Construct: ORF ccsbBroad304_03529
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012079.1_s304c1, BRDN0000399231
- Derived from:
- ccsbBroadEn_03529
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIWIL2 (55124)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03529
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55124 | PIWIL2 | piwi like RNA-mediated gene... | NM_001135721.2 | 100% | 100% | |
| 2 | human | 55124 | PIWIL2 | piwi like RNA-mediated gene... | NM_018068.5 | 100% | 100% | |
| 3 | human | 55124 | PIWIL2 | piwi like RNA-mediated gene... | NM_001330480.2 | 96.3% | 96.3% | 2656_2657ins108 |
| 4 | human | 55124 | PIWIL2 | piwi like RNA-mediated gene... | XM_005273551.4 | 82.5% | 82.3% | (many diffs) |
| 5 | mouse | 57746 | Piwil2 | piwi-like RNA-mediated gene... | NM_021308.1 | 85.5% | 87.9% | (many diffs) |
| 6 | mouse | 57746 | Piwil2 | piwi-like RNA-mediated gene... | XM_006519329.3 | 85.5% | 87.9% | (many diffs) |
| 7 | mouse | 57746 | Piwil2 | piwi-like RNA-mediated gene... | XM_006519328.1 | 79.7% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2985
- ORF length:
- 2919
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tcctttccga ccatcgttca ggggccagtc tcctatccac ccatcccagt 121 gccaggctgt acggatgcca ggctgttggc cacaagcttc taaacctttg gacccagctc 181 tgggcagggg agcacctgca ggcagaggcc atgtatttgg aaagccagag gaaccaagca 241 cacagagggg gccagcacaa agggagtctg tgggtttggt ctccatgttc cgaggcctgg 301 gcattgaaac agtttctaag acccctctga aacgggaaat gcttccatca ggtagaggca 361 ttttaggtcg aggcttgtct gctaatctgg tacgcaagga cagggaggaa ctctctccca 421 ctttttggga tccaaaagtg ttggcggctg gggacagcaa gatggcagag acctccgttg 481 gttggagtag gacgcttgga agagggagtt cagatgcgtc tttattacca ctgggaagag 541 cagcaggtgg tatcagcaga gaagtggaca agcctccctg taccttcagc acaccgtccc 601 ggggtccccc gcagctgtca tcaccaccag ctctgcccca gtctcccctg cactctccag 661 atcgccctct ggtcctgact gtggaacaca aggaaaaaga gcttattgtg aagcaaggat 721 caaaaggaac acctcagtct ttgggactga acctcgtcaa aatacagtgt cataatgaag 781 cagtttatca atatcatgtg actttcagcc ccaatgtgga gtgcaaaagc atgaggttcg 841 gcatgttgaa ggaccatcaa gctgtcaccg gcaacgtcac tgcgtttgat ggatctattc 901 tctatctgcc tgttaagctt caacaagttc ttgagttaaa aagtcaaagg aaaacagaca 961 gtgctgaaat cagcattaag attcagatga caaagatcct ggagccctgc tctgacctgt 1021 gcattccctt ctacaatgtt gttttccgtc gggtaatgaa acttttagat atgaagcttg 1081 tggggagaaa cttttatgac cctacaagtg ctatggtact acagcaacac agattgcaga 1141 tctggccagg ctatgcagct agcatccgaa ggacagatgg agggctcttc ctgctagctg 1201 atgtctccca taaggtcatt cggaatgact gtgtgctgga tgtcatgcat gccatttatc 1261 agcagaataa agaacacttc caggatgagt gtactaagct tctggttggc aatattgtta 1321 tcacccgata taacaatcgt acctatcgta ttgatgatgt ggattggaat aagactccaa 1381 aggatagctt cacgatgtct gatgggaaag agatcacatt cttggaatac tacagcaaaa 1441 attatgggat cacagttaag gaagaggacc agccattgct gattcacagg cccagtgaga 1501 gacaggataa tcatgggatg ctgctaaaag gggaaatcct gctgctgcct gagctttctt 1561 ttatgaccgg aatcccagag aagatgaaga aggacttcag agccatgaag gatttggctc 1621 agcaaatcaa tctgagcccc aagcaacacc atagtgcttt ggaatgcttg ctgcaaagaa 1681 ttgcaaagaa cgaggcagcc accaatgaac tgatgcgttg ggggctccgt ctgcaaaagg 1741 atgtacataa gattgaagga cgtgttctgc caatggaaag aattaactta aaaaatactt 1801 cgtttatcac atctcaggaa ctaaactggg ttaaggaagt aaccagagac ccttccatct 1861 tgactatccc catgcatttc tgggcacttt tttacccaaa gagagcaatg gaccaggctc 1921 gagaactggt caacatgttg gagaagatag ccggccccat tggcatgcgt atgagcccac 1981 cggcctgggt tgaactaaag gatgaccgaa tagagactta tgtcagaacc attcaatcca 2041 cgttaggagc tgaggggaag atacagatgg ttgtttgcat catcatgggc ccacgtgatg 2101 atctctatgg ggccatcaag aagctgtgct gtgtgcagtc cccagtgccc tcccaggttg 2161 tcaatgttcg aaccattggt cagcccacca ggcttcggag tgtggcccag aagattttac 2221 ttcagattaa ctgtaaattg ggtggtgagc tctggggagt ggatattcct ctgaaacagt 2281 taatggtgat cgggatggat gtttaccatg accccagtag aggcatgcgc tccgtggttg 2341 gcttcgtggc aagcatcaat ctcaccctca caaaatggta ttcccgggtg gtgttccaga 2401 tgccgcatca ggagattgtg gacagcctga agctatgcct cgtgggctcc ttaaaaaagt 2461 tttatgaggt gaaccactgt ctaccagaga agattgtggt gtaccgtgat ggagtgtctg 2521 atggccaact gaagacagtt gccaactatg agattcctca actacagaag tgttttgaag 2581 cttttgagaa ttatcagccc aagatggtgg tgtttgtagt tcagaagaaa atcagtacta 2641 atctatatct ggctgctcct cagaactttg taactcccac tcctggaact gtggtagatc 2701 atacaataac aagctgtgag tgggtggatt tctatcttct tgcccatcat gtacggcagg 2761 gctgtggcat tcctacgcat tatgtctgtg ttctcaacac cgcaaacctg agccctgatc 2821 atatgcagag gctgactttc aaactgtgcc acatgtactg gaattggcct ggcaccatca 2881 gagttccagc tccttgcaag tatgcccaca agctagcttt cctgtcagga cacatcttgc 2941 atcatgaacc agccatccag ctgtgcgaga acctgttctt cctgtgccca actttcttgt 3001 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 3061 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a