Construct: ORF ccsbBroad304_03550

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013153.1_s304c1, BRDN0000406898
Derived from:
ccsbBroadEn_03550
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BBS7 (55212)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03550
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55212 BBS7 Bardet-Biedl syndrome 7 NM_018190.3 100% 100%
2 human 55212 BBS7 Bardet-Biedl syndrome 7 NM_176824.3 93.9% 93.9% 2016_2145delinsT
3 human 55212 BBS7 Bardet-Biedl syndrome 7 XM_005263106.4 93.8% 93.8% 601_603delGTA;2019_2148delinsT
4 human 55212 BBS7 Bardet-Biedl syndrome 7 XM_011532080.3 92% 92% 602_646del;2061_2190delinsT
5 human 55212 BBS7 Bardet-Biedl syndrome 7 XM_011532079.3 91.8% 91.9% 601_648del;2064_2193delinsT
6 human 55212 BBS7 Bardet-Biedl syndrome 7 XM_017008358.2 91.6% 91.6% 601_603delGTA;1510_1511ins165
7 human 55212 BBS7 Bardet-Biedl syndrome 7 XM_017008357.2 86.2% 86.2% 1507_1508ins165;1851_1980delinsT
8 human 55212 BBS7 Bardet-Biedl syndrome 7 XM_011532081.3 84.3% 84.4% 601_648del;1555_1556ins165;1899_2028delinsT
9 mouse 71492 Bbs7 Bardet-Biedl syndrome 7 (hu... NM_027810.3 82.4% 86% (many diffs)
10 mouse 71492 Bbs7 Bardet-Biedl syndrome 7 (hu... XM_006535535.3 80% 83.3% (many diffs)
11 mouse 71492 Bbs7 Bardet-Biedl syndrome 7 (hu... XM_006535536.3 60.7% 63.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2082
ORF length:
2016
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tctgatttta aaccgaatgg attatctgca ggtgggagta acatctcaga
121agactatgaa gctaattcct gcctcaagac acagagctac acaaaaggtg gttattggag
181atcatgatgg ggtagttatg tgctttggca tgaagaaagg agaagcagca gcagtgttca
241agactttacc cgggccgaag attgcaaggc tggaactggg aggggttatc aacacacctc
301aggagaaaat ttttattgct gcagcatctg agattagagg cttcacaaaa agaggaaaac
361agttcctctc ctttgaaaca aacctcactg aaagcattaa agctatgcac atatctggct
421cagacctctt tctcagtgca agttacatct ataaccatta ttgtgactgc aaagaccaac
481attattacct ttctggggat aaaatcaatg atgtgatctg ccttccagtg gaaagattat
541ctcgtatcac acctgtattg gcctgccagg acagagtgct cagagtttta cagggatctg
601atgtgatgta tgcagttgaa gttcctggac cccctactgt cttagcacta cacaatggaa
661atggcggtga ctctggagaa gaccttttgt ttgggacatc agacggaaaa cttgcgctta
721tacagattac tacatccaaa ccagtacgca agtgggaaat tcaaaatgag aaaaagagag
781gaggtatttt gtgtattgac agctttgaca ttgtgggtga tggggttaaa gatttacttg
841ttgggagaga tgacggaatg gtggaagtgt atagttttga taatgcaaat gaacctgttc
901tacgatttga tcagatgttg tctgaaagcg tcacatctat ccagggtggt tgtgtaggaa
961aagacagcta tgatgaaatc gtggtgtcca catattcagg ctgggttaca ggtctgacaa
1021cagagcccat tcataaggaa agtggaccag gagaagaact aaaaattaat caggagatgc
1081agaataaaat ttcttcctta cggaatgagt tggaacattt gcagtataag gtattgcagg
1141aaagagagaa ttatcaacag tcttctcaat caagcaaagc aaaatcagca gtaccttcct
1201ttggtataaa tgataaattt acactaaata aagatgatgc cagttacagc cttatcttag
1261aggtacagac tgcaatagat aatgtcttaa tacagagtga tgttccaata gatttacttg
1321atgtggataa aaattctgct gttgttagct ttagcagctg tgattctgag tcaaacgaca
1381acttccttct tgccacttat cggtgccagg cagatactac aaggctggaa ctcaagattc
1441gctcaattga aggccagtat ggcacactac aagcatatgt gactccaaga attcaaccca
1501aaacctgtca ggtccgccag taccacatca aacctctttc actccatcaa agaactcact
1561ttattgatca tgacagaccc atgaatacac tgaccctaac aggccagttc agttttgctg
1621aagttcactc ctgggtggtt ttttgtctgc ctgaagttcc agaaaaacct ccagcaggag
1681aatgtgtgac attttacttt cagaacacct ttctagatac acaacttgaa agtacctaca
1741gaaaaggaga gggagttttt aaatctgaca acatttctac tatctccatc ctaaaagatg
1801tgctttctaa agaagctaca aaaaggaaaa ttaacctcaa catatcatac gagataaatg
1861aagtatcagt caaacacact ttaaagctaa tccacccaaa gctggagtac cagttgcttt
1921tggctaagaa agtgcagtta attgatgctt taaaagaatt acagattcat gagggaaata
1981cgaactttct gataccagaa tatcactgta ttctagaaga ggcagatcac ctacaggaag
2041aatacaaaaa gcaacctgca catcttgaaa gactctatgg ttacccaact ttcttgtaca
2101aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2161ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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