Construct: ORF ccsbBroad304_03199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011548.1_s304c1, BRDN0000405273
- Derived from:
- ccsbBroadEn_03199
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNNI3K (51086)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03199
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51086 | TNNI3K | TNNI3 interacting kinase | NM_015978.3 | 100% | 100% | |
| 2 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001112808.2 | 87.5% | 86.7% | (many diffs) |
| 3 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001199327.1 | 76.4% | 73.2% | (many diffs) |
| 4 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | NM_177066.5 | 85% | 90.8% | (many diffs) |
| 5 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XR_375539.1 | 76.1% | (many diffs) | |
| 6 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240154.1 | 74% | 79.4% | (many diffs) |
| 7 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240155.1 | 74% | 79.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2574
- ORF length:
- 2505
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaattat aaatctagac caacccaaac ttgtactgat gaatggaaga 121 aaaaagtcag tgaatcatat gttatcacaa tagaaagatt agaagatgac ctgcagatca 181 aggaaaaaga actgacagaa ctaaggaata tatttggctc tgatgaagcc ttcagtaaag 241 tcaatttaaa ttaccgcact gaaaatgggc tgtctctact tcatttatgt tgcatttgtg 301 gaggcaagaa atcacatatt cgaactctta tgttgaaagg gctccgccca tctcgactga 361 caagaaatgg atttacagcc ttgcatttag cagtttacaa ggataatgca gaattgatca 421 cttctctgct tcacagtgga gctgatatac agcaggttgg atacggtggc ctcactgccc 481 tccatattgc tacaatagct ggccacctag aggctgctga tgtgctgttg caacatggag 541 ctaatgtcaa tattcaagat gcagtttttt tcactccatt gcatattgca gcgtactatg 601 gacatgaaca ggtaactcgc cttcttttga aatttggtgc tgatgtaaat gtaagtggtg 661 aagttggaga tagacccctc cacctagcat ctgcaaaagg attcttgaat attgcaaaac 721 tcttgatgga agaaggcagc aaagcagatg tgaatgctca agataatgaa gaccatgtcc 781 cactccattt ctgttctcga tttggacacc atgatatagt taagtatctg ctgcaaagtg 841 atttggaagt tcaacctcat gttgttaata tctatggaga taccccctta cacctggcat 901 gctacaatgg caaatttgaa gttgccaagg aaatcatcca aatatcagga acagaaagtc 961 tgactaagga aaacatcttc agtgaaacag cttttcatag tgcttgtacc tatggcaaga 1021 gcattgacct agtcaaattt cttcttgatc agaatgtcat aaacatcaac caccaaggaa 1081 gggatgggca cactggatta cactctgctt gctaccacgg tcacattcgc ctggttcagt 1141 tcttactgga taatggagct gatatgaatc tagtggcttg tgatcccagc aggtctagtg 1201 gtgaaaaaga tgagcagaca tgtttgatgt gggcttatga aaaagggcat gatgccattg 1261 tcacactcct gaagcattat aagagaccac aagatgaatt gccctgtaat gaatattctc 1321 agcctggagg agatggctcc tatgtgtctg ttccatcacc cttggggaag attaaaagca 1381 tgacaaaaga gaaggcagat attctcctcc taagagctgg attgccttca catttccatc 1441 ttcagctctc agaaattgag ttccatgaga ttattggctc aggttctttt gggaaagtat 1501 ataaaggacg atgcagaaat aaaatagtgg ctataaaacg ttatcgagcc aatacctact 1561 gctccaagtc agatgtggat atgttttgcc gagaggtgtc cattctctgc cagctcaatc 1621 atccctgcgt aattcagttt gtgggtgctt gcttgaatga tcccagccag tttgccattg 1681 tcactcaata catatcaggg ggttctctgt tctccctcct tcatgagcag aagaggattc 1741 ttgatttgca gtctaaatta attattgcag tagatgttgc caaaggcatg gagtaccttc 1801 acaacctgac acagccaatt atacatcgtg acttgaacag tcacaatatt cttctctatg 1861 aggatgggca tgctgtggtg gcagattttg gagaatcaag atttctacag tctctggatg 1921 aagacaacat gacaaaacaa cctgggaacc tccgttggat ggctcctgag gtgttcacgc 1981 agtgcactcg gtacaccatc aaagcagatg tcttcagcta tgctctgtgt ctgtgggaaa 2041 ttctcactgg cgaaattcca ttcgctcatc tcaagccagc ggctgcggca gcagacatgg 2101 cttaccacca catcagacct cccattggct attccattcc caagcccata tcatctctgc 2161 tgatacgagg gtggaacgca tgtcctgaag gaagacccga attttctgaa gttgtcatga 2221 agttagaaga gtgtctctgc aacattgagc tgatgtctcc tgcatcaagt aacagcagtg 2281 ggtctctctc accttcttct tcttctgatt gcctggtgaa ccggggagga cctggccgga 2341 gtcatgtggc agcattaaga agtcgtttcg aattggaata tgctctaaat gcaaggtcct 2401 atgctgcttt gtcccaaagt gctggacaat attcctctca aggtctgtct ttggaggaga 2461 tgaaaagaag tcttcaatac acacccattg acaaatatgg ctatgtatcc gatcccatga 2521 gctcaatgca ttttcattct tgccgaaata gtagcagctt tgaggacagc agcttgccaa 2581 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2641 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta