Transcript: Human XM_017024835.2

PREDICTED: Homo sapiens family with sequence similarity 222 member B (FAM222B), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM222B (55731)
Length:
6705
CDS:
3110..4414

Additional Resources:

NCBI RefSeq record:
XM_017024835.2
NBCI Gene record:
FAM222B (55731)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017024835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000246352 ACCGTGTCTACCTCAACTATC pLKO_005 3452 CDS 100% 10.800 15.120 N Fam222b n/a
2 TRCN0000138039 GCCAGAACAGCTTGATGCAAA pLKO.1 4242 CDS 100% 4.950 3.465 N FAM222B n/a
3 TRCN0000297453 GCCAGAACAGCTTGATGCAAA pLKO_005 4242 CDS 100% 4.950 3.465 N FAM222B n/a
4 TRCN0000138920 GTGCACCAGATCAACCAGTTT pLKO.1 3530 CDS 100% 4.950 3.465 N FAM222B n/a
5 TRCN0000297452 GTGCACCAGATCAACCAGTTT pLKO_005 3530 CDS 100% 4.950 3.465 N FAM222B n/a
6 TRCN0000138699 GCAGTCTGCTCATCAATGCAA pLKO.1 3624 CDS 100% 3.000 2.100 N FAM222B n/a
7 TRCN0000138047 CATTGTCAAAGTGCCAGCCAA pLKO.1 3034 5UTR 100% 2.640 1.848 N FAM222B n/a
8 TRCN0000278429 CATTGTCAAAGTGCCAGCCAA pLKO_005 3034 5UTR 100% 2.640 1.848 N FAM222B n/a
9 TRCN0000246353 GTCGCAGTCTGCTCATCAATG pLKO_005 3621 CDS 100% 10.800 6.480 N Fam222b n/a
10 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 67 5UTR 100% 4.950 2.475 Y n/a
11 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 1653 5UTR 100% 13.200 6.600 Y IQCC n/a
12 TRCN0000180546 GCCTGTAATCCCAGGACTTTA pLKO.1 1956 5UTR 100% 13.200 6.600 Y PRR11 n/a
13 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 1751 5UTR 100% 10.800 5.400 Y SMIM11A n/a
14 TRCN0000161892 GCCTGTAATTCCAGCTACTTA pLKO.1 1721 5UTR 100% 5.625 2.813 Y GPN2 n/a
15 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2122 5UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017024835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03642 pDONR223 100% 77.2% 77.2% None 0_1ins384 n/a
2 ccsbBroad304_03642 pLX_304 0% 77.2% 77.2% V5 0_1ins384 n/a
3 TRCN0000475611 CCGCGTTTTAGGGAGTACTCTCTA pLX_317 17.2% 77.2% 77.2% V5 0_1ins384 n/a
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