Transcript: Mouse NM_024413.2

Mus musculus pleckstrin homology domain containing, family F (with FYVE domain) member 1 (Plekhf1), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Plekhf1 (72287)
Length:
1559
CDS:
71..910

Additional Resources:

NCBI RefSeq record:
NM_024413.2
NBCI Gene record:
Plekhf1 (72287)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_024413.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000340988 TCAACGACATCCTGGTGTATG pLKO_005 243 CDS 100% 10.800 7.560 N Plekhf1 n/a
2 TRCN0000340989 CTTGGGTTTCAGAGTCCATTC pLKO_005 1180 3UTR 100% 6.000 4.200 N Plekhf1 n/a
3 TRCN0000121406 CAAGAACCGATGGATGATCAA pLKO.1 358 CDS 100% 4.950 3.465 N Plekhf1 n/a
4 TRCN0000340986 CAAGAACCGATGGATGATCAA pLKO_005 358 CDS 100% 4.950 3.465 N Plekhf1 n/a
5 TRCN0000340913 CGGACAAGGCCACGGACATTT pLKO_005 522 CDS 100% 4.400 3.080 N Plekhf1 n/a
6 TRCN0000121405 CAGGTGGAGTTCTATGCTTCT pLKO.1 860 CDS 100% 4.050 2.835 N Plekhf1 n/a
7 TRCN0000340987 CAGGTGGAGTTCTATGCTTCT pLKO_005 860 CDS 100% 4.050 2.835 N Plekhf1 n/a
8 TRCN0000166611 CCAAGAAGTCCTTTGTGGTGT pLKO.1 384 CDS 100% 2.640 1.848 N PLEKHF1 n/a
9 TRCN0000166466 CAAGAAGTCCTTTGTGGTGTC pLKO.1 385 CDS 100% 2.250 1.575 N PLEKHF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024413.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04058 pDONR223 100% 84.2% 89.9% None (many diffs) n/a
2 ccsbBroad304_04058 pLX_304 0% 84.2% 89.9% V5 (many diffs) n/a
3 TRCN0000468447 GACCTGTTTCGTCGAAATTTACAG pLX_317 26.8% 84.2% 89.9% V5 (many diffs) n/a
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