Construct: ORF ccsbBroad304_03656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011837.1_s304c1, BRDN0000401380
- Derived from:
- ccsbBroadEn_03656
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCN3B (55800)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03656
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55800 | SCN3B | sodium voltage-gated channe... | NM_001040151.1 | 100% | 100% | |
| 2 | human | 55800 | SCN3B | sodium voltage-gated channe... | NM_018400.3 | 100% | 100% | |
| 3 | human | 55800 | SCN3B | sodium voltage-gated channe... | XM_011542897.2 | 100% | 100% | |
| 4 | mouse | 235281 | Scn3b | sodium channel, voltage-gat... | NM_001083917.1 | 89.4% | 97.6% | (many diffs) |
| 5 | mouse | 235281 | Scn3b | sodium channel, voltage-gat... | NM_178227.4 | 89.4% | 97.6% | (many diffs) |
| 6 | mouse | 235281 | Scn3b | sodium channel, voltage-gat... | XM_006510234.1 | 89.4% | 97.6% | (many diffs) |
| 7 | mouse | 235281 | Scn3b | sodium channel, voltage-gat... | NM_001286614.1 | 72.7% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 714
- ORF length:
- 645
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgccttc aatagattgt ttcccctggc ttctctcgtg cttatctact 121 gggtcagtgt ctgcttccct gtgtgtgtgg aagtgccctc ggagacggag gccgtgcagg 181 gcaaccccat gaagctgcgc tgcatctcct gcatgaagag agaggaggtg gaggccacca 241 cggtggtgga atggttctac aggcccgagg gcggtaaaga tttccttatt tacgagtatc 301 ggaatggcca ccaggaggtg gagagcccct ttcaggggcg cctgcagtgg aatggcagca 361 aggacctgca ggacgtgtcc atcactgtgc tcaacgtcac tctgaacgac tctggcctct 421 acacctgcaa tgtgtcccgg gagtttgagt ttgaggcgca tcggcccttt gtgaagacga 481 cgcggctgat ccccctaaga gtcaccgagg aggctggaga ggacttcacc tctgtggtct 541 cagaaatcat gatgtacatc cttctggtct tcctcacctt gtggctgctc atcgagatga 601 tatattgcta cagaaaggtc tcaaaagccg aagaggcagc ccaagaaaac gcgtctgact 661 accttgccat cccatctgag aacaaggaga actctgcggt accagtggag gaattgccaa 721 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 781 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta