Construct: ORF ccsbBroad304_03446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006551.1_s304c1, BRDN0000408267
- Derived from:
- ccsbBroadEn_03446
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRN3 (54700)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03446
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54700 | RRN3 | RRN3 homolog, RNA polymeras... | NM_018427.4 | 100% | 100% | |
| 2 | human | 54700 | RRN3 | RRN3 homolog, RNA polymeras... | NM_001301064.1 | 95.3% | 95.3% | 252_253ins90 |
| 3 | human | 653390 | RRN3P2 | RRN3 homolog, RNA polymeras... | NR_003369.2 | 59.5% | (many diffs) | |
| 4 | human | 730092 | RRN3P1 | RRN3 homolog, RNA polymeras... | NR_003370.2 | 57.9% | (many diffs) | |
| 5 | mouse | 106298 | Rrn3 | RRN3 RNA polymerase I trans... | NM_001039521.1 | 85.1% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2022
- ORF length:
- 1953
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcaccg ctgcttcaca cgcgtttgcc gggagatgcg gccgcttcgt 121 cctctgcagt taagaagctg ggcgcgtcga ggactgggat ttcaaatatg cgtgcattag 181 agaatgactt tttcaattct cccccaagaa aaactgttcg gtttggtgga actgtgacag 241 aagtcttgct gaagtacaaa aagggtgaaa caaatgactt tgagttgttg aagaaccagc 301 tgttagatcc agacataaag gatgaccaga tcatcaactg gctgctagaa ttccgttctt 361 ctatcatgta cttgacaaaa gactttgagc aacttatcag tattatatta agattgcctt 421 ggttgaatag aagtcaaaca gtagtggaag agtatttggc ttttcttggt aatcttgtat 481 cagcacagac tgttttcctc agaccgtgtc tcagcatgat tgcttcccat tttgtgcctc 541 cccgagtgat cattaaggaa ggcgatgtag atgtttcaga ttctgatgat gaagatgata 601 atcttcctgc aaattttgac acatgtcaca gagccttgca aataatagca agatatgtac 661 catcgacacc gtggtttctc atgccaatac tggtggaaaa atttccattt gttcgaaaat 721 cagagagaac actggaatgt tacgttcata acttactaag gattagtgta tattttccaa 781 ccttgaggca tgaaattctg gagcttatta ttgaaaaact actcaagttg gatgtgaatg 841 catcccggca gggtattgaa gatgctgaag aaacagcaac tcaaacttgt ggtgggacag 901 attccacgga aggattgttt aatatggatg aagatgaaga aactgaacat gaaacaaagg 961 ctggtcctga acggctcgac cagatggtgc atcctgtagc cgagcgcctg gacatcctga 1021 tgtctttggt tttgtcctac atgaaggatg tctgctatgt agatggtaag gttgataacg 1081 gcaaaacaaa ggatctatat cgcgacctga taaacatctt tgacaaactc ctgttgccca 1141 cccatgcctc ctgccatgta cagtttttca tgttttacct ctgtagtttc aaattgggat 1201 tcgcagaggc atttttggaa catctctgga aaaaattgca ggacccaagt aatcctgcca 1261 tcatcaggca ggctgctgga aattatattg gaagcttttt ggcaagagct aaatttattc 1321 ctcttattac tgtaaaatca tgcctagatc ttttggttaa ctggctgcac atatacctta 1381 ataaccagga ttcgggaaca aaggcattct gcgatgttgc tctccatgga ccattttact 1441 cagcctgcca agctgtgttc tacacctttg tttttagaca caagcagctt ttgagcggaa 1501 acctgaaaga aggtttgcag tatcttcaga gtctgaattt tgagcggata gtgatgagcc 1561 agctaaatcc cctgaagatt tgcctgccct cagtggttaa cttttttgct gcaatcacaa 1621 ataagtacca gctcgtcttc tgctacacca tcattgagag gaacaatcgc cagatgctgc 1681 cagtcattag gagtaccgct ggaggagact cagtgcagat ctgcacaaac ccgctggaca 1741 ccttcttccc ctttgatccc tgtgtgctga agaggtcaaa gaaattcatt gatcctattt 1801 atcaggtgtg ggaagacatg agtgctgaag agctacagga gttcaagaaa cccatgaaaa 1861 aggacatagt ggaagatgaa gatgatgact ttctgaaagg cgaagtgccc cagaatgata 1921 ccgtgattgg gatcacacca agctcctttg acacgcattt ccgaagtcct tcaagtagtg 1981 tgggctcccc acccgtgttg tacatgcaac ccagtcccct cttgccaact ttcttgtaca 2041 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2101 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta