Construct: ORF ccsbBroad304_03402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000134.1_s304c1, BRDN0000407265
- Derived from:
- ccsbBroadEn_03402
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM11A (54065)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03402
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102723553 | SMIM11B | small integral membrane pro... | NM_001313692.1 | 100% | 100% | |
| 2 | human | 54065 | SMIM11A | small integral membrane pro... | NM_058182.4 | 100% | 100% | |
| 3 | human | 102723553 | SMIM11B | small integral membrane pro... | XM_017028204.1 | 100% | 100% | |
| 4 | human | 102723553 | SMIM11B | small integral membrane pro... | XM_017028205.1 | 100% | 100% | |
| 5 | human | 102723553 | SMIM11B | small integral membrane pro... | XR_002958589.1 | 14.3% | 1_154del;329_1209del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 240
- ORF length:
- 174
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttggaaggtt cttgagcacg tgcccctgct gctgtatatc ttggcagcaa 121 aaacattaat tctctgcctg acatttgctg gggtgaaaat gtatcaaaga aaaaggttgg 181 aggcaaaaca acaaaaactg gaggctgaaa ggaagaagca atcagagaaa aaagataact 241 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 301 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 361 ggatta