Construct: ORF ccsbBroad304_04116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002403.1_s304c1, BRDN0000411015
- Derived from:
- ccsbBroadEn_04116
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIN28A (79727)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04116
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79727 | LIN28A | lin-28 homolog A | NM_024674.6 | 100% | 100% | |
| 2 | human | 79727 | LIN28A | lin-28 homolog A | XM_011542148.2 | 100% | 100% | |
| 3 | mouse | 83557 | Lin28a | lin-28 homolog A (C. elegans) | NM_145833.1 | 91% | 96.6% | (many diffs) |
| 4 | mouse | 83557 | Lin28a | lin-28 homolog A (C. elegans) | XM_011250361.2 | 69.3% | 73.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctccgtgtcc aaccagcagt ttgcaggtgg ctgcgccaag gcggcagaag 121 aggcgcccga ggaggcgccg gaggacgcgg cccgggcggc ggacgagcct cagctgctgc 181 acggtgcggg catctgtaag tggttcaacg tgcgcatggg gttcggcttc ctgtccatga 241 ccgcccgcgc cggggtcgcg ctcgaccccc cagtggatgt ctttgtgcac cagagtaagc 301 tgcacatgga agggttccgg agcttgaagg agggtgaggc agtggagttc acctttaaga 361 agtcagccaa gggtctggaa tccatccgtg tcaccggacc tggtggagta ttctgtattg 421 ggagtgagag gcggccaaaa ggaaagagca tgcagaagcg cagatcaaaa ggagacaggt 481 gctacaactg tggaggtcta gatcatcatg ccaaggaatg caagctgcca ccccagccca 541 agaagtgcca cttctgccag agcatcagcc atatggtagc ctcatgtccg ctgaaggccc 601 agcagggccc tagtgcacag ggaaagccaa cctactttcg agaggaagaa gaagaaatcc 661 acagccctac cctgctcccg gaggcacaga attgcccaac tttcttgtac aaagtggttg 721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 781 accggtggcg cgttaagtcg acaatcaacc tctggatta