Construct: ORF ccsbBroad304_03188
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002852.1_s304c1, BRDN0000406741
- Derived from:
- ccsbBroadEn_03188
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YARS2 (51067)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03188
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51067 | YARS2 | tyrosyl-tRNA synthetase 2 | NM_001040436.3 | 100% | 100% | |
| 2 | human | 51067 | YARS2 | tyrosyl-tRNA synthetase 2 | XR_242892.5 | 63.1% | 1_584del;2016_2265del | |
| 3 | human | 51067 | YARS2 | tyrosyl-tRNA synthetase 2 | XR_001748730.2 | 59.8% | 1_584del;2016_2390del | |
| 4 | human | 51067 | YARS2 | tyrosyl-tRNA synthetase 2 | XR_002957331.1 | 55.1% | 1_584del;2016_2595del | |
| 5 | human | 51067 | YARS2 | tyrosyl-tRNA synthetase 2 | XR_931296.3 | 46.1% | 1_584del;2016_3102del | |
| 6 | mouse | 70120 | Yars2 | tyrosyl-tRNA synthetase 2 (... | NM_198246.2 | 83% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1497
- ORF length:
- 1431
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcccatc ttgcggtcct tttcctgggg ccggtggtct ggtaccctaa 121 atctctcagt attgttgccc ttggggctgc gtaaggccca ctcgggcgct caggggttac 181 tggcagcgca gaaggctcga ggtctgttca aggacttctt cccggagacg gggacgaaaa 241 tagagctccc agagctcttc gaccgtggca cggcgagttt tccccaaacc atttactgtg 301 gcttcgaccc cacggcagac tcgcttcatg tgggtcatct acttgcgctg ctgggcctgt 361 ttcatttgca gcgagcgggc cacaacgtga tcgcgctggt gggaggcgcc acggcgcgcc 421 tgggagaccc gagcggccgt accaaggaac gcgaggcgct ggagacagag cgcgtgcgag 481 ccaacgcgcg agctctgcgc ctagggcttg aggccctggc ggctaatcac cagcagcttt 541 tcactgatgg gcgctcctgg ggcagcttca ctgtgctgga caactcggcc tggtaccaga 601 agcagcacct ggtggacttc ctggcggcag tggggggtca cttccgcatg gggacgctgc 661 tgagccggca gagcgtgcag ctgcggctca agagccccga gggcatgagc ttggccgagt 721 tcttttacca ggtgctccag gcctatgact tctattacct cttccagcgt tatggatgca 781 gggtccagct gggcggatct gatcaactag gcaacatcat gtccggatat gagttcatca 841 acaagttgac tggagaagat gtatttggaa tcaccgttcc tctaattaca agtacaactg 901 gagcaaagct gggaaagtct gctggcaacg ctgtttggct aaacagagat aagacatctc 961 catttgaatt gtatcaattc tttgtcaggc aaccggacga ttcagtggaa aggtacctga 1021 agctgttcac tttcctgccc cttccagaga ttgatcatat catgcagctg catgtcaaag 1081 agccagaaag gcggggtcct cagaaacgac tggcagcaga agtaacaaag cttgttcatg 1141 gacgagaagg attggattct gctaaaaggt gtacacaagc cctttatcac agtagcatag 1201 atgcactgga ggtcatgtct gatcaggagt taaaagagtt gtttaaagaa gctccatttt 1261 ctgaattttt tctcgatcct ggaacaagtg tcctagatac ttgccgcaaa gcaaatgcca 1321 ttccagatgg tccccgaggg tatcgaatga taacagaagg cggagtcagc ataaatcacc 1381 aacaagtaac aaatcctgag agtgttttaa ttgttggaca acatattctc aagaatggac 1441 tttccttact taaaatagga aaaagaaatt tctacattat aaaatggctt cagttgtgcc 1501 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1561 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1621 tta