Construct: ORF ccsbBroad304_03559

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009122.1_s304c1, BRDN0000401524
Derived from:
ccsbBroadEn_03559
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIRREL1 (55243)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03559
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55243 KIRREL1 kirre like nephrin family a... NM_018240.7 100% 100%
2 human 55243 KIRREL1 kirre like nephrin family a... XM_005245305.5 97.9% 97.9% 1044_1091del
3 human 55243 KIRREL1 kirre like nephrin family a... NM_001286349.1 86.7% 86.6% 52_53ins300
4 mouse 170643 Kirrel kin of IRRE like (Drosophila) XM_006501076.3 86.1% 93.9% (many diffs)
5 mouse 170643 Kirrel kin of IRRE like (Drosophila) NM_001170985.1 84.6% 90.9% (many diffs)
6 mouse 170643 Kirrel kin of IRRE like (Drosophila) NM_130867.3 84.6% 90.9% (many diffs)
7 mouse 170643 Kirrel kin of IRRE like (Drosophila) XM_011240028.2 82.9% 89.1% (many diffs)
8 mouse 170643 Kirrel kin of IRRE like (Drosophila) XM_011240029.1 82.9% 89.1% (many diffs)
9 mouse 170643 Kirrel kin of IRRE like (Drosophila) XM_017319471.1 79.6% 87% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2340
ORF length:
2271
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gctgagcctc ctcgtctgga tcctcactct ctccgatact ttctcccaag
121ggacccagac ccgcttcagc caggagccag ctgaccagac ggtggtggct ggacagcggg
181ccgtgctccc ctgtgtgctg ctcaactact ctggaattgt gcaatggacc aaggacgggc
241tggccctggg catgggccag ggcctcaaag cctggccacg gtaccgggtt gtgggctccg
301cagacgctgg gcagtacaac ctggagatca cagatgctga gctctctgac gacgcctctt
361acgagtgcca ggccacggag gccgccctgc gctctcggcg ggccaaactc accgtgctca
421tccccccaga ggacaccagg attgacggag gccctgtgat tctactgcag gcaggcaccc
481cccacaacct cacatgccgg gccttcaatg cgaagcctgc tgccaccatc atctggttcc
541gggacgggac gcagcaggag ggcgctgtgg ccagcacgga attgctgaag gatgggaaga
601gggagaccac cgtgagccaa ctgcttatta accccacgga cctggacata gggcgtgtct
661tcacttgccg aagcatgaac gaagccatcc ctagtggcaa ggagacttcc atcgagctgg
721atgtgcacca ccctcctaca gtgaccctgt ccattgagcc acagacggtg caggagggtg
781agcgtgttgt ctttacctgc caggccacag ccaaccccga gatcttgggc tacaggtggg
841ccaaaggggg tttcttgatt gaagacgccc acgagagtcg ctatgagaca aatgtggatt
901attccttttt cacggagcct gtgtcttgtg aggttcacaa caaagtggga agcaccaatg
961tcagcacttt agtaaatgtc cactttgctc cccggattgt agttgacccc aaacccacaa
1021ccacagacat tggctctgat gtgaccctta cctgtgtctg ggttgggaat ccccccctca
1081ctctcacctg gaccaaaaag gactcaaata tggtcctgag taacagcaac cagctgctgc
1141tgaagtcggt gactcaggca gacgctggca cctacacctg ccgggccatc gtgcctcgaa
1201tcggagtggc tgagcgggag gtgccgctct atgtgaacgg gccccccatc atctccagtg
1261aggcagtgca gtatgctgtg aggggtgacg gtggcaaggt ggagtgtttc attgggagca
1321caccaccccc agaccgcata gcatgggcct ggaaggagaa cttcttggag gtggggaccc
1381tggaacgcta tacagtggag aggaccaact caggcagtgg ggtgctatcc acgctcacca
1441tcaacaatgt catggaggcc gactttcaga ctcactacaa ctgcaccgcc tggaacagct
1501tcgggccagg cacagccatc atccagctgg aagagcgaga ggtgttacct gtgggcatca
1561tagctggggc caccatcggc gcgagcatcc tgctcatctt cttcttcatc gccttggtat
1621tcttcctcta ccggcgccgc aaaggcagtc gcaaagacgt gaccctgagg aagctggata
1681tcaaggtgga gacagtgaac cgagagccac ttacgatgca ttctgaccgg gaggatgaca
1741ccgccagcgt ctccacagca acccgggtca tgaaggccat ctactcgtcg tttaaggatg
1801atgtggatct gaagcaggac ctgcgctgcg acaccatcga cacccgggag gagtatgaga
1861tgaaggaccc caccaatggc tactacaacg tgcgtgccca tgaagaccgc ccgtcttcca
1921gggcagtgct ctatgctgac taccgtgccc ctggccctgc ccgcttcgac ggccgcccct
1981catcccgtct ctcccactcc agcggctatg cccagctcaa cacctatagc cggggccctg
2041cctctgacta tggccctgag cccacacccc ctggccctgc tgccccagct ggcactgaca
2101caaccagcca gctgtcctac gagaactatg agaagttcaa ctcccatccc ttccctgggg
2161cagctgggta ccccacctac cgactgggct acccccaggc cccaccctct ggcctggagc
2221ggaccccata tgaggcgtat gaccccattg gcaagtacgc cacagccact cgattctcct
2281acacctccca gcactcggac tacggccagc gattccagca gcgcatgcag actcacgtgt
2341tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
2401attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
2461ggatta

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