Construct: ORF ccsbBroad304_03828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009630.1_s304c1, BRDN0000462297
- Derived from:
- ccsbBroadEn_03828
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGN (57530)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03828
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57530 | CGN | cingulin | NM_020770.3 | 100% | 100% | |
| 2 | human | 57530 | CGN | cingulin | XM_005245365.5 | 100% | 100% | |
| 3 | human | 57530 | CGN | cingulin | XR_921902.2 | 65.1% | 1_77del;2180_2184delCAAGG;2488_2489ins1203 | |
| 4 | mouse | 70737 | Cgn | cingulin | NM_001037711.2 | 85% | 86.2% | (many diffs) |
| 5 | mouse | 70737 | Cgn | cingulin | XM_006502061.2 | 85% | 86.2% | (many diffs) |
| 6 | mouse | 70737 | Cgn | cingulin | XM_006502062.3 | 85% | 86.2% | (many diffs) |
| 7 | mouse | 70737 | Cgn | cingulin | NM_001293727.1 | 84.4% | 85.7% | (many diffs) |
| 8 | mouse | 545551 | BC021767 | cDNA sequence BC021767; cin... | NR_033629.1 | 16.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3678
- ORF length:
- 3609
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcaggca cccaacatgg ctgagccccg gggccccgta gaccatggag 121 tccagattcg cttcatcaca gagccagtga gtggtgcaga gatgggcact ctacgtcgag 181 gtggacgacg cccagctaag gatgcaagag ccagtaccta cggggttgct gtgcgtgtgc 241 agggaatcgc tgggcagccc tttgtggtgc tcaacagtgg ggagaaaggc ggtgactcct 301 ttggggtcca aatcaagggg gccaatgacc aaggggcctc aggagctctg agctcagatt 361 tggaactccc tgagaacccc tactctcagg tcaagggatt tcctgccccc tcgcagagca 421 gcacatctga tgaggagcct ggggcctact ggaatggaaa gctactccgt tcccactccc 481 aggcctcact ggcaggccct ggcccagtgg atcctagtaa cagaagcaac agcatgctgg 541 agctagcccc gaaagtggct tccccaggta gcaccattga cactgctccc ctgtcttcag 601 tggactcact catcaacaag tttgacagtc aacttggagg ccaggcccgg ggtcggactg 661 gccgccgaac acggatgcta ccccctgaac agcgcaaacg gagcaagagc ctggacagcc 721 gcctcccacg ggacaccttt gaggaacggg agcgccagtc caccaaccac tggacctcta 781 gcacaaaata tgacaaccat gtgggcactt cgaagcagcc agcccagagc cagaacctga 841 gtcctctcag tggctttagc cgttctcgtc agactcagga ctgggtcctt cagagttttg 901 aggagccgcg gaggagtgca caggacccca ccatgctgca gttcaaatca actccagacc 961 tccttcgaga ccagcaggag gcagccccac caggcagtgt ggaccatatg aaggccacca 1021 tctatggcat cctgagggag ggaagctcag aaagtgaaac ctctgtgagg aggaaggtta 1081 gtttggtgct ggagaagatg cagcctctag tgatggtttc ttctggttct actaaggccg 1141 tggcagggca gggtgagctt acccgaaaag tggaggagct acagcgaaag ctggatgaag 1201 aggtgaagaa gcggcagaag ctagagccat cccaagttgg gctggagcgg cagctggagg 1261 agaaaacaga agagtgcagc cgactgcagg agctgctgga gaggaggaag ggggaggccc 1321 agcagagcaa caaggagctc cagaacatga agcgcctctt ggaccagggt gaagatttac 1381 gacatgggct ggagacccag gtgatggagc tgcagaacaa gctgaaacat gtccagggtc 1441 ctgagcctgc taaggaggtg ttactgaagg acctgttaga gacccgggaa cttctggaag 1501 aggtcttgga ggggaaacag cgagtagagg agcagctgag gctgcgggag cgggagttga 1561 cagccctgaa gggggccctg aaagaggagg tagcctcccg tgaccaggag gtggaacatg 1621 tccggcagca gtaccagcga gacacagagc agctccgcag gagcatgcaa gatgcaaccc 1681 aggaccatgc agtgctggag gccgagaggc agaagatgtc agcccttgtg cgagggctgc 1741 agagggagct ggaggagact tcagaggaga cagggcattg gcagagtatg ttccagaaga 1801 acaaggagga tcttagagcc accaagcagg aactcctgca gctgcgaatg gagaaggagg 1861 agatggaaga ggagcttgga gagaagatag aggtcttgca gagggaatta gagcaggccc 1921 gagctagtgc tggagatact cgccaggttg aggtgctcaa gaaggagctg ctccggacac 1981 aggaggagct taaggaactg caggcagaac ggcagagcca ggaggtggct gggcgacacc 2041 gggaccggga gttggagaag cagctggcgg tcctgagggt cgaggctgat cgaggtcggg 2101 agctggaaga acagaacctc cagctacaaa agaccctcca gcaactgcga caggactgtg 2161 aagaggcttc caaggctaag atggtggccg aggcagaggc aacagtgctg gggcagcggc 2221 gggccgcagt ggagacgacg cttcgggaga cccaggagga aaatgacgaa ttccgccggc 2281 gcatcctggg tttggagcag cagctgaagg agactcgagg tctggtggat ggtggggaag 2341 cggtggaggc acgactacgg gacaagctgc agcggctgga ggcagagaaa cagcagctgg 2401 aggaggccct gaatgcgtcc caggaagagg aggggagtct ggcagcagcc aagcgggcac 2461 tggaggcacg cctagaggag gctcagcggg ggctggcccg cctggggcag gagcagcaga 2521 cactgaaccg ggccctggag gaggaaggga agcagcggga ggtgctccgg cgaggcaagg 2581 ctgagctgga ggagcagaag cgtttgctgg acaggactgt ggaccgactg aacaaggagt 2641 tggagaagat cggggaggac tctaagcaag ccctgcagca gctccaggcc cagctggagg 2701 attataagga aaaggcccgg cgggaggtgg cagatgccca gcgccaggcc aaggattggg 2761 ccagtgaggc tgagaagacc tctggaggac tgagccgact tcaggatgag atccagaggc 2821 tgcggcaggc cctgcaggca tcccaggctg agcgggacac agcccggctg gacaaagagc 2881 tactggccca gcgactgcag gggctggagc aagaggcaga gaacaagaag cgttcccagg 2941 acgacagggc ccggcagctg aagggtctcg aggaaaaagt ctcacggctg gaaacagagt 3001 tagatgagga gaagaacacc gtggagctgc taacagatcg ggtgaatcgt ggccgggacc 3061 aggtggatca gctgaggaca gagctcatgc aggaaaggtc tgctcggcag gacctggagt 3121 gtgacaaaat ctccttggag agacagaaca aggacctgaa gacccggttg gccagctcag 3181 aaggcttcca gaagcctagt gccagcctct ctcagcttga gtcccagaat cagttgttgc 3241 aggagcggct acaggctgaa gagagggaga agacagttct gcagtctacc aatcgaaaac 3301 tggagcggaa agttaaagaa ctatccatcc agattgaaga cgagcggcag catgtcaatg 3361 accagaaaga ccagctaagc ctgagggtga aggctttgaa gcgtcaggtg gatgaagcag 3421 aagaggaaat tgagcgactg gacggcctga ggaagaaggc ccagcgtgag gtggaggagc 3481 agcatgaggt caatgaacag ctccaggccc ggatcaagtc tctggagaag gactcctggc 3541 gcaaagcttc ccgctcagct gctgagtcag ctctcaaaaa cgaagggctg agctcagatg 3601 aggaattcga cagtgtctac gatccctcgt ccattgcatc actgcttacg gagagcaacc 3661 tacagaccag ctcctgtttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 3721 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3781 agtcgacaat caacctctgg atta