Construct: ORF ccsbBroad304_03600

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010551.1_s304c1, BRDN0000411704
Derived from:
ccsbBroadEn_03600
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMPD3 (55512)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03600
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55512 SMPD3 sphingomyelin phosphodieste... NM_018667.4 100% 100%
2 human 55512 SMPD3 sphingomyelin phosphodieste... XM_005256031.4 100% 100%
3 human 55512 SMPD3 sphingomyelin phosphodieste... XM_005256032.3 100% 100%
4 human 55512 SMPD3 sphingomyelin phosphodieste... XM_011523207.1 100% 100%
5 human 55512 SMPD3 sphingomyelin phosphodieste... XM_011523208.2 100% 100%
6 human 55512 SMPD3 sphingomyelin phosphodieste... XM_011523209.2 100% 100%
7 human 55512 SMPD3 sphingomyelin phosphodieste... XM_017023405.1 100% 100%
8 human 55512 SMPD3 sphingomyelin phosphodieste... XM_017023406.1 100% 100%
9 human 55512 SMPD3 sphingomyelin phosphodieste... XM_017023407.1 100% 100%
10 human 55512 SMPD3 sphingomyelin phosphodieste... XM_017023408.1 100% 100%
11 human 55512 SMPD3 sphingomyelin phosphodieste... XM_011523210.2 70.7% 67.6% (many diffs)
12 human 55512 SMPD3 sphingomyelin phosphodieste... XR_933371.2 66.3% (many diffs)
13 mouse 58994 Smpd3 sphingomyelin phosphodieste... NM_021491.3 86.5% 91% (many diffs)
14 mouse 58994 Smpd3 sphingomyelin phosphodieste... XM_006531241.3 86.5% 91% (many diffs)
15 mouse 58994 Smpd3 sphingomyelin phosphodieste... XM_011248462.1 86.5% 91% (many diffs)
16 mouse 58994 Smpd3 sphingomyelin phosphodieste... XM_011248463.2 86.5% 91% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2034
ORF length:
1965
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggttttgtac acgaccccct ttcctaacag ctgtctgtcc gccctgcact
121gtgtgtcctg ggcccttatc tttccatgct actggctggt ggaccggctc gctgcctcct
181tcatacccac cacctacgag aagcgccagc gggcagacga cccgtgctgc ctgcagctgc
241tctgcactgc cctcttcacg cccatctacc tggccctcct ggtggcctcg ctgccctttg
301cgtttctcgg ctttctcttc tggtccccac tgcagtcggc ccgccggccc tacatctatt
361cacggctgga agacaagggc ctggccggtg gggcagccct gctcagtgaa tggaagggca
421cggggcctgg caaaagcttc tgctttgcca ctgccaacgt ctgcctcctg cccgactcac
481tcgccagggt caacaacctt tttaacaccc aagcgcgggc caaggagatc gggcagagaa
541tccgcaatgg ggccgcccgg ccccagatca aaatttacat cgactccccc accaatacct
601ccatcagcgc cgctagcttc agcagcctgg tgtcaccaca gggcggcgat ggggtggccc
661gggccgtccc cgggagcatt aagaggacag cctctgtgga gtacaagggt gacggtgggc
721ggcaccccgg tgacgaggct gccaacggcc cagcctctgg ggaccctgtc gacagcagca
781gcccggagga tgcctgcatc gtgcgcatcg gtggcgagga gggcggccgg ccacctgaag
841ctgacgaccc tgtgcctggg ggccaggcca ggaacggagc tggcgggggc ccaaggggcc
901agacgcccaa ccataatcag caggacgggg attcagggag cctgggcagc ccctcggcct
961cccgggagtc cctggtgaag gggcgagctg ggccagacac cagtgccagc ggggagccag
1021gtgccaacag caagctcctg tacaaggcct cggtggtgaa gaaggcggct gcacgcagga
1081ggcggcaccc cgacgaggcc ttcgaccatg aggtctccgc cttcttcccc gccaacctgg
1141acttcctgtg cctgcaggag gtgtttgaca agcgagcagc caccaaattg aaagagcagc
1201tgcacggcta cttcgagtac atcctgtacg acgtcggggt ctacggctgc cagggctgct
1261gcagcttcaa gtgtctcaac agcggcctcc tctttgccag ccgctacccc atcatggacg
1321tggcctatca ctgttacccc aacaagtgta acgacgatgc cctggcctct aagggagctc
1381tgtttctcaa ggtgcaggtg ggaagcacac ctcaggacca aagaatcgtc gggtacatcg
1441cctgcacaca cctgcatgcc ccgcaagagg acagcgccat ccggtgtggg cagctggacc
1501tgcttcagga ctggctggct gatttccgaa aatctacctc ctcgtccagc gcagccaacc
1561ccgaggagct ggtggcattt gacgtcgtct gtggagattt caactttgat aactgctcct
1621ctgacgacaa gctggagcag caacactccc tgttcaccca ctacagggac ccctgccgcc
1681tggggcctgg tgaggagaag ccgtgggcca tcggtactct gctggacacg aacggcctgt
1741acgatgagga tgtgtgcacc cccgacaacc tgcagaaggt cctggagagt gaggagggcc
1801gcagggagta cctggcgttt cccaccagca agagctcggg ccagaagggg cggaaggagc
1861tgctgaaggg caacggccgg cgcatcgact acatgctgca tgcagaggag gggctgtgcc
1921cagactggaa ggccgaggtg gaagaattca gttttatcac ccagctgtcc ggcctgacgg
1981accacctgcc agtagccatg cgactgatgg tgtcttcggg ggaggaggag gcattgccaa
2041ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2101cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

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