Transcript: Human NM_024310.5

Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
PLEKHF1 (79156)
Length:
1699
CDS:
62..901

Additional Resources:

NCBI RefSeq record:
NM_024310.5
NBCI Gene record:
PLEKHF1 (79156)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024310.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000431032 GGTAACAAACGCCACCTTACA pLKO_005 1135 3UTR 100% 4.950 6.930 N PLEKHF1 n/a
2 TRCN0000163979 CTTTAACGACATCCTGGTGTA pLKO.1 232 CDS 100% 4.050 5.670 N PLEKHF1 n/a
3 TRCN0000425012 CCACAGGTCTTGGTAACAAAC pLKO_005 1124 3UTR 100% 10.800 7.560 N PLEKHF1 n/a
4 TRCN0000165370 GATGATCAAGACGGCCAAGAA pLKO.1 361 CDS 100% 4.950 3.465 N PLEKHF1 n/a
5 TRCN0000429627 AGCAGGTTTGGGAACACAGAG pLKO_005 1233 3UTR 100% 4.050 2.835 N PLEKHF1 n/a
6 TRCN0000417119 AGGTAATGCCTTTCCCTTCAG pLKO_005 1088 3UTR 100% 4.050 2.835 N PLEKHF1 n/a
7 TRCN0000414485 GTATGGCAGCATCGTGCTCAA pLKO_005 250 CDS 100% 4.050 2.835 N PLEKHF1 n/a
8 TRCN0000427068 GTCCAGTGGAGATGACGATGA pLKO_005 784 CDS 100% 4.050 2.835 N PLEKHF1 n/a
9 TRCN0000166611 CCAAGAAGTCCTTTGTGGTGT pLKO.1 375 CDS 100% 2.640 1.848 N PLEKHF1 n/a
10 TRCN0000165241 GAATGGATTAGCCACATCGAG pLKO.1 422 CDS 100% 2.640 1.848 N PLEKHF1 n/a
11 TRCN0000166466 CAAGAAGTCCTTTGTGGTGTC pLKO.1 376 CDS 100% 2.250 1.575 N PLEKHF1 n/a
12 TRCN0000163236 GCGCATCTTCTTCCTCTTTAA pLKO.1 217 CDS 100% 13.200 7.920 N PLEKHF1 n/a
13 TRCN0000435009 GATGGGAGTGTAGCCACAGAA pLKO_005 1263 3UTR 100% 4.950 2.970 N PLEKHF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024310.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04058 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04058 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468447 GACCTGTTTCGTCGAAATTTACAG pLX_317 26.8% 100% 100% V5 n/a
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