Construct: ORF ccsbBroad304_03643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010855.1_s304c1, BRDN0000400369
- Derived from:
- ccsbBroadEn_03643
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS35 (55737)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03643
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55737 | VPS35 | VPS35 retromer complex comp... | NM_018206.6 | 99.9% | 100% | 1938C>T |
| 2 | human | 55737 | VPS35 | VPS35 retromer complex comp... | XM_011523227.3 | 96.3% | 96.3% | 0_1ins87;1851C>T |
| 3 | human | 55737 | VPS35 | VPS35 retromer complex comp... | XM_005256045.3 | 91.5% | 91.5% | 0_1ins201;1737C>T |
| 4 | mouse | 65114 | Vps35 | VPS35 retromer complex comp... | NM_022997.4 | 91.4% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2454
- ORF length:
- 2388
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tacaacacag cagtcccctc aggatgagca ggaaaagctc ttggatgaag 121 ccatacaggc tgtgaaggtc cagtcattcc aaatgaagag atgcctggac aaaaacaagc 181 ttatggatgc tctaaaacat gcttctaata tgcttggtga actccggact tctatgttat 241 caccaaagag ttactatgaa ctttatatgg ccatttctga tgaactgcac tacttggagg 301 tctacctgac agatgagttt gctaaaggaa ggaaagtggc agatctctac gaacttgtac 361 agtatgctgg aaacattatc ccaaggcttt accttttgat cacagttgga gttgtatatg 421 tcaagtcatt tcctcagtcc aggaaggata ttttgaaaga tttggtagaa atgtgccgtg 481 gtgtgcaaca tcccttgagg ggtctgtttc ttcgaaatta ccttcttcag tgtaccagaa 541 atatcttacc tgatgaagga gagccaacag atgaagaaac aactggtgac atcagtgatt 601 ccatggattt tgtactgctc aactttgcag aaatgaacaa gctctgggtg cgaatgcagc 661 atcagggaca tagccgagat agagaaaaaa gagaacgaga aagacaagaa ctgagaattt 721 tagtgggaac aaatttggtg cgcctcagtc agttggaagg tgtaaatgtg gaacgttaca 781 aacagattgt tttgactggc atattggagc aagttgtaaa ctgtagggat gctttggctc 841 aagaatatct catggagtgt attattcagg ttttccctga tgaatttcac ctccagactt 901 tgaatccttt tcttcgggcc tgtgctgagt tacaccagaa tgtaaatgtg aagaacataa 961 tcattgcttt aattgataga ttagctttat ttgctcaccg tgaagatgga cctggaatcc 1021 cagcggatat taaacttttt gatatatttt cacagcaggt ggctacagtg atacagtcta 1081 gacaagacat gccttcagag gatgttgtat ctttacaagt ctctctgatt aatcttgcca 1141 tgaaatgtta ccctgatcgt gtggactatg ttgataaagt tctagaaaca acagtggaga 1201 tattcaataa gctcaacctt gaacatattg ctaccagtag tgcagtttca aaggaactca 1261 ccagactttt gaaaatacca gttgacactt acaacaatat tttaacagtc ttgaaattaa 1321 aacattttca cccactcttt gagtactttg actacgagtc cagaaagagc atgagttgtt 1381 atgtgcttag taatgttctg gattataaca cagaaattgt ctctcaagac caggtggatt 1441 ccataatgaa tttggtatcc acgttgattc aagatcagcc agatcaacct gtagaagacc 1501 ctgatccaga agattttgct gatgagcaga gccttgtggg ccgcttcatt catctgctgc 1561 gctctgagga ccctgaccag cagtacttga ttttgaacac agcacgaaaa cattttggag 1621 ctggtggaaa tcagcggatt cgcttcacac tgccaccttt ggtatttgca gcttaccagc 1681 tggcttttcg atataaagag aattctaaag tggatgacaa atgggaaaag aaatgccaga 1741 agattttttc atttgcccac cagactatca gtgctttgat caaagcagag ctggcagaat 1801 tgcccttaag actttttctt caaggagcac tagctgctgg ggaaattggt tttgaaaatc 1861 atgagacagt cgcatatgaa ttcatgtccc aggcattttc tctgtatgaa gatgaaatca 1921 gcgattccaa agcacagcta gctgccatca ccttgatcat tggcactttt gaaaggatga 1981 agtgcttcag tgaagagaat catgaacctc tgaggactca gtgtgccctt gctgcatcca 2041 aacttctaaa gaaacctgat cagggccgag ctgtgagcac ctgtgcacat ctcttctggt 2101 ctggcagaaa cacggacaaa aatggggagg agcttcacgg aggcaagagg gtaatggagt 2161 gcctaaaaaa agctctaaaa atagcaaatc agtgcatgga cccctctcta caagtgcagc 2221 tttttataga aattctgaac agatatatct atttttatga aaaggaaaat gatgcggtaa 2281 caattcaggt tttaaaccag cttatccaaa agattcgaga agacctcccg aatcttgaat 2341 ccagtgaaga aacagagcag attaacaaac attttcataa cacactggag catttgcgct 2401 tgcggcggga atcaccagaa tccgaggggc caatttatga aggtctcatc ctttacccaa 2461 ttttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2521 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta