacatcac ctgtcttgtg tacagccacg 1261 acggcacaga gctcctggcc agttacaatg atgaagacat ttacctcttc aactcctctc 1321 acagtgatgg ggcccagtat gttaagagat acaagggcca cagaaataat gccacagtaa 1381 aaggcgtcaa tttctatggc cccaagagtg agtttgtggt gagcggtagt gactgtgggc 1441 acatcttcct ctgggagaaa tcatcctgcc agattattca gttcatggag ggggacaagg 1501 gaggcgtggt aaactgtctt gagccccacc ctcacctgcc tgtgctggca accagtggcc 1561 tagaccatga tgtgaagatc tgggcaccca cagctgaagc ttccactgag ctgacagggt 1621 taaaagatgt gattaagaag aacaagcggg agcgggatga agatagcttg caccaaactg 1681 acctgtttga tagtcacatg ctgtggttcc ttatgcatca cctgagacag agacgccatc 1741 accggcgctg gcgagaacct ggggttgggg ccacagacgc ggactctgat gagtctccca 1801 gctcctcaga cacatcggac gaggaggagg gccctgaccg ggtgcagtgc atgccatctt 1861 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1921 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1981 ggatta