Construct: ORF ccsbBroad304_03642
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011451.1_s304c1, BRDN0000406828
- Derived from:
- ccsbBroadEn_03642
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM222B (55731)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03642
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55731 | FAM222B | family with sequence simila... | NM_001077498.3 | 100% | 100% | |
| 2 | human | 55731 | FAM222B | family with sequence simila... | NM_001288632.2 | 100% | 100% | |
| 3 | human | 55731 | FAM222B | family with sequence simila... | NM_001288633.2 | 100% | 100% | |
| 4 | human | 55731 | FAM222B | family with sequence simila... | NM_001288634.2 | 100% | 100% | |
| 5 | human | 55731 | FAM222B | family with sequence simila... | NM_001288635.2 | 100% | 100% | |
| 6 | human | 55731 | FAM222B | family with sequence simila... | NM_018182.4 | 100% | 100% | |
| 7 | human | 55731 | FAM222B | family with sequence simila... | NM_001288631.2 | 96.2% | 94.4% | (many diffs) |
| 8 | human | 55731 | FAM222B | family with sequence simila... | NM_001288636.2 | 77.2% | 77.2% | 0_1ins384 |
| 9 | human | 55731 | FAM222B | family with sequence simila... | NM_001288637.2 | 77.2% | 77.2% | 0_1ins384 |
| 10 | human | 55731 | FAM222B | family with sequence simila... | NM_001288638.2 | 77.2% | 77.2% | 0_1ins384 |
| 11 | human | 55731 | FAM222B | family with sequence simila... | NM_001288639.2 | 77.2% | 77.2% | 0_1ins384 |
| 12 | human | 55731 | FAM222B | family with sequence simila... | NM_001288640.2 | 77.2% | 77.2% | 0_1ins384 |
| 13 | human | 55731 | FAM222B | family with sequence simila... | XM_011524986.2 | 77.2% | 77.2% | 0_1ins384 |
| 14 | human | 55731 | FAM222B | family with sequence simila... | XM_011524987.1 | 77.2% | 77.2% | 0_1ins384 |
| 15 | human | 55731 | FAM222B | family with sequence simila... | XM_011524988.2 | 77.2% | 77.2% | 0_1ins384 |
| 16 | human | 55731 | FAM222B | family with sequence simila... | XM_017024835.2 | 77.2% | 77.2% | 0_1ins384 |
| 17 | mouse | 216971 | Fam222b | family with sequence simila... | NM_001364110.1 | 95.4% | 97.3% | (many diffs) |
| 18 | mouse | 216971 | Fam222b | family with sequence simila... | NM_145430.3 | 95.4% | 97.3% | (many diffs) |
| 19 | mouse | 216971 | Fam222b | family with sequence simila... | NM_001346762.2 | 73.1% | 74.9% | (many diffs) |
| 20 | mouse | 216971 | Fam222b | family with sequence simila... | XM_030245839.1 | 73.1% | 74.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1752
- ORF length:
- 1686
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct agcctgtcta ccagggccag gtgacctgtc ctttcagctt ctttctcaca 121 cgcagatgaa cactggactt cagaaatggg acactacaca gaaaatgaga actgctcact 181 atcctacccc agccgaattg gatgcgtatg ctaagaaggt cgcaaacaac ccactgacta 241 taaaaatctt ccccaacagt gtgaaggttc cccagcggaa acacgttcgt cgtactgtga 301 acggcctcga cacatcagcc cagcgctaca gcccctaccc gacacaggct gccaccaagg 361 caggcctgct tgccattgtc aaagtgccag ccaaaagcat actcaaggac tttgacggca 421 cccgagcccg gttgctccct gaggccatca tgaacccccc agtggcaccc tatgctactg 481 tggcacccag cactttagcc cacccccagg cccaggctct ggcccgccag caggccctgc 541 agcatgcaca gaccctggcc catgcccctc cccagacgct gcagcaccct cagggtatcc 601 cgccacccca ggcactgtcc caccctcaga gcctccagca gcctcagggc ctgggccacc 661 ctcagcccat ggcccaaacc cagggcttgg tccaccctca ggccctggct caccagggtc 721 tccagcaccc ccacaatccc ttgctgcatg gaggccggaa gatgccagac tcagatgccc 781 ccccgaatgt gaccgtgtct acctcaacta tccccctttc aatggcggcc actctgcagc 841 acagccagcc tccggacctg agtagcatcg tgcaccagat caaccagttt tgccagacga 901 gggcaggcat cagcactacc tcagtgtgtg agggccagat cgccaacccc agccccatta 961 gtcgcagtct gctcatcaat gcaagcaccc gggtgtcgac ccacagcgtc cccacaccaa 1021 tgccttcatg tgtggtcaat cccatggagc acacccacgc ggccaccgcc gcgttgcctg 1081 ctgcaggtcc tgtcaacctg cccacaggca tctctcgcgt ccccactggc taccctagcg 1141 acctcaagcc agtcacctgg aaccagcacc agctggccca cctacagcag atgtgcagcg 1201 aggctagtgg gacgccagcc cctggcctga caggcaagca tgcggcagga cgcgagttgg 1261 cagggcctgg ctttgtgggc aaggcccctg cctacccgca ggaactctgc ctggcgcagt 1321 ccttccatct gaagccaccc ctggaaaagc cgaccccatc cccaccagtc aacggcatgg 1381 cagccccatt ggcctacccc aatggtcact acttccaacc cctgtggaac aacattctgc 1441 ccactcccaa tagcgacagc tcggggtctc aggaccttgc catgccgttc cacggtgggc 1501 agcccacagg tgcacccctc gactgtgcgg cagctcccgg ggcccactac cgagcaggga 1561 ccgggggcgg tccagtggca agccagaaca gcttgatgca aacagtggat tacctaagtg 1621 gggatttcca acaggcctgc ttccgagaac agagcctggc catgctgagc aaggcccacc 1681 gagcccctgg caaccgagcc cccgatccca cagagagtcg aagtcttcat attcagcacc 1741 cagggtatag atacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1801 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1861 caatcaacct ctggatta