Construct: ORF ccsbBroad304_03519

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008740.1_s304c1, BRDN0000408334
Derived from:
ccsbBroadEn_03519
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC186 (55088)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03519
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55088 CCDC186 coiled-coil domain containi... NM_001321829.1 100% 100%
2 human 55088 CCDC186 coiled-coil domain containi... NM_018017.4 100% 100%
3 human 55088 CCDC186 coiled-coil domain containi... XM_011539915.3 72.4% 72.4% 0_1ins741
4 human 55088 CCDC186 coiled-coil domain containi... XM_011539916.3 64.2% 60.3% (many diffs)
5 human 55088 CCDC186 coiled-coil domain containi... NR_135815.1 33.2% (many diffs)
6 human 55088 CCDC186 coiled-coil domain containi... NM_153249.1 23.4% 23.4% 633_634ins2061
7 mouse 213993 Ccdc186 coiled-coil domain containi... XM_006526852.1 51% 54% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2763
ORF length:
2694
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcagagaca gaccacatag cctctacttc ctctgataaa aatgttggga
121aaacacctga attaaaggaa gactcatgca acttgttttc tggcaatgaa agcagcaaat
181tagaaaatga gtccaaacta ttgtcattaa acactgataa aactttatgt caacctaatg
241agcataataa tcgaattgaa gcccaggaaa attatattcc agatcatggt ggaggtgagg
301attcttgtgc caaaacagac acaggctcag aaaattctga acaaatagct aattttccta
361gtggaaattt tgctaaacat atttcaaaaa caaatgaaac agaacagaaa gtaacacaaa
421tattggtgga attaaggtca tctacatttc cagaatcagc taatgaaaag acttattcag
481aaagccccta tgatacagac tgcaccaaga aatttatttc aaaaataaag agcgtttcag
541catcagagga tttgttggaa gaaatagaat ctgagctctt atctacggag tttgcagaac
601atcgagtacc aaatggaatg aataagggag aacatgcatt agttctgttt gaaaagtgtg
661tgcaagataa atatttgcag caggaacata tcataaaaaa gttaattaaa gaaaataaga
721agcatcagga gctcttcgta gacatttgtt cagaaaaaga caatttaaga gaagaactaa
781agaaaagaac agaaactgag aagcagcata tgaacacaat taaacagtta gaatcaagaa
841tagaagaact taataaagaa gttaaagctt ccagagatca actaatagct caagacgtta
901cagctaaaaa tgcagttcag cagttacaca aagagatggc ccaacggatg gaacaggcca
961acaagaaatg tgaagaggca cgccaagaaa aagaagcaat ggtaatgaaa tatgtaagag
1021gtgagaagga atctttagat cttcgaaagg aaaaagagac acttgagaaa aaacttagag
1081atgcaaataa ggaacttgag aaaaacacta acaaaattaa gcagctttct caggagaaag
1141gacggttgca ccagctgtat gaaactaagg aaggcgaaac gactagactc atcagagaaa
1201tagacaaatt aaaggaagac attaactctc acgtcatcaa agtaaagtgg gcacaaaaca
1261aattaaaagc tgaaatggat tcacacaagg aaaccaaaga taaactcaaa gaaacaacaa
1321caaaattaac acaagcaaag gaagaagcag atcagatacg aaaaaactgt caggatatga
1381taaaaacata tcaggagtca gaagaaatta aatcaaatga gcttgatgca aagcttagag
1441tcacaaaagg agaacttgaa aaacaaatgc aagaaaaatc tgaccagcta gagatgcatc
1501atgccaaaat aaaggaacta gaagatctga agagaacatt taaggagggt atggatgagt
1561taagaacact gagaacaaag gtgaaatgtc tagaagatga acgattaaga acagaagatg
1621aattatcaaa atataaggaa attattaatc gccaaaaagc tgaaattcag aatttattgg
1681acaaggtgaa aactgcagat cagctacagg agcagcttca aagaggtaag caagaaattg
1741aaaatttgaa agaagaagtg gaaagtctta attctttgat taatgaccta caaaaagaca
1801tcgaaggcag taggaaaaga gaatctgagc tgctgctgtt tacagaaagg ctcactagta
1861agaatgcaca gcttcagtct gaatccaatt ctttgcagtc acaatttgat aaagtttcct
1921gtagtgaaag tcagttacaa agccagtgtg aacaaatgaa acagacaaat attaatttgg
1981aaagtaggtt gttgaaagag gaagaactgc gaaaagagga agtccaaact ctgcaagctg
2041aactcgcttg tagacaaaca gaagttaaag cattgagtac ccaggtagaa gaattaaaag
2101atgagttagt aactcagaga cgtaaacatg cctctagtat caaggatctc accaaacaac
2161ttcagcaagc acgaagaaaa ttagatcagg ttgagagtgg aagctatgac aaagaagtca
2221gcagcatggg aagtcgttct agttcatcag ggtccctgaa tgctcgaagc agtgcagaag
2281atcgatctcc agaaaatact gggtcctcag tagctgtgga taactttcca caagtagata
2341aggccatgtt gattgagaga atagttaggc tgcaaaaagc acatgcccgg aaaaatgaaa
2401agatagaatt tatggaggac cacatcaaac aactggtgga agaaattagg aaaaaaacaa
2461aaataattca aagttatatt ttacgagaag aatcaggcac actttcttca gaggcatctg
2521attttaacaa agttcattta agtagacggg gtggcatcat ggcatcttta tatacatccc
2581atccagctga caatggatta acattggagc tctctttgga aatcaaccga aaattacagg
2641ctgttttgga ggatacgtta ctaaaaaata ttactttgaa ggaaaatcta caaacacttg
2701gaacagaaat agaacgtctt attaaacacc agcatgaact agaacagagg acaaagaaaa
2761ccttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2821tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2881tctggatta

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