Transcript: Human XM_005273551.4

PREDICTED: Homo sapiens piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PIWIL2 (55124)
Length:
2699
CDS:
132..2546

Additional Resources:

NCBI RefSeq record:
XM_005273551.4
NBCI Gene record:
PIWIL2 (55124)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005273551.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356470 TATTGTTATCACCCGATATAA pLKO_005 1379 CDS 100% 15.000 21.000 N PIWIL2 n/a
2 TRCN0000231012 ACCGGCCTGGGTTGAACTAAA pLKO_005 2045 CDS 100% 13.200 18.480 N LOC652554 n/a
3 TRCN0000007883 CGATATAACAATCGTACCTAT pLKO.1 1392 CDS 100% 4.950 6.930 N PIWIL2 n/a
4 TRCN0000007884 CGTCTTTATTACCACTGGGAA pLKO.1 583 CDS 100% 2.640 3.696 N PIWIL2 n/a
5 TRCN0000218539 GATGACCGAATAGAGACTTAT pLKO_005 2067 CDS 100% 13.200 10.560 N LOC652554 n/a
6 TRCN0000356548 ACTGCGTTTGATGGATCTATT pLKO_005 945 CDS 100% 13.200 9.240 N PIWIL2 n/a
7 TRCN0000356549 GGGAGTTCAGATGCGTCTTTA pLKO_005 570 CDS 100% 13.200 9.240 N PIWIL2 n/a
8 TRCN0000231013 GGTGATCGGGATGGATGTTTA pLKO_005 2351 CDS 100% 13.200 9.240 N LOC652554 n/a
9 TRCN0000217971 GAAGATACAGATGGTTGTTTG pLKO_005 2123 CDS 100% 10.800 7.560 N LOC652554 n/a
10 TRCN0000007885 GCCAATGGAAAGAATTAACTT pLKO.1 1835 CDS 100% 5.625 3.938 N PIWIL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005273551.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03529 pDONR223 100% 82.5% 82.3% None (many diffs) n/a
2 ccsbBroad304_03529 pLX_304 0% 82.5% 82.3% V5 (many diffs) n/a
3 TRCN0000469723 TTCATGCCAGTACTAGGCCGATGT pLX_317 .5% 82.5% 82.3% V5 (many diffs) n/a
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