Construct: ORF ccsbBroad304_04058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004562.1_s304c1, BRDN0000399802
- Derived from:
- ccsbBroadEn_04058
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHF1 (79156)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04058
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79156 | PLEKHF1 | pleckstrin homology and FYV... | NM_024310.5 | 100% | 100% | |
| 2 | human | 79156 | PLEKHF1 | pleckstrin homology and FYV... | XM_011527309.3 | 100% | 100% | |
| 3 | human | 79156 | PLEKHF1 | pleckstrin homology and FYV... | XM_005259256.3 | 76.6% | 76.6% | 1_255del |
| 4 | mouse | 72287 | Plekhf1 | pleckstrin homology domain ... | NM_024413.2 | 84.2% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggaccacttg gccaacacgg agatcaacag ccagcgcatc gcggcagtgg 121 agagctgctt cggggcctcg gggcagccgc tggcgctgcc aggccgagtg ctgctgggcg 181 agggcgtgct gaccaaagag tgccgcaaga aggccaagcc gcgcatcttc ttcctcttta 241 acgacatcct ggtgtatggc agcatcgtgc tcaacaagcg caagtaccgc agccagcaca 301 tcatccccct ggaggaggtc acactggagc tgttgccgga gacgctgcag gccaagaacc 361 gctggatgat caagacggcc aagaagtcct ttgtggtgtc ggccgcctcc gctacggagc 421 gccaggaatg gattagccac atcgaggagt gcgtgcggcg gcaactgagg gccacgggcc 481 gcccgcccag cacggagcac gcggcaccct ggatccccga caaggccacg gacatctgca 541 tgcgctgcac gcagacgcgc ttctctgccc tcacgaggcg ccaccactgc cgcaagtgcg 601 gcttcgtggt ctgcgctgag tgctcgcgcc agcgcttcct gctcccgcgc ctgtccccca 661 agcccgtgcg cgtctgcagc ctctgctacc gcgaactggc cgcccagcag cggcaggagg 721 aggcggagga gcagggcgcg gggtccccag ggcagccagc ccacctggcc cggcccatct 781 gcggagcgtc cagtggagat gacgatgact ccgacgagga caaggagggc agcagggacg 841 gcgactggcc cagcagcgtg gagttctacg cctcgggggt ggcctggtct gccttccaca 901 gctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 961 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1021 tctggatta