Construct: ORF ccsbBroad304_03199

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011548.1_s304c1, BRDN0000405273
Derived from:
ccsbBroadEn_03199
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TNNI3K (51086)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03199
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51086 TNNI3K TNNI3 interacting kinase NM_015978.3 100% 100%
2 human 100526835 FPGT-TNNI3K FPGT-TNNI3K readthrough NM_001112808.2 87.5% 86.7% (many diffs)
3 human 100526835 FPGT-TNNI3K FPGT-TNNI3K readthrough NM_001199327.1 76.4% 73.2% (many diffs)
4 mouse 435766 Tnni3k TNNI3 interacting kinase NM_177066.5 85% 90.8% (many diffs)
5 mouse 435766 Tnni3k TNNI3 interacting kinase XR_375539.1 76.1% (many diffs)
6 mouse 435766 Tnni3k TNNI3 interacting kinase XM_011240154.1 74% 79.4% (many diffs)
7 mouse 435766 Tnni3k TNNI3 interacting kinase XM_011240155.1 74% 79.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2574
ORF length:
2505
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggaaattat aaatctagac caacccaaac ttgtactgat gaatggaaga
121aaaaagtcag tgaatcatat gttatcacaa tagaaagatt agaagatgac ctgcagatca
181aggaaaaaga actgacagaa ctaaggaata tatttggctc tgatgaagcc ttcagtaaag
241tcaatttaaa ttaccgcact gaaaatgggc tgtctctact tcatttatgt tgcatttgtg
301gaggcaagaa atcacatatt cgaactctta tgttgaaagg gctccgccca tctcgactga
361caagaaatgg atttacagcc ttgcatttag cagtttacaa ggataatgca gaattgatca
421cttctctgct tcacagtgga gctgatatac agcaggttgg atacggtggc ctcactgccc
481tccatattgc tacaatagct ggccacctag aggctgctga tgtgctgttg caacatggag
541ctaatgtcaa tattcaagat gcagtttttt tcactccatt gcatattgca gcgtactatg
601gacatgaaca ggtaactcgc cttcttttga aatttggtgc tgatgtaaat gtaagtggtg
661aagttggaga tagacccctc cacctagcat ctgcaaaagg attcttgaat attgcaaaac
721tcttgatgga agaaggcagc aaagcagatg tgaatgctca agataatgaa gaccatgtcc
781cactccattt ctgttctcga tttggacacc atgatatagt taagtatctg ctgcaaagtg
841atttggaagt tcaacctcat gttgttaata tctatggaga taccccctta cacctggcat
901gctacaatgg caaatttgaa gttgccaagg aaatcatcca aatatcagga acagaaagtc
961tgactaagga aaacatcttc agtgaaacag cttttcatag tgcttgtacc tatggcaaga
1021gcattgacct agtcaaattt cttcttgatc agaatgtcat aaacatcaac caccaaggaa
1081gggatgggca cactggatta cactctgctt gctaccacgg tcacattcgc ctggttcagt
1141tcttactgga taatggagct gatatgaatc tagtggcttg tgatcccagc aggtctagtg
1201gtgaaaaaga tgagcagaca tgtttgatgt gggcttatga aaaagggcat gatgccattg
1261tcacactcct gaagcattat aagagaccac aagatgaatt gccctgtaat gaatattctc
1321agcctggagg agatggctcc tatgtgtctg ttccatcacc cttggggaag attaaaagca
1381tgacaaaaga gaaggcagat attctcctcc taagagctgg attgccttca catttccatc
1441ttcagctctc agaaattgag ttccatgaga ttattggctc aggttctttt gggaaagtat
1501ataaaggacg atgcagaaat aaaatagtgg ctataaaacg ttatcgagcc aatacctact
1561gctccaagtc agatgtggat atgttttgcc gagaggtgtc cattctctgc cagctcaatc
1621atccctgcgt aattcagttt gtgggtgctt gcttgaatga tcccagccag tttgccattg
1681tcactcaata catatcaggg ggttctctgt tctccctcct tcatgagcag aagaggattc
1741ttgatttgca gtctaaatta attattgcag tagatgttgc caaaggcatg gagtaccttc
1801acaacctgac acagccaatt atacatcgtg acttgaacag tcacaatatt cttctctatg
1861aggatgggca tgctgtggtg gcagattttg gagaatcaag atttctacag tctctggatg
1921aagacaacat gacaaaacaa cctgggaacc tccgttggat ggctcctgag gtgttcacgc
1981agtgcactcg gtacaccatc aaagcagatg tcttcagcta tgctctgtgt ctgtgggaaa
2041ttctcactgg cgaaattcca ttcgctcatc tcaagccagc ggctgcggca gcagacatgg
2101cttaccacca catcagacct cccattggct attccattcc caagcccata tcatctctgc
2161tgatacgagg gtggaacgca tgtcctgaag gaagacccga attttctgaa gttgtcatga
2221agttagaaga gtgtctctgc aacattgagc tgatgtctcc tgcatcaagt aacagcagtg
2281ggtctctctc accttcttct tcttctgatt gcctggtgaa ccggggagga cctggccgga
2341gtcatgtggc agcattaaga agtcgtttcg aattggaata tgctctaaat gcaaggtcct
2401atgctgcttt gtcccaaagt gctggacaat attcctctca aggtctgtct ttggaggaga
2461tgaaaagaag tcttcaatac acacccattg acaaatatgg ctatgtatcc gatcccatga
2521gctcaatgca ttttcattct tgccgaaata gtagcagctt tgaggacagc agcttgccaa
2581ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2641cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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